Mark2 (NM_001080388) Mouse Tagged ORF Clone

CAT#: MR210289

Mark2 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 2 (Mark2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mark2
• Synonyms: Emk; EMK-1; Par-1; Par-1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210289 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCGCTCGGACCCCCCTACCCACGCTGAACGAAAGGGACACGGAGCAGCCCACCTTGGGACACC
  TTGATTCCAAGCCCAGCAGTAAGTCCAACATGCTGCGGGGCCGCAACTCAGCCACCTCTGCTGACGAGCA
  GCCCCATATTGGCAACTACCGGCTCCTTAAGACCATTGGCAAGGGTAACTTTGCCAAGGTGAAGTTGGCC
  CGGCACATCCTGACGGGGAAAGAGGTAGCTGTGAAGATCATCGACAAGACCCAGCTGAACTCCTCCAGCC
  TACAGAAACTGTTCCGAGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTTAAGTTGTTTGA
  AGTGATCGAGACTGAGAAGACTCTCTACCTTGTCATGGAGTATGCCAGTGGCGGAGAGGTGTTTGATTAC
  CTAGTGGCCCATGGCAGGATGAAAGAAAAAGAAGCTCGAGCCAAATTTCGCCAGATAGTGTCTGCTGTGC
  AGTACTGTCACCAGAAGTTCATTGTTCATAGAGATCTAAAGGCAGAAAACCTGCTCCTGGATGCTGATAT
  GAACATCAAGATTGCAGACTTTGGCTTTAGCAACGAATTCACCTTTGGGAACAAGCTGGATACTTTCTGT
  GGCAGTCCTCCTTATGCTGCCCCAGAACTTTTCCAGGGCAAAAAGTATGATGGTCCTGAGGTGGATGTCT
  GGAGCCTGGGAGTCATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATGGACAGAACCTCAAGGA
  GCTGCGGGAACGGGTACTGAGGGGGAAATACCGTATTCCGTTCTACATGTCCACGGACTGTGAAAATCTG
  CTTAAGAAATTTCTCATACTTAATCCTAGTAAGAGAGGCACTTTAGAGCAAATTATGAAAGATCGGTGGA
  TGAACGTGGGGCATGAGGACGATGAGCTAAAGCCTTATGTGGAACCTCTCCCTGACTACAAGGACCCCCG
  GCGGACAGAGTTGATGGTGTCAATGGGTTACACACGGGAAGAGATCCAGGACTCGCTGGTAGGCCAGAGG
  TACAACGAAGTGATGGCTACCTATCTGCTCCTTGGCTACAAGAGCTCTGAGCTGGAAGGTGATACCATCA
  CTTTGAAGCCCCGGCCTTCAGCTGATCTAACCAACAGCAGTGCCCCATCTCCATCCCACAAGGTTCAGCG
  CAGCGTCTCTGCCAACCCCAAGCAACGACGCTCCAGTGACCAGGCCGTCCCTGCCATTCCCACCTCGAAT
  TCCTACTCTAAGAAGACTCAGAGTAACAACGCAGAAAATAAGCGGCCTGAGGAAGAGACAGGGCGGAAAG
  CCAGCAGCACCGCCAAAGTGCCTGCCAGCCCTCTGCCTGGCCTGGACAGGAAGAAGACCACTCCTGCCCC
  CTCCACGAACAGCGTCCTTTCCACCAGCACAAACCGAAGCAGGAACTCCCCACTTTTGGACAGGGCCAGC
  CTTGGCCAGGCCTCCATCCAGAATGGTAAAGACAGCACAGCCCCCCAGCGCGTCCCTGTCGCCTCCCCCT
  CCGCCCACAACATCAGCAGCAGTAGTGGAGCCCCAGACCGAACTAATTTCCCACGGGGTGTGTCCAGTCG
  AAGCACCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAGCAGAATCTACCCTACGGTGTGACCCCA
  GCCTCTCCCTCTGGCCATAGCCAGGGCCGGCGGGGGGCCTCTGGCAGCATCTTCAGCAAGTTCACCTCCA
  AGTTTGTCCGCAGGAACCTGAATGAACCTGAAAGCAAAGACCGAGTGGAGACGCTCAGACCTCACGTGGT
  AGGCAGTGGAGGCACTGACAAGGACAAGGAGGAGTTTCGGGAGGCCAAGCCTCGCTCCCTGCGCTTCACC
  TGGAGCATGAAGACCACGAGCTCTATGGAGCCCAATGAGATGATGCGGGAGATCCGCAAGGTGCTGGACG
  CCAACAGCTGCCAAAGCGAGCTGCACGAGCGGTACATGCTACTGTGCGTGCATGGCACACCAGGCCACGA
  GAACTTTGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCCCGGCTGTCTCTCAACGGTGTTCGGTTTAAG
  CGGATATCGGGCACTTCCATGGCCTTCAAAAACATTGCCTCCAAAATAGCCAATGAGCTGAAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210289 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMLRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLA
  RHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDY
  LVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFC
  GSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENL
  LKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQR
  YNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRSSDQAVPAIPTSN
  SYSKKTQSNNAENKRPEEETGRKASSTAKVPASPLPGLDRKKTTPAPSTNSVLSTSTNRSRNSPLLDRAS
  LGQASIQNGKDSTAPQRVPVASPSAHNISSSSGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTP
  ASPSGHSQGRRGASGSIFSKFTSKFVRRNLNEPESKDRVETLRPHVVGSGGTDKDKEEFREAKPRSLRFT
  WSMKTTSSMEPNEMMREIRKVLDANSCQSELHERYMLLCVHGTPGHENFVQWEMEVCKLPRLSLNGVRFK
  RISGTSMAFKNIASKIANELKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080388
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080388.1, NM_001080388.2, NP_001073857.1
• RefSeq Size: 4502 bp
• RefSeq ORF: 2169 bp
• Locus ID: 13728
• MW: 80.9 kDa