Stim1 (NM_009287) Mouse Tagged ORF Clone

CAT#: MR210034

Stim1 (Myc-DDK-tagged) - Mouse stromal interaction molecule 1 (Stim1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stim1
• Synonyms: SIM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210034 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGTGCGCCCGTCTTGCCCTGTGGCTTCTTTGGGGGCTCCTTCTGCATCAGGGCCAGAGTCTCA
  GCCATAGTCACAGTGAAAAGAATACAGGAGCTAGCTCCGGGGCGACTTCTGAAGAGTCTACCGAAGCAGA
  GTTTTGCCGAATTGACAAGCCCCTGTGCCACAGTGAGGATGAGAAGCTCAGCTTTGAGGCCGTCCGAAAC
  ATCCATAAGCTGATGGATGACGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTAAGGG
  AAGACCTCAATTACCATGACCCAACAGTGAAACATAGCACCTTCCATGGTGAGGATAAGCTTATCAGCGT
  GGAGGACCTGTGGAAGGCGTGGAAATCATCAGAAGTGTACAACTGGACTGTGGATGAGGTGATACAGTGG
  CTCATTACGTATGTGGAGCTGCCACAGTATGAGGAGACCTTCCGGAAGTTGCAGCTTACTGGCCACGCCA
  TGCCAAGGCTAGCAGTAACCAACACCACCATGACAGGGACTGTACTGAAGATGACAGATCGGAGCCACAG
  GCAGAAGCTGCAGCTGAAGGCCCTGGACACAGTGCTGTTTGGGCCTCCTCTCTTGACTCGGCATAATCAC
  CTGAAGGACTTCATGCTGGTGGTGTCTATCGTTATTGGTGTGGGTGGCTGCTGGTTTGCCTATATCCAGA
  ACCGTTACTCTAAGGAGCACATGAAGAAAATGATGAAGGATCTGGAAGGGTTACACCGGGCTGAGCAGAG
  TCTGCATGACCTTCAGGAAAGGCTGCACAAGGCCCAGGAGGAGCACCGAACTGTGGAAGTAGAGAAAGTC
  CACCTGGAGAAGAAGCTGCGAGATGAGATCAACCTTGCCAAGCAGGAAGCTCAGCGGCTGAAGGAGCTGA
  GGGAGGGTACTGAGAATGAGAGGAGCCGTCAAAAATATGCTGAGGAAGAGCTGGAGCAGGTTCGGGAGGC
  CTTGAGGAAAGCAGAGAAGGAGCTGGAATCACACAGCTCATGGTATGCTCCTGAGGCCCTGCAGAAGTGG
  CTGCAGCTGACCCATGAGGTGGAGGTGCAGTACTACAACATCAAGAAGCAAAATGCAGAGAGGCAGCTGC
  TGGTGGCCAAGGAGGGGGCTGAGAAAATAAAAAAGAAGAGAAACACGCTTTTTGGTACCTTCCATGTGGC
  CCACAGCTCTTCCCTGGATGATGTGGATCATAAAATCCTAACTGCTAAGCAAGCTCTGAGTGAGGTGACA
  GCGGCACTGAGGGAGCGCCTGCACCGGTGGCAGCAGATCGAGATCCTCTGCGGTTTCCAGATTGTCAATA
  ACCCCGGCATCCACTCCTTGGTGGCTGCTCTCAACATCGACCCCAGCTGGATGGGCAGCACCCGCCCTAA
  CCCCGCCCACTTCATCATGACTGACGATGTGGATGACATGGATGAGGAGATTGTGTCGCCCTTGTCCATG
  CAGTCCCCCAGCCTGCAGAGCAGTGTCCGGCAGCGCCTGACGGAGCCACAGCTTGGCCTGGGATCTCAGA
  GGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCACATGAGTGACCGCCAGCGTGTGGCCCCCAAGCC
  TCCTCAGATGGGCCGTGCTGCAGATGAAGCTCTCAATGCCATGCCTTCCAATGGCAGCCATCGGCTGATT
  GAGGGGGTCCATCCAGGATCTCTGGTGGAGAAACTGCCTGACAGCCCTGCTCTGGCCAAGAAGACATTTA
  TGGCGTTGAACCATGGCCTAGACAAGGCCCACAGCCTGATGGAGCTGAACCCCTCAGTCCCACCTGGTGG
  CTCCCCACTTTTGGATTCTTCCCATTCTCTTAGCCCCAGTTCCCCAGACCCAGACACACCATCTCCAGTT
  GGGGACAACCGAGCTCTGCAGGGTAGCCGAAACACACGAATTCCCCACTTGGCTGGCAAGAAGGCAATGG
  CTGAGGAGGATAATGGTTCCATTGGTGAGGAGACAGACTCCAGTCCAGGCAGGAAGAAGTTTCCTCTCAA
  AATTTTTAAGAAGCCTCTTAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210034 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGATSEESTEAEFCRIDKPLCHSEDEKLSFEAVRN
  IHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQW
  LITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNH
  LKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKV
  HLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKW
  LQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVT
  AALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSM
  QSPSLQSSVRQRLTEPQLGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMGRAADEALNAMPSNGSHRLI
  EGVHPGSLVEKLPDSPALAKKTFMALNHGLDKAHSLMELNPSVPPGGSPLLDSSHSLSPSSPDPDTPSPV
  GDNRALQGSRNTRIPHLAGKKAMAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009287
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009287.1, NM_009287.2, NM_009287.3, NM_009287.4, NP_033313.2
• RefSeq Size: 3609 bp
• RefSeq ORF: 2058 bp
• Locus ID: 20866
• MW: 77.6 kDa