Irak1 (NM_001177973) Mouse Tagged ORF Clone

CAT#: MR210252

Irak1 (Myc-DDK-tagged) - Mouse interleukin-1 receptor-associated kinase 1 (Irak1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Irak1
• Synonyms: AA408924; Il1rak; IRAK; IRAK-1; IRAK1-S; IRAK1b; mPLK; Plpk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210252 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCGGCCTTGATCGTGCGCGACCAGACAGAGCTGC
  GGCTGTGCGAGCGCTCCGAGCAGCGCACAGCCAGTGTTCTGTGGCCCTGGATCAACCGCAACGCGCGCGT
  AGCTGACCTCGTTCACATCCTCACGCACCTGCAGCTGCTGCGTGCGAGGGACATCATCACAGCCTGGCAC
  CCTCCTGCCCCCGTTGTGCCCCCAAGCACCGCTGCCCCAAGGCCCAGCAGCATCTCTGCAGGCTCTGAGG
  CCGGGGACTGGAGCCCCCGGAAATTGCAGTCCTCTGCCTCCACCTTCCTCTCCCCAGCTTTTCCAGGCTC
  CCAGACCCATTCTGAGTCAGAGCTCCTCCAGGTTCCACTCCCTGTTTCCCTCGGGCCACCACTACCATCT
  TCAGCCCCTTCCTCCACCAAGCAGTCAAGCCCAGAGAGCCCAGTGTCTGGCCTCCAAAGAGCCCATCCCT
  CCCCGTTTTGCTGGCCCTTCTGTGAGATTTCCCAAGGCACCTGCAACTTCTCTGAAGAACTCAGGATTGG
  AGAGGGTGGTTTTGGATGTGTGTACCGAGCAGTCATGAGAAATACTACATATGCTGTGAAGAGACTGAAG
  GAGGAAGCTGACCTAGAGTGGACTATGGTGAAACAGAGCTTCTTAACAGAGGTGGAACAGCTATCAAGGT
  TTCGTCACCCAAATATCGTAGACTTTGCTGGCTACTGTGCAGAGAGTGGCTTATACTGCCTTGTTTATGG
  CTTCTTGCCCAATGGCTCCTTGGAGGATCAGCTCCACCTTCAGACCCAGGCCTGCTCCCCACTTTCCTGG
  CCTCAACGACTGGACATTCTTCTGGGCACAGCCCGGGCTATTCAGTTTTTACATCAGGATAGCCCCAGCC
  TTATCCATGGAGACATCAAGAGTTCTAACGTGCTTCTGGATGAGAGACTGATGCCCAAGCTGGGAGACTT
  TGGCCTGGCTCGTTTCAGCCGCTTTGCGGGGGCCAAAGCAAGCCAGAGCAGTACTGTGGCCCGGACTTCC
  ACAGTTCGAGGTACCCTCGCCTACTTGCCTGAGGAGTACATCAAGACAGGCCGACTGGCTGTGGACACCG
  ATACCTTCAGCTTTGGGGTGGTAATACTGGAGACCCTTGCTGGTCAGAGGGCTGTGAGGACACAAGGTGC
  AAAGACCAAGTATTTGAAAGACCTGATTGAAGATGAGGCTGAAGAGGCTGGAGTGACCCTGAAAAGCACC
  CAGCCTACTCTGTGGGTGGGTGTAGCCACGGATGCTTGGGCTGCTCCAATTGCTGCCCAGATCTATAAGA
  AGCACCTGGACTCCAGACCTGGGCCCTGCCCACCCCAGTTGGGCCTGGCCCTGGCTCAACTAGCTTGCTG
  CTGCATGCACCGTCGGGCCAAGAAGAGGCCCCCCATGACCCAGAGGCAAAACTCCCAACATTCCTTGCTT
  GCCCTTTGGCTCTATTTGGGGAGGTGCTCTGTGGAACTAAGTGGTAAAGGGCTGCTGCTTTGCTGGATGA
  GCCTCAGATCCCAGGAACAGGTATACAAGAGACTAGAAGGGCTTCAGGCAGGGCCTCCCTGGGAGCTAGA
  GGTTGCCGGCCATGGCTCCCCTTCCCCACAGGAGAACTCCTACATGTCTACCACTGGCAGTGCCCAGAGT
  GGGGATGAACCATGGCAGCCTCTAGTAGTGACCACAAGAGCCCCAGCCCAGGCTGCCCAGCAACTCCAGA
  GAAGTCCCAACCAGCCAGTGGAAAGTGATGAGAGTGTTCCCGGCCTCTCTGCTACCCTGCATTCCTGGCA
  CTTGACTCCAGGTTCCCACCCAAGCCCAGCGTCCTTCAGAGAGGCTAGCTGTACCCAGGGAGGCACTACC
  AGAGAATCAAGTGTGAGGAGTAGCCCAGGCTTCCAGCCTACAACCATGGAAGGCTCACCCACGGGCAGCT
  CATCCCTGCTGTCATCAGAACCACCACAGATCATCATCAACCCAGCCCGACAGAAGATGGTACAAAAGCT
  GGCTCTTTATGAAGAAGGGGTCTTGGATAGCCTGCAACTGCTGTCATCAGGCTTTTTCCCAGGCTTGGAT
  TTAGAACCTGAAAAGAGCCAGGGACCTGAAGAAAGTGATGAATTCCAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210252 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDALEPADWCQFAALIVRDQTELRLCERSEQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWH
  PPAPVVPPSTAAPRPSSISAGSEAGDWSPRKLQSSASTFLSPAFPGSQTHSESELLQVPLPVSLGPPLPS
  SAPSSTKQSSPESPVSGLQRAHPSPFCWPFCEISQGTCNFSEELRIGEGGFGCVYRAVMRNTTYAVKRLK
  EEADLEWTMVKQSFLTEVEQLSRFRHPNIVDFAGYCAESGLYCLVYGFLPNGSLEDQLHLQTQACSPLSW
  PQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLMPKLGDFGLARFSRFAGAKASQSSTVARTS
  TVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIEDEAEEAGVTLKST
  QPTLWVGVATDAWAAPIAAQIYKKHLDSRPGPCPPQLGLALAQLACCCMHRRAKKRPPMTQRQNSQHSLL
  ALWLYLGRCSVELSGKGLLLCWMSLRSQEQVYKRLEGLQAGPPWELEVAGHGSPSPQENSYMSTTGSAQS
  GDEPWQPLVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPGSHPSPASFREASCTQGGTT
  RESSVRSSPGFQPTTMEGSPTGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPGLD
  LEPEKSQGPEESDEFQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177973
• ORF Size: 2154 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3955 bp
• RefSeq ORF: 2253 bp
• Locus ID: 16179
• MW: 78.3 kDa