Ddr1 (BC065998) Mouse Tagged ORF Clone

CAT#: MR210041

Ddr1 (Myc-DDK-tagged) - Mouse discoidin domain receptor family, member 1 (cDNA clone MGC:90037 IMAGE:5686085)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddr1
• Synonyms: 6030432F18, CD167a, PTK3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210041 representing BC065998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGGGACCCTCTCATCTCTACTGCTGCTGCTACTCTTGGTGACAATTGGAGATGCTGACATGA
  AGGGACATTTTGACCCTGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGCACCATTCCTGACAGCGA
  TATCTCTGTGTCCAGCTCCTGGTCGGACTCTACCGCTGCCCGCCACAGCAGGCTGGAAAGCAGTGATGGA
  GATGGGGCTTGGTGCCCTGCAGGGCCTGTGTTCCCCAAAGAAGAGGAGTACTTGCAGGTGGACCTTCGTA
  GGCTACACCTGGTGGCTCTGGTGGGCACCCAGGGCCGCCATGCTGGGGGTCTGGGCAAAGAGTTCTCCCG
  AAGCTATCGGTTGCGTTACTCCCGAGATGGCCGCCGCTGGATGGACTGGAAGGACCGCTGGGGACAGGAG
  GTGATTTCGGGTAACGAGGATCCCGGGGGAGTAGTGCTGAAGGACCTTGGGCCCCCCATGGTGGCCCGGC
  TGGTCCGCTTCTACCCCAGGGCTGACCGGGTCATGAGTGTCTGTCTTCGGGTGGAGCTCTATGGCTGCCT
  CTGGCGGGATGGACTCCTGTCATATACAGCCCCCGTGGGGCAGACCATGCAGTTATCTGAGGTGATGGTA
  CATCTCAATGATTCCACTTACGATGGATATACTGCTGGAGGGCTGCAGTATGGCGGTCTGGGCCAGCTGG
  CAGATGGCGTGGTGGGCCTGGATGATTTCAGGCAGAGCCAGGAGCTGCGGGGCTGGCCAGGCTATGACTA
  TGTGGGATGGAGCAATCAGAGCTTCCCCACGGGCTACGTGGAGATGGAGTTTGAGTTTGATCGGTTGAGG
  ACCTTCCAGACCATGCAGGTCCACTGTAACAACATGCACACTCTGGGAGCCCGCCTACCAGGCGGGGTGG
  AATGCCGGTTTAAAAGGGGTCCCGCCATGGCCTGGGAAGGAGAGCCTGTCCGCCATGCTCTGGGAGGCAG
  CCTTGGAGACCCCAGAGCCCGGGCCATCTCAGTGCCCCTGGGTGGCCACGTGGGCCGCTTTCTGCAGTGC
  AGATTCCTCTTTGCAGGTCCTTGGTTACTCTTCAGTGAGATCTCTTTCATCTCAGATGTGGTGAACGACT
  CCTCTGACACCTTCCCACCAGCCCCCTGGTGGCCACCTGGCCCGCCTCCCACCAACTTCAGCAGCTTGGA
  GCTGGAGCCCCGGGGTCAACAGCCAGTGGCCAAGGCGGAGGGGAGCCCAACTGCCATCCTCATTGGCTGC
  CTGGTGGCCATCATCCTGCTGCTGCTTCTCATCATCGCGCTGATGCTCTGGAGGCTGCACTGGCGCCGGC
  TGCTCAGCAAGGCTGAGCGCCGCGTGTTGGAGGAGGAGCTGACGGTTCACCTTTCTGTCCCTGGGGACAC
  CATCCTCATCAACAACCGCCCAGGACCCCGAGAGCCACCCCCTTACCAGGAGCCCCGGCCTCGGGGGACT
  CCACCCCATTCTGCACCCTGCGTCCCCAACGGCTCTGCCTGCAGTGGGGACTATATGGAGCCCGAGAAGC
  CGGGTGCCCCGCTTCTACCCCCACCTCCCCAGAACAGCGTCCCCCATTATGCCGAAGCTGACATTGTCAC
  CCTGCAGGGCGTCACTGGGGGCAACACCTACGCTGTGCCTGCACTGCCCCCAGGGGCGGTTGGGGATGGG
  CCCCCCAGAGTGGATTTCCCTCGGTCACGGCTCCGCTTCAAGGAGAAGCTTGGCGAGGGCCAATTTGGGG
  AGGTACACCTGTGTGAAGTAGAGGACCCGGCAGCCCTTTCCCAATGGGAGCCATCCAGGGGAAGCTGGAC
  TCGAAAACCAGAGAAGCCTATGGCACCGCACCCCACCGTCTCCATACTTGCCCATTCCCAAGAGGCAGTG
  TCTCTGCGGGTGGGCTGGGCCTGCCCGAGGAGCAGATACACACACTCCCTTCCGTTTCTTCCTTCCATCT
  GCCAAGTGCCACCCTCCCAGCTGGCCCTGTGGTTGGGATCCTGCCTGACTTCTTCCAGCTTTCTTCTTGT
  CACACACTGGACGCCGCCAGCTGAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210041 representing BC065998
  Red=Cloning site Green=Tags(s)
  
  MGTGTLSSLLLLLLLVTIGDADMKGHFDPAKCRYALGMQDRTIPDSDISVSSSWSDSTAARHSRLESSDG
  DGAWCPAGPVFPKEEEYLQVDLRRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMDWKDRWGQE
  VISGNEDPGGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMQLSEVMV
  HLNDSTYDGYTAGGLQYGGLGQLADGVVGLDDFRQSQELRGWPGYDYVGWSNQSFPTGYVEMEFEFDRLR
  TFQTMQVHCNNMHTLGARLPGGVECRFKRGPAMAWEGEPVRHALGGSLGDPRARAISVPLGGHVGRFLQC
  RFLFAGPWLLFSEISFISDVVNDSSDTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTAILIGC
  LVAIILLLLLIIALMLWRLHWRRLLSKAERRVLEEELTVHLSVPGDTILINNRPGPREPPPYQEPRPRGT
  PPHSAPCVPNGSACSGDYMEPEKPGAPLLPPPPQNSVPHYAEADIVTLQGVTGGNTYAVPALPPGAVGDG
  PPRVDFPRSRLRFKEKLGEGQFGEVHLCEVEDPAALSQWEPSRGSWTRKPEKPMAPHPTVSILAHSQEAV
  SLRVGWACPRSRYTHSLPFLPSICQVPPSQLALWLGSCLTSSSFLLVTHWTPPAEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC065998
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC065998, AAH65998
• RefSeq Size: 2582 bp
• RefSeq ORF: 2060 bp
• Locus ID: 12305
• MW: 94.6 kDa