Dgka (NM_016811) Mouse Tagged ORF Clone

CAT#: MR210336

Dgka (Myc-DDK-tagged) - Mouse diacylglycerol kinase, alpha (Dgka)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgka
• Synonyms: 80kDa; AW146112; Dagk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210336 representing NM_016811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAAGAGAAGGGCCTCATCAGCCCAGAAGACTTCGCTCAGCTGCAAAAGTACATAGAATACTCCA
  CCAAAAGGGTCAGCGATGTACTGAAGGTCTTTGACGATGGTGAGATGAACAGATTTTGCCAGGGAGATGC
  CATTGGGTACCTAGGATTTGAACAATTCATGAAAATGTATCTGGAAATGGAGGAGGTTCCCCATCACCTA
  TGCTGGGCTCTGTTTTGGTCCTTCCATACTAGTCAAGTTGCGGCTGAGAAGACCAAGTCAAAAGCCAATG
  TGATCTGTCTCAGTGATGTGTACTGCTACTTCACCCTCCTAGAAGGTGGCCGGCCAGAAGACAAGCTAGA
  GTTCACCTTCAAGCTATATGACATGGACAGAAATGGGATCCTGGATAGCACGGAAGTGGAAAAAATCATC
  CTTCAGATGATGCGAGTGGCCGAATATCTAGACTGGGATGTGTCTGAGCTGAGACCGATCCTTCAGGAGA
  TGATGAGAGAGATGGATCAGGACGGCAGTGGCTCTGTGTCTCTAGACGAGTGGGTCCGTGCTGGGGCTAC
  CACAGTGCCACTGCTTGTGCTCCTGGGGATGGACGTGACTATGAAAGATGATGGCAACCATATATGGAGA
  CCCAAGAGATTCACCAGACTGGTCTACTGCAACCTGTGCGAACAGAGCATTAGCCTTGGCAAACAGGGCC
  TGAGCTGTAACTTCTGTAAGTACATTGTTCATGACCACTGTGCCATGAAGGCCCAGCCTTGTGAAGTCAG
  CACCTATGCCAAGTCTCGGAAAGACATTGGTGTCCAGTCACACTTATGGGTTCGAGGAGGCTGTCATTCC
  GGGCGTTGTGACCGCTGCCAGAAAAAGATCCGGACCTACCACAGCCTAACAGGACTGCACTGTGTGTGGT
  GCCACCTGGAGATCCATGATGACTGTCTGCAGGCTGTAGGTCCTGAGTGTGACTGTGGGTTGCTCCGTGA
  TCATATCCTGCCTCCGTGTTCCATCTACCCCAGCGTCCTGGTATCTGGACAAGAGTGCAAACACAAGACC
  ACAGATGATACAAGCCTGTGCACCCCTGAGGCTTTTCGGATTGAACCCGTTTCTAACACCCACCCCCTTC
  TAGTCTTCATCAATCTTAAGAGCGGAGGCAAGCAGGGGCAGAGTGTGCTTTGGAAATTTCAGTACATTCT
  GAACCCGCGGCAGGTGTTCGACCTGAAGGATGGTCCGGAGCCAGGGCTCAGATTCTTTAAAGATGTTCCT
  CAGTTCCGGATATTGGTGTGTGGTGGAGATGGCACAGTAGGCTGGGTTCTAGAGACCATTGACAAAGCCA
  ACTTTGCCACTGTGCCTCCCGTTGCTGTGCTGCCCCTGGGCACTGGAAATGATCTGGCTCGGTGCCTAAG
  ATGGGGAAGAGGTTATGAAGGTGAGAATTTGAGAAAGATTCTCAAGGATATAGAGCTAAGTAAGGTGGTA
  TATCTCGACCGATGGTTCCTGGAAGTGATTCCCCAACAAAATGGAGAAAAGAGTGATCCAGTTCCCTCTC
  AAATCATCAATAACTACTTCTCCATTGGCGTGGATGCTTCTATCGCTCACCGATTCCATCTCATGAGAGA
  GAAATATCCTGAGAAGTTCAATAGCAGAATGAAGAACAAGCTTTGGTACTTTGAGTTTGCCACATCTGAG
  TCCATCTTCTCAACATGCAAAAAGCTGGAAGAGTCTGTAACCGTTGAGATATGCGGGAAGCTGCTGGATC
  TGAGCGACCTATCCCTAGAAGGCATTGCGGTATTGAACATCCCGAGCACGCACGGTGGCTCCAACCTCTG
  GGGTGACACCAAGAGACCTCATGGGGATACATGTGAGATCAACCAGGCATTGGGCAGTGCGGCCAAAATA
  ATCACAGACCCCGATATCCTGAAGACCTGTGTGCCAGACATGAGTGACAAGCGGCTGGAAGTGGTAGGAA
  TAGAGGGTGCAATTGAGATGGGCCAGATCTATACCAGGCTCAAGAGTGCTGGACACCGGCTGGCCAAGTG
  CTCCGAGATCACATTCCAGACCACAAAAACCCTCCCCATGCAAATTGACGGGGAGCCCTGGATGCAGGCA
  CCCTGTACAATCAAGATCACCCATAAGAACCAGATGCCTATGCTTATGGGTCCACCTTCCAACTCCTACA
  ATTTCTTTGGCTTTTGGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210336 representing NM_016811
  Red=Cloning site Green=Tags(s)
  
  MAKEKGLISPEDFAQLQKYIEYSTKRVSDVLKVFDDGEMNRFCQGDAIGYLGFEQFMKMYLEMEEVPHHL
  CWALFWSFHTSQVAAEKTKSKANVICLSDVYCYFTLLEGGRPEDKLEFTFKLYDMDRNGILDSTEVEKII
  LQMMRVAEYLDWDVSELRPILQEMMREMDQDGSGSVSLDEWVRAGATTVPLLVLLGMDVTMKDDGNHIWR
  PKRFTRLVYCNLCEQSISLGKQGLSCNFCKYIVHDHCAMKAQPCEVSTYAKSRKDIGVQSHLWVRGGCHS
  GRCDRCQKKIRTYHSLTGLHCVWCHLEIHDDCLQAVGPECDCGLLRDHILPPCSIYPSVLVSGQECKHKT
  TDDTSLCTPEAFRIEPVSNTHPLLVFINLKSGGKQGQSVLWKFQYILNPRQVFDLKDGPEPGLRFFKDVP
  QFRILVCGGDGTVGWVLETIDKANFATVPPVAVLPLGTGNDLARCLRWGRGYEGENLRKILKDIELSKVV
  YLDRWFLEVIPQQNGEKSDPVPSQIINNYFSIGVDASIAHRFHLMREKYPEKFNSRMKNKLWYFEFATSE
  SIFSTCKKLEESVTVEICGKLLDLSDLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDTCEINQALGSAAKI
  ITDPDILKTCVPDMSDKRLEVVGIEGAIEMGQIYTRLKSAGHRLAKCSEITFQTTKTLPMQIDGEPWMQA
  PCTIKITHKNQMPMLMGPPSNSYNFFGFWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016811
• ORF Size: 2190 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016811.1, NM_016811.2, NP_058091.2
• RefSeq Size: 2842
• RefSeq ORF: 2193
• Locus ID: 13139
• MW: 82.8 kDa