Naglu (NM_013792) Mouse Tagged ORF Clone

CAT#: MR210384

Naglu (Myc-DDK-tagged) - Mouse alpha-N-acetylglucosaminidase (Sanfilippo disease IIIB) (Naglu)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Naglu
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210384 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCCGCGGGGCTGGCCGTGATTCTGGGGTTCCTACTCCTGGCTGGGGGCTCTGTGGGTGATGAGG
  CTCGGGAGGCAAAGGCCGTGCGGGAACTGGTGGTCCGGCTACTGGGACCCGGGCCGGCGGCCAATTTCTT
  GGTGTCCGTGGAGCGCGCGCTGGCGGACGAGTCGGGCCTGGACACCTACAGCCTGAGCGGCGGCGGCGGG
  GTGCCAGTGCTTGTGCGCGGCTCCACGGGCGTGGCGGCAGCCGCGGGGCTGCACCGCTACCTGCGTGACT
  TCTGTGGCTGTCAGGTGGCCTGGTCCAGCGCTCAGCTGCACCTGCCGTGGCCGCTGCCCGCTGTGCCCGA
  CGGGCTGACCGAAACCACGCCCAACAGGTACCGCTATTACCAGAATGTGTGCACGCACAGCTACTCCTTC
  GTGTGGTGGGACTGGGCCCGTTGGGAGCAAGAGATTGACTGGATGGCACTGAATGGCATCAACCTGGCTC
  TGGCTTGGAATGGCCAGGAGGCCATCTGGCAAAGGGTATACCTGGCCTTGGGCCTGACCCAGTCAGAGAT
  CGATACGTACTTCACCGGTCCTGCCTTCCTGGCCTGGGGACGCATGGGTAACTTGCACACCTGGGATGGC
  CCCCTGCCCCGTTCCTGGCACCTCAGTCAAGTCTACCTGCAGCATCGAATCCTGGACCGGATGCGCTCCT
  TTGGCATGATCCCAGTGCTGCCTGCCTTCGCAGGGCATGTCCCCAAGGCCATCACCAGGGTGTTTCCACA
  GGTCAATGTCATCAAGTTGGGGAGCTGGGGACATTTCAACTGCTCCTACAGCTGCTCCTTCCTTCTGGCT
  CCAGGAGACCCCATGTTTCCCCTCATCGGGAACCTCTTCCTACGGGAGCTGACCAAGGAGTTTGGCACAG
  ATCATATCTATGGGGCTGACACCTTCAATGAGATGCAGCCTCCCTTCTCTGACCCCTCCTACCTCGCCGC
  TACCACTGCAGCCGTCTATGAAGCCATGGTCACTGTGGACCCTGATGCTGTTTGGCTGCTCCAAGGCTGG
  CTCTTCCAGCACCAGCCCCAGTTCTGGGGCCCCTCTCAAATCAGGGCTGTGCTGGAGGCCGTGCCCCGTG
  GTCGTCTCCTGGTTCTGGACCTGTTTGCTGAAAGCCATCCTGTTTACATGCACACAGCCTCCTTCCATGG
  CCAGCCCTTCATCTGGTGTATGCTCCACAACTTTGGGGGCAACCATGGCCTGTTTGGAGCCCTCGAGGAT
  GTGAACCGAGGCCCCCAGGCAGCTCGCCTCTTCCCTAACTCCACCATGGTCGGCACTGGCATAGCCCCCG
  AGGGCATTGGCCAGAATGAAGTGGTCTATGCTCTCATGGCTGAGCTGGGCTGGCGCAAGGACCCTGTACC
  AGATTTGATGGCCTGGGTGAGCAGCTTTGCCATCCGCCGATACGGGGTCTCCCAGCCTGATGCCGTGGCA
  GCTTGGAAGCTCCTACTCAGAAGTGTCTACAACTGCTCTGGGGAGGCGTGCAGTGGGCACAATCGAAGCC
  CGTTGGTCAAGCGGCCGTCCCTACAGATGAGTACCGCTGTCTGGTACAACAGATCAGATGTGTTTGAGGC
  TTGGCGACTGCTGTTAACAGCTGCCCCAAACCTGACCACCAGCCCAGCCTTCCGCTATGACCTGCTGGAT
  GTCACCCGCCAAGCCGTGCAGGAGTTGGTCAGCCTGTGCTATGAGGAGGCAAGGACCGCCTACCTGAAGC
  AAGAGCTTGATCTCCTGCTCAGGGCTGGAGGCCTCCTGGTCTATAAACTCCTGCCTACACTAGATGAGCT
  GCTGGCTAGCAGCAGCCACTTCTTGCTGGGTACCTGGTTGGATCAGGCCCGGAAAGCGGCCGTAAGTGAG
  GCCGAGGCCCAGTTCTATGAACAAAACAGCCGCTACCAGATTACCCTGTGGGGGCCCGAGGGCAACATTT
  TGGATTATGCCAATAAGCAACTGGCAGGACTGGTGGCTGATTACTACCAGCCTCGCTGGTGCCTCTTCTT
  GGGGACTCTGGCTCACAGCCTAGCCAGAGGTGTCCCCTTCCAACAGCACGAGTTTGAGAAGAACGTTTTC
  CCACTCGAGCAGGCTTTCGTTTACAACAAGAAGAGGTACCCCAGTCAGCCCCGAGGGGACACCGTGGACC
  TCTCCAAGAAGATCTTCCTCAAATATCACCCCCAGCCTGACTCTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210384 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEAAGLAVILGFLLLAGGSVGDEAREAKAVRELVVRLLGPGPAANFLVSVERALADESGLDTYSLSGGGG
  VPVLVRGSTGVAAAAGLHRYLRDFCGCQVAWSSAQLHLPWPLPAVPDGLTETTPNRYRYYQNVCTHSYSF
  VWWDWARWEQEIDWMALNGINLALAWNGQEAIWQRVYLALGLTQSEIDTYFTGPAFLAWGRMGNLHTWDG
  PLPRSWHLSQVYLQHRILDRMRSFGMIPVLPAFAGHVPKAITRVFPQVNVIKLGSWGHFNCSYSCSFLLA
  PGDPMFPLIGNLFLRELTKEFGTDHIYGADTFNEMQPPFSDPSYLAATTAAVYEAMVTVDPDAVWLLQGW
  LFQHQPQFWGPSQIRAVLEAVPRGRLLVLDLFAESHPVYMHTASFHGQPFIWCMLHNFGGNHGLFGALED
  VNRGPQAARLFPNSTMVGTGIAPEGIGQNEVVYALMAELGWRKDPVPDLMAWVSSFAIRRYGVSQPDAVA
  AWKLLLRSVYNCSGEACSGHNRSPLVKRPSLQMSTAVWYNRSDVFEAWRLLLTAAPNLTTSPAFRYDLLD
  VTRQAVQELVSLCYEEARTAYLKQELDLLLRAGGLLVYKLLPTLDELLASSSHFLLGTWLDQARKAAVSE
  AEAQFYEQNSRYQITLWGPEGNILDYANKQLAGLVADYYQPRWCLFLGTLAHSLARGVPFQQHEFEKNVF
  PLEQAFVYNKKRYPSQPRGDTVDLSKKIFLKYHPQPDSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013792
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013792.1, NM_013792.2, NP_038820.1
• RefSeq Size: 2504 bp
• RefSeq ORF: 2220 bp
• Locus ID: 27419
• MW: 82.6 kDa