Mtrr (NM_172480) Mouse Tagged ORF Clone

CAT#: MR210099

Mtrr (Myc-DDK-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtrr
• Synonyms: 4732420G08; MSR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210099 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAGGTTTTTGTTACTTTATGCTACACAGCGAGGACAGGCAAAGGCCATAGCAGAAGAAATAAGTG
  AGCAGGCTGTGTCACACGGGTTTTCCGCAGATCTTCACTGTATCAGTGAGTCGGAAAAGTATGATCTGAA
  AACAGAAACTGGCCCTCTGGTGATGGTTGTATCTACCACGGGTACAGGAGACCCACCTGACACAGCCCGG
  AAGTTTGTTAAAGAAATACACAACAAGACACTCCCCACGGACTATTTTGCTCACCTGCGGTATGGATTAT
  TGGGCTTGGGTGACTCAGAATACACATACTTCTGCAATGGAGGGAAAGTAATTGATAAACGACTTCAGGA
  GCTTGGAGCCCAGCGTTTCTACGACACCGGACATGCCGATGACTGTGTCGGTTTAGAGCTTGTGGTAGAG
  CCGTGGATTGATGGACTCTGGGCTGCTCTCACAAAGCACTTTAAGTCACTTGGAGGACAAGAAAACATGA
  GTGACACACTCTCGCGAGCATCAGATGCCCCCTTGAGCACTGCCATGAAGCCGGAGTTGTTGCACATCCA
  GTCACAATTTGAACTGCTGAGATTAGAGGACGTGGGGGAGAGGGATTCTGAGCTTCGGGAACAAAATGAG
  ACAAACAGGGGTCAGCAGGGTCGGATCGAAGACTTTGACTCCTCGCTTGTGCACTCGGTCCCCCCACTCT
  CCCAGAGCTCTCTAAGTATTCCTGCTGTGCCCCCAGAGTATTTGGAGGTCCATCTTCAAGAGTCTCTTGG
  CCAGGAGGAAAACCAAGCATCTGTGCCTTCAGGGGATCCAAGTTTTCAAGTTCCAATTTCAAAAGCCATT
  CGTCTGACTACAAATGATGCCGTAAAGAGCACTCTGCTGTTGGAACTGGACATTTCAAAAATAGAGTTTT
  CCCATCAACCTGGAGACTCCTTCAATGTGACCTGTCCAAACAGTGATCGTGAGGTAGAAGAACTGCTCCA
  AAGGCTGCAGCTGGCTGATAAACGAGCGCACCGTGTCATCTTGAAAATTAAGACGGACACTAAGAAGAAA
  GGAGCTGCCCTGCCTGCGCATGTGCCTGAGGGGCGTTCTCTCCAGTTCATCCTCACCTGGTGTCTTGAAA
  TACGAGCAGTTCCTAAAAAGGCCTTTTTGCGGGCCCTTGCGGAGCACACCAGCAGTGCCACTGAAAAGCG
  GAGGCTGCAGGAGCTGTGCAGTAAACAAGGAGCCGCTGATTACAACCGCTTTATCCGAGATGCCAGCGTC
  TGCCTGCTGGACCTCCTGCTCACCTTCCCGTCCTGCCAGCCTCCGCTCAGCCTCCTGCTGGAGCACCTTC
  CTAAACTTCAGCCTCGACCGTACTCATGTGCAAGCTCCAGCTTACGCCACCCAGACAAGCTTCACTTCGT
  GTTTAACATCGTGGAGTTCCCACCGAGCACCACTGCAGCCTCCCCGCGGAAGGGAGTGTGCACGGGCTGG
  CTAGCCACATTGGTTGCTCCATTCCTTCAGCCAAACACAGACGTTTCGAATGCAGACAGTGGGGACACTC
  TGGCTCCTGAGATACGTATCTCTCCTCGGGCAACAAATGCTTTCCACTTACCAGAGGACCCGTCGGCCCC
  CATCATAATGGTGGGTCCAGGAACTGGCGTAGCCCCCTTTGTTGGCTTCCTCCAGCACAGAGAAAAACTT
  CAAGAACAACACCCAGATGGGAAATTTGGAGCAATGTGGCTGTTCTTTGGCTGCAGACACAAGGACAGAG
  ACTACTTGTTCAGGGAGGAGCTCAGGCATTTCCTCAAGACTGGGGTCTTGACTCATCTGAAGGTCTCGTT
  TTCAAGAGACGCTGCCCCTGACGGGGAGGAGGCCCCAGCAAAGTATGTGCAAGACAACCTCCAGCGGCAC
  AGCCAGCAGGTGGCCAGGACCCTCCTTCAGGAGAATGGCTACATTTACGTGTGTGGAGACGCCAAGAATA
  TGGCCAAGGACGTGAACGACACCCTCATAGGAATCATAAGCAATGAAGCTGGTGTTGACAAACTAGAAGC
  AATGAAGACGCTGGCAACGTTAAAGCAAGAGAAACGATATCTGCAGGACATCTGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210099 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRFLLLYATQRGQAKAIAEEISEQAVSHGFSADLHCISESEKYDLKTETGPLVMVVSTTGTGDPPDTAR
  KFVKEIHNKTLPTDYFAHLRYGLLGLGDSEYTYFCNGGKVIDKRLQELGAQRFYDTGHADDCVGLELVVE
  PWIDGLWAALTKHFKSLGGQENMSDTLSRASDAPLSTAMKPELLHIQSQFELLRLEDVGERDSELREQNE
  TNRGQQGRIEDFDSSLVHSVPPLSQSSLSIPAVPPEYLEVHLQESLGQEENQASVPSGDPSFQVPISKAI
  RLTTNDAVKSTLLLELDISKIEFSHQPGDSFNVTCPNSDREVEELLQRLQLADKRAHRVILKIKTDTKKK
  GAALPAHVPEGRSLQFILTWCLEIRAVPKKAFLRALAEHTSSATEKRRLQELCSKQGAADYNRFIRDASV
  CLLDLLLTFPSCQPPLSLLLEHLPKLQPRPYSCASSSLRHPDKLHFVFNIVEFPPSTTAASPRKGVCTGW
  LATLVAPFLQPNTDVSNADSGDTLAPEIRISPRATNAFHLPEDPSAPIIMVGPGTGVAPFVGFLQHREKL
  QEQHPDGKFGAMWLFFGCRHKDRDYLFREELRHFLKTGVLTHLKVSFSRDAAPDGEEAPAKYVQDNLQRH
  SQQVARTLLQENGYIYVCGDAKNMAKDVNDTLIGIISNEAGVDKLEAMKTLATLKQEKRYLQDIWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172480
• ORF Size: 2091 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3692 bp
• RefSeq ORF: 2091 bp
• Locus ID: 210009
• MW: 77.6 kDa
• Gene Summary: Methionine is an essential amino acid required for protein synthesis and one-carbon metabolism. Its synthesis is catalyzed by the enzyme methionine synthase. Methionine synthase eventually becomes inactive due to the oxidation of its cob(I)alamin cofactor. The protein encoded by this gene regenerates a functional methionine synthase via reductive methylation. It is a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. Mutations in a similar gene in human have been associated with cblE complementation type homocystinuria-megaloblastic anemia and susceptibility to folate-sensitive neural tube defects. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2015]