Smg5 (BC100408) Mouse Tagged ORF Clone

CAT#: MR210133

Smg5 (Myc-DDK-tagged) - Mouse Smg-5 homolog, nonsense mediated mRNA decay factor (C

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smg5
• Synonyms: MGC30464, MGC56792, mKIAA1089
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210133 representing BC100408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAAGGCCCTCCTCCAGGGGAGAGCAGCGAACCCGAGGCAAAGGTCCTCCACACCAAGCGGCTTT
  ACCGGGCTGTGGTGGAGGCTGTGCATCGGCTAGATCTCATCCTTTGCAACAAAGCTGCTTATCAGGAAGT
  GTTCAAACCAGAGAACGTTAGCCTGAGGAACAAGCTACGAGAGCTCTGTGTGAAGCTTATGTTCTTGCAT
  CCAGTAGACTATGGGAGGAGGGCTGAGGAACTGCTGTGGAGAAAGGTATACTATGAAGTTATCCAACTTA
  TCAAGACTAACAAAAAGCACATCCACAGTCGGAGCACCTTGGAATGTGCCTACAGGACTCATCTGGTCGC
  TGGCATTGGCTTCTACCAGCATCTCCTTCTCTATATCCAGTCCCACTACCAGCTGGAACTACAGTGCTGC
  ATCGACTGGACTCACGTCACCGATCCCCTCATGGGATTCAAGAAGCCAGTATCTGCTTCAGGAAAGGAGA
  TGGATTGGGCACAAATGGCTTGCCACCGATGCCTGGTGTACCTGGGGGATCTGTCACGCTATCAGAATGA
  GTTGGCTGGTGTGGACACCGAGCTGCTAGCTGAGAGATTCTACTATCAAGCCTTGTCAGTGGCTCCCCAG
  ATTGGAATGCCCTTCAATCAGCTGGGTACCCTTGCAGGCAGCAAGTATTACAACGTGGAAGCCATGTATT
  GCTACTTGCGCTGCATCCAGTCGGAAGTGTCCTTTGAGGGAGCCTATGGGAACCTCAAGAGACTATATGA
  CAAGGCAGCCAAAATGTACCACCAGTTGAAGAAGTCTGAGACCAGGAAGCTCTCCCCTAGCAAAAAGCGA
  TGCAAAGACATTAAGAGGTTGTTGGTGAACTTCATGTACCTACAAAGCCTGTTACAGCCCAAAAGTAGCT
  CCGTGGACTCAGAACTGACCTCACTCTGCCAGTCAGTCCTAGAGGATTTCAACCTCTGCCTCTTCTACCT
  GCCCTCCTCACCCAACCTTGGTCTAACCAACGAGGATGAGGAGGAGTGTGAAAGTGGATATGCTTTCCTC
  CCCGACCTGCTCATCTTTCAGATGGCCATCATCTGCCTCATGGGTGTGCACAGTTTAAAGAGAGCAGGAT
  CCAAGCATTATAGTGCAGCCATTGCTTTCACCCTGGCCCTCTTTTCCCACCTTATCAATCATGTCAACAT
  ACGGCTGCAGGCGGAACTGGAAGAGGGCGAGAACCCTGTCTCAGCTTTTCAGAGTGATGGCACAGATGAA
  CCAGAGTCAAAAGAAGCACTAGAAAAAGAGGAGCCAGAGCCTGAGCCTCCCACTGTGGTACCCCAAGCTG
  ATGAGGGCAGAAAGAGCCGTAAGCACTCCCGACTCTCTTGTCTACGTCGTCGCCGCCGCCACCATCCTCC
  TAAAGCTGGTGATGACAGTGACCTGAGTGAGGGTTTTGAATCTGACTCTAGCCACGACTCTGCCCAGGCC
  AGTGACGGCTCAGACAGTGGCTCTGATAAGAGCCTGGAAGGCAGGGGCACTGCTTTTGATGCAGAGACAG
  ACTCAGAAATGAACAGCCAGGAGTCCCGGTCAGACCTGGAAGATATGGAGGATGAGGAGGGGACAAGATC
  TCCAGCCCAGGAGCCCCCTCAGGCCAGATCAGAGGTTCCAGATTCCCTCAATGGCCCCCTGGGCCCCAGT
  GAAGCAAGCATTGCCAGCAATTTACAAGCCATGTCCACCCAGATGTTCCAGACCAAGCGCTGCTTCCGAC
  TGGCTCCCACTTTCAGCAACCTGCTCCTGCAGCCCACCACGGAACCTAACAGTGTGGCCAGTCACAGGCC
  TTGTGTCAACGGGGATATGGACAAGCCTTTAGAACCAGGTGCGTGGGTGCATGAGAGTCCACCTTCTCGC
  AGAGTAACCTCACCCTTGGGGTTTTGCTGTCACTGTGGAATCCTTGTCCTGTGCACGTACTCATTTCCAG
  CCTCCAGAACGTTTGCTGTCTTGCTGCTTTCCACTGCAGACTGCTCCAGCTTCACTCTCTTCTCTCCTCA
  TATTGGAGGTTGTACTTCCTTCCCTCATGGCATCCAGCTTCCTGAAGGCTCCCAGAAGCTGGGCATAATG
  TTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210133 representing BC100408
  Red=Cloning site Green=Tags(s)
  
  MSQGPPPGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKAAYQEVFKPENVSLRNKLRELCVKLMFLH
  PVDYGRRAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCC
  IDWTHVTDPLMGFKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQ
  IGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKSETRKLSPSKKR
  CKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLGLTNEDEEECESGYAFL
  PDLLIFQMAIICLMGVHSLKRAGSKHYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVSAFQSDGTDE
  PESKEALEKEEPEPEPPTVVPQADEGRKSRKHSRLSCLRRRRRHHPPKAGDDSDLSEGFESDSSHDSAQA
  SDGSDSGSDKSLEGRGTAFDAETDSEMNSQESRSDLEDMEDEEGTRSPAQEPPQARSEVPDSLNGPLGPS
  EASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEPNSVASHRPCVNGDMDKPLEPGAWVHESPPSR
  RVTSPLGFCCHCGILVLCTYSFPASRTFAVLLLSTADCSSFTLFSPHIGGCTSFPHGIQLPEGSQKLGIM
  FL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC100408
• ORF Size: 2106 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC100408.1
• RefSeq Size: 3121
• RefSeq ORF: 2108
• Locus ID: 229512
• MW: 114.4 kDa