Hhip (NM_020259) Mouse Tagged ORF Clone
CAT#: MR210126
- TrueORF®
Hhip (Myc-DDK-tagged) - Mouse Hedgehog-interacting protein (Hhip)
"NM_020259" in other vectors (4)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hhip |
Synonyms | Hhip1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210126 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGATGCTCTCGTTTAAGCTGCTACTGCTGGCCGTGGCTCTGGGCTTCTTTGAAGGAGATGCGA AGTTTGGGGAAAGGAACGAGGGGAGCGGAGCGAGAAGGAGACGGTGCCTGAATGGGAACCCCCCAAAGCG CCTAAAGAGAAGGGACAGGCGGGTGATGTCCCAGCTGGAGCTGCTCAGTGGAGGAGAGATCCTGTGTGGT GGCTTCTACCCACGAGTATCTTGCTGCCTGCAGAGTGACAGCCCTGGATTGGGGCGTCTGGAGAACAAGA TCTTTTCTGCCACCAACAACTCAGAATGCAGCAGGCTGCTGGAGGAGATCCAATGTGCTCCCTGCTCCCC GCATTCCCAGAGCCTCTTCTACACACCTGAAAGAGATGTCCTGGATGGGGACCTAGCACTTCCACTCCTC TGCAAAGACTACTGCAAAGAATTCTTTTATACTTGCCGAGGCCATATTCCAGGTCTTCTTCAAACAACTG CTGATGAATTTTGCTTTTACTATGCAAGAAAAGATGCTGGGTTATGCTTTCCAGACTTCCCGAGAAAGCA AGTCAGAGGACCAGCATCTAACTACTTGGGCCAGATGGAAGACTACGAGAAAGTGGGGGGGATCAGCAGA AAACACAAACACAACTGCCTCTGTGTCCAGGAGGTCATGAGTGGGCTGCGGCAGCCTGTGAGCGCTGTGC ACAGCGGGGATGGCTCCCATCGGCTCTTCATTCTAGAGAAGGAAGGCTACGTGAAGATTCTAACCCCAGA AGGAGAACTGTTCAAGGAGCCTTACTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAC GAAAGGGGCCTGCTAAGCCTGGCATTCCATCCCAATTACAAGAAAAATGGAAAGCTGTATGTGTCTTATA CCACCAACCAGGAACGGTGGGCTATTGGGCCTCACGACCACATTCTTCGGGTTGTGGAATACACAGTATC CAGGAAAAACCCCCATCAAGTTGATGTGAGAACAGCCAGGGTGTTTCTGGAAGTCGCAGAGCTCCACCGA AAGCATCTTGGGGGACAGCTGCTCTTTGGTCCTGATGGCTTTTTGTACATCATCCTTGGGGATGGTATGA TCACATTGGATGACATGGAAGAGATGGATGGGTTAAGTGACTTCACAGGCTCTGTGCTGAGGCTGGACGT GGACACCGACATGTGCAATGTGCCTTATTCCATACCTCGGAGTAACCCTCACTTCAACAGCACCAACCAG CCCCCAGAAGTATTTGCCCACGGCCTCCATGATCCAGGCAGATGTGCCGTGGATCGACATCCTACTGATA TAAACATCAATTTAACAATACTTTGCTCAGATTCCAACGGGAAAAACAGGTCATCAGCCAGAATCCTACA GATAATAAAGGGAAGAGGTTATGAAAGTGAGCCATCTCTTCTTGAATTCAAGCCATTCAGTAACGGCCCT TTGGTTGGTGGATTTGTTTACAGAGGCTGTCAGTCTGAAAGATTGTACGGAAGCTATGTGTTCGGAGATC GCAATGGGAATTTCTTAACCCTCCAGCAAAGCCCAGTGACCAAGCAATGGCAAGAAAAGCCGCTCTGCCT GGGTGCCAGCAGCTCCTGTCGAGGCTACTTTTCGGGTCACATCTTGGGATTTGGAGAAGATGAATTAGGA GAGGTTTACATTCTATCAAGCAGTAAGAGTATGACCCAGACTCACAATGGAAAACTCTACAAGATCGTAG ACCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGTCACAGTTCAACCTGCCCAGCCACTGACCTCCGA TTGCTCCCGGCTCTGTCGAAACGGCTACTACACCCCCACTGGCAAGTGCTGCTGCAGTCCCGGCTGGGAG GGAGACTTCTGCAGAATTGCCAAGTGTGAGCCAGCGTGCCGTCATGGAGGTGTCTGTGTCAGACCGAACA AGTGCCTCTGTAAAAAGGGCTATCTTGGTCCTCAATGTGAACAAGTGGACAGGAACGTCCGCAGAGTGAC CAGGGCAGGTATCCTTGATCAGATCATTGACATGACGTCTTACTTGCTGGATCTCACAAGTTACATTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210126 protein sequence
Red=Cloning site Green=Tags(s) MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRVMSQLELLSGGEILCG GFYPRVSCCLQSDSPGLGRLENKIFSATNNSECSRLLEEIQCAPCSPHSQSLFYTPERDVLDGDLALPLL CKDYCKEFFYTCRGHIPGLLQTTADEFCFYYARKDAGLCFPDFPRKQVRGPASNYLGQMEDYEKVGGISR KHKHNCLCVQEVMSGLRQPVSAVHSGDGSHRLFILEKEGYVKILTPEGELFKEPYLDIHKLVQSGIKGGD ERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDVRTARVFLEVAELHR KHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQ PPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGRGYESEPSLLEFKPFSNGP LVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGASSSCRGYFSGHILGFGEDELG EVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRVTVQPAQPLTSDCSRLCRNGYYTPTGKCCCSPGWE GDFCRIAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNVRRVTRAGILDQIIDMTSYLLDLTSYIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020259 |
ORF Size | 2103 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020259.1, NM_020259.2, NM_020259.3, NM_020259.4, NP_064655.3 |
RefSeq Size | 9094 bp |
RefSeq ORF | 2103 bp |
Locus ID | 15245 |
MW | 78.5 kDa |
Gene Summary | Modulates hedgehog signaling in several cell types, including brain and lung through direct interaction with members of the hedgehog family. Soluble forms inhibit Shh-induced differentiation in the fibroblast cell line C3H/10T1/2. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203592 | Hhip (untagged) - Mouse Hedgehog-interacting protein (Hhip), (10ug) |
USD 210.00 |
|
MG210126 | Hhip (GFP-tagged) - Mouse Hedgehog-interacting protein (Hhip) |
USD 670.00 |
|
MR210126L3 | Lenti ORF clone of Hhip (Myc-DDK-tagged) - Mouse Hedgehog-interacting protein (Hhip) |
USD 810.00 |
|
MR210126L4 | Lenti ORF clone of Hhip (mGFP-tagged) - Mouse Hedgehog-interacting protein (Hhip) |
USD 810.00 |
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