Ehhadh (NM_023737) Mouse Tagged ORF Clone

CAT#: MR210264

Ehhadh (Myc-DDK-tagged) - Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ehhadh
• Synonyms: 1300002P22Rik; HD; L-PBE; LBFP; LBP; MFP; MFP1; PBFE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210264 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGTATCTGAGGCTGCCCCACTCCCTGGCTATGATCCGCCTCTGCAATCCACCGGTCAATGCCA
  TCAGTCCAACTGTAATCACAGAAGTAAGGAATGGACTCCAGAAAGCTAGTTTGGACCATACGGTTAGAGC
  CATAGTGATCTGTGGAGCAAATGACAACTTCTGTGCAGGTGCTGATATCCATGGCTTTAAATCTCCCACT
  GGCCTTACATTAGGAAGCTTGGTAGATGAAATACAGCGATACCAGAAGCCAGTGGTGGCCGCCATCCAAG
  GCGTGGCTCTTGGAGGAGGACTAGAGCTGGCCTTGGGCTGTCACTATCGGATTGCCAATGCAAAGGCTCG
  TGTTGGCTTCCCGGAAGTGATGCTGGGAATTCTTCCTGGTGCAAGAGGAACGCAGCTTCTACCCAGGGTC
  GTTGGAGTTCCTGTTGCTCTTGACTTAATTACCTCAGGAAGACATATTTCAACAGATGAAGCACTCAAGC
  TTGGAATTCTGGATGTAGTTGTAAAGTCAGACCCAGTTGAAGAAGCCATCAAATTTGCTCAGACGGTTAT
  AGGTAAACCCATAGAACCCCGCAGGATCCTAAACAAGCCAGTCCCAAGCTTGCCCAACATGGACAGTGTT
  TTTGCAGAAGCCATTGCCAAGGTACGGAAGCAGTACCCTGGCCGCCTGGCTCCGGAGACTTGTGTCCGTT
  CAGTCCAGGCCTCCGTGAAGCATCCATATGAAGTGGCCATCAAGGAAGAAGCAAAGCTGTTTATGTACCT
  TCGGGGGTCGGGGCAGGCTAGAGCCCTGCAGTACGCCTTTTTTGCTGAAAAGTCTGCAAATAAGTGGTCA
  ACTCCCTCAGGAGCATCTTGGAAAACAGCATCTGCTCAACCCGTCTCCTCGGTTGGTGTTCTTGGCTTGG
  GAACGATGGGCCGAGGCATCGCCATCTCTTTTGCAAGGGTGGGGATCCCTGTGGTTGCTGTAGAGTCAGA
  CCCAAAGCAGCTAGATACTGCAAAGAAGATAATAACTTCCACCTTGGAAAAGGAAGCATCGAAGAGTGGC
  CAAGCTTCAGCAAAACCAAACCTCAGGTTCTCCTCATCCACAAAGGAGCTTTCGAGTGTGGATTTAGTGA
  TTGAAGCAGTGTTCGAAGATATGAACCTGAAGAAGAAGGTCTTCGCTGAACTGTCAGCCCTGTGCAAGCC
  GGGAGCCTTTCTGTGCACCAATACCTCAGCACTGGATGTGGATGACATTGCTTCTTCCACAGATCGCCCC
  CAGCTGGTGATTGGCACCCACTTCTTCTCCCCAGCCCACATCATGAGGTTACTAGAGGTCATTCCTAGCC
  GATACTCTTCCCCCACTACCATCGCCACAGTCATGAGCTTATCCAAAAGGATTGGAAAGATTGGAGTCGT
  TGTTGGTAACTGCTATGGATTTGTTGGGAATCGAATGTTGGCTCCCTATTACAACCAGGGCTATTTCTTG
  ATAGAGGAAGGTAGTAAGCCAGAGGATGTAGATGGGGTCTTAGAAGAGTTTGGTTTTAGAATGGGACCCT
  TCAGGGTGTCTGACCTCGCAGGGCTAGATGTGGGTTGGAAAGTTCGCAAAGGGCAAGGCCTTACTGGACC
  GTCCTTACCTCCAGGAACCCCCACCCGAAAGAGGGGCAATACCAGGTACTCCCCAATTGCTGATATGCTC
  TGTGAAGCTGGGCGATTTGGTCAGAAGACAGGTAAGGGCTGGTATCAGTATGACAAGCCACTGGGTCGCA
  TCCACAAACCTGATCCCTGGCTTTCTGAGTTTCTGTCACAGTATAGAGAAACCCATCACATCAAGCAGCG
  CTCCATCAGCAAGGAGGAAATCCTGGAGCGTTGCTTATATTCCCTTATCAACGAGGCATTCCGCATCTTG
  GAGGAGGGGATGGCCGCTAGCCCAGAGCACATTGATGTCATCTACTTGCATGGGTATGGGTGGCCAAGGC
  ACGTGGGTGGGCCCATGTACTATGCTGCCTCAGTTGGGCTGCCCACAGTTCTAGAGAAATTGCAGAAATA
  TTACAGACAGAATCCTGACATCCCCCAGCTGGAGCCCAGTGACTACCTGAGGAGGCTGGTTGCCCAGGGA
  AGCCCTCCTCTGAAAGAATGGCAAAGCTTGGCAGGACCCCATAGCAGCAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210264 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEYLRLPHSLAMIRLCNPPVNAISPTVITEVRNGLQKASLDHTVRAIVICGANDNFCAGADIHGFKSPT
  GLTLGSLVDEIQRYQKPVVAAIQGVALGGGLELALGCHYRIANAKARVGFPEVMLGILPGARGTQLLPRV
  VGVPVALDLITSGRHISTDEALKLGILDVVVKSDPVEEAIKFAQTVIGKPIEPRRILNKPVPSLPNMDSV
  FAEAIAKVRKQYPGRLAPETCVRSVQASVKHPYEVAIKEEAKLFMYLRGSGQARALQYAFFAEKSANKWS
  TPSGASWKTASAQPVSSVGVLGLGTMGRGIAISFARVGIPVVAVESDPKQLDTAKKIITSTLEKEASKSG
  QASAKPNLRFSSSTKELSSVDLVIEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALDVDDIASSTDRP
  QLVIGTHFFSPAHIMRLLEVIPSRYSSPTTIATVMSLSKRIGKIGVVVGNCYGFVGNRMLAPYYNQGYFL
  IEEGSKPEDVDGVLEEFGFRMGPFRVSDLAGLDVGWKVRKGQGLTGPSLPPGTPTRKRGNTRYSPIADML
  CEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSEFLSQYRETHHIKQRSISKEEILERCLYSLINEAFRIL
  EEGMAASPEHIDVIYLHGYGWPRHVGGPMYYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRLVAQG
  SPPLKEWQSLAGPHSSKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023737
• ORF Size: 2157 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023737.2, NM_023737.3, NP_076226.2
• RefSeq Size: 3010 bp
• RefSeq ORF: 2157 bp
• Locus ID: 74147
• MW: 78.3 kDa