Hhip (NM_020259) Mouse Tagged ORF Clone

CAT#: MR210126

Hhip (Myc-DDK-tagged) - Mouse Hedgehog-interacting protein (Hhip)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hhip
• Synonyms: Hhip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210126 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGATGCTCTCGTTTAAGCTGCTACTGCTGGCCGTGGCTCTGGGCTTCTTTGAAGGAGATGCGA
  AGTTTGGGGAAAGGAACGAGGGGAGCGGAGCGAGAAGGAGACGGTGCCTGAATGGGAACCCCCCAAAGCG
  CCTAAAGAGAAGGGACAGGCGGGTGATGTCCCAGCTGGAGCTGCTCAGTGGAGGAGAGATCCTGTGTGGT
  GGCTTCTACCCACGAGTATCTTGCTGCCTGCAGAGTGACAGCCCTGGATTGGGGCGTCTGGAGAACAAGA
  TCTTTTCTGCCACCAACAACTCAGAATGCAGCAGGCTGCTGGAGGAGATCCAATGTGCTCCCTGCTCCCC
  GCATTCCCAGAGCCTCTTCTACACACCTGAAAGAGATGTCCTGGATGGGGACCTAGCACTTCCACTCCTC
  TGCAAAGACTACTGCAAAGAATTCTTTTATACTTGCCGAGGCCATATTCCAGGTCTTCTTCAAACAACTG
  CTGATGAATTTTGCTTTTACTATGCAAGAAAAGATGCTGGGTTATGCTTTCCAGACTTCCCGAGAAAGCA
  AGTCAGAGGACCAGCATCTAACTACTTGGGCCAGATGGAAGACTACGAGAAAGTGGGGGGGATCAGCAGA
  AAACACAAACACAACTGCCTCTGTGTCCAGGAGGTCATGAGTGGGCTGCGGCAGCCTGTGAGCGCTGTGC
  ACAGCGGGGATGGCTCCCATCGGCTCTTCATTCTAGAGAAGGAAGGCTACGTGAAGATTCTAACCCCAGA
  AGGAGAACTGTTCAAGGAGCCTTACTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAC
  GAAAGGGGCCTGCTAAGCCTGGCATTCCATCCCAATTACAAGAAAAATGGAAAGCTGTATGTGTCTTATA
  CCACCAACCAGGAACGGTGGGCTATTGGGCCTCACGACCACATTCTTCGGGTTGTGGAATACACAGTATC
  CAGGAAAAACCCCCATCAAGTTGATGTGAGAACAGCCAGGGTGTTTCTGGAAGTCGCAGAGCTCCACCGA
  AAGCATCTTGGGGGACAGCTGCTCTTTGGTCCTGATGGCTTTTTGTACATCATCCTTGGGGATGGTATGA
  TCACATTGGATGACATGGAAGAGATGGATGGGTTAAGTGACTTCACAGGCTCTGTGCTGAGGCTGGACGT
  GGACACCGACATGTGCAATGTGCCTTATTCCATACCTCGGAGTAACCCTCACTTCAACAGCACCAACCAG
  CCCCCAGAAGTATTTGCCCACGGCCTCCATGATCCAGGCAGATGTGCCGTGGATCGACATCCTACTGATA
  TAAACATCAATTTAACAATACTTTGCTCAGATTCCAACGGGAAAAACAGGTCATCAGCCAGAATCCTACA
  GATAATAAAGGGAAGAGGTTATGAAAGTGAGCCATCTCTTCTTGAATTCAAGCCATTCAGTAACGGCCCT
  TTGGTTGGTGGATTTGTTTACAGAGGCTGTCAGTCTGAAAGATTGTACGGAAGCTATGTGTTCGGAGATC
  GCAATGGGAATTTCTTAACCCTCCAGCAAAGCCCAGTGACCAAGCAATGGCAAGAAAAGCCGCTCTGCCT
  GGGTGCCAGCAGCTCCTGTCGAGGCTACTTTTCGGGTCACATCTTGGGATTTGGAGAAGATGAATTAGGA
  GAGGTTTACATTCTATCAAGCAGTAAGAGTATGACCCAGACTCACAATGGAAAACTCTACAAGATCGTAG
  ACCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGTCACAGTTCAACCTGCCCAGCCACTGACCTCCGA
  TTGCTCCCGGCTCTGTCGAAACGGCTACTACACCCCCACTGGCAAGTGCTGCTGCAGTCCCGGCTGGGAG
  GGAGACTTCTGCAGAATTGCCAAGTGTGAGCCAGCGTGCCGTCATGGAGGTGTCTGTGTCAGACCGAACA
  AGTGCCTCTGTAAAAAGGGCTATCTTGGTCCTCAATGTGAACAAGTGGACAGGAACGTCCGCAGAGTGAC
  CAGGGCAGGTATCCTTGATCAGATCATTGACATGACGTCTTACTTGCTGGATCTCACAAGTTACATTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210126 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRVMSQLELLSGGEILCG
  GFYPRVSCCLQSDSPGLGRLENKIFSATNNSECSRLLEEIQCAPCSPHSQSLFYTPERDVLDGDLALPLL
  CKDYCKEFFYTCRGHIPGLLQTTADEFCFYYARKDAGLCFPDFPRKQVRGPASNYLGQMEDYEKVGGISR
  KHKHNCLCVQEVMSGLRQPVSAVHSGDGSHRLFILEKEGYVKILTPEGELFKEPYLDIHKLVQSGIKGGD
  ERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDVRTARVFLEVAELHR
  KHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQ
  PPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGRGYESEPSLLEFKPFSNGP
  LVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGASSSCRGYFSGHILGFGEDELG
  EVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRVTVQPAQPLTSDCSRLCRNGYYTPTGKCCCSPGWE
  GDFCRIAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNVRRVTRAGILDQIIDMTSYLLDLTSYIV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020259
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020259.1, NM_020259.2, NM_020259.3, NM_020259.4, NP_064655.3
• RefSeq Size: 9094 bp
• RefSeq ORF: 2103 bp
• Locus ID: 15245
• MW: 78.5 kDa
• Gene Summary: Modulates hedgehog signaling in several cell types, including brain and lung through direct interaction with members of the hedgehog family. Soluble forms inhibit Shh-induced differentiation in the fibroblast cell line C3H/10T1/2. [UniProtKB/Swiss-Prot Function]