Plod3 (NM_011962) Mouse Tagged ORF Clone
CAT#: MR210405
- TrueORF®
Plod3 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3)
"NM_011962" in other vectors (3)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Plod3 |
Synonyms | AI414586; LH3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210405 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGCAGGCCCGGAACCCCGGCTTTTGCTCCTGCTCCTGCTGCTGCTGCCGCCGCTGCCCCCCG TAACTTCTGCCTCCGATCGACCCCGGGGCGCCAATGCTGTCAACCCAGACAAATTGCTGGTGATCACTGT GGCTACGGCAGAGACAGAGGGGTACCGGCGTTTTCTGCAGTCTGCGGAGTTCTTTAACTACACTGTACGG ACCCTGGGCCTGGGACAGGAGTGGCGAGGGGGTGATGTGGCTCGAACAGTTGGTGGAGGCCAGAAGGTCA GGTGGCTCAAGAAGGAAATGGAGAAATATGCAGATCAGAAAGACATGATCATCATGTTTGTGGACAGCTA CGACGTGATTCTGGCAAGCAGCCCGACAGAGCTGCTGAAGAAGTTTGTTCAGAGTGGCAGTCATCTGCTG TTCTCTGCTGAGAGCTTCTGCTGGCCTGAGTGGGGGCTGGCAGAGCAGTACCCTGAGGTGGGCATGGGGA AGCGCTTCCTCAACTCTGGTGGATTCATCGGCTTTGCTCCCACCATCCATCAGATTGTCCGCCAGTGGAA CTACAAAGACGACGATGATGATCAATTGTTCTACACTCAACTGTACCTGGACCCAGGGCTGCGGGAGAAA CTCAAACTGAGTCTGGACCATAAATCCCGGATCTTTCAGAACCTCAATGGAGCCTTAGATGAAGTGATCT TAAAGTTTGACCAGAATCGTGTGCGCATCCGGAATGTGGCCTACGACACACTTCCTGTTGTGGTCCATGG AAATGGTCCCACTAAGCTCCAGCTCAACTACCTGGGGAACTATGTCCCCAATGGCTGGACTCCCCAGGGA GGCTGTGGATTCTGCAACCAGACCCTGAGGACACTCCCGGGGGGGCAGCCTCCCCCCCGGGTGCTTCTGG CCGTGTTTGTGGAGCAGCCTACTCCCTTCCTGCCTCGGTTCCTGCAGCGCCTACTGCTCCTGGATTACCC CCCGGACAGGATCTCTCTCTTCCTTCACAACAGCGAGGTGTACCACGAGCCTCATATTGCAGATGCCTGG CCACAGCTCCAGGACCATTTTTCAGCTGTAAAGCTGGTGGGGCCAGAGGAGGCCCTGAGCGCAGGGGAGG CCAGGGACATGGCCATGGACAGCTGTCGGCAGAACCCTGAGTGTGAGTTCTACTTTAGCCTGGATGCTGA TGCCGTCCTTACGAACCCGGAGACCTTGCGTGTCTTGATTGAACAAAACAGGAAGGTAATAGCTCCCATG CTTTCCCGCCATGGCAAGCTCTGGTCCAACTTCTGGGGTGCCCTGAGCCCCAATGAGTACTACGCCCGCT CCGAAGACTACGTGGAACTGGTGCAGCGGAAGCGAGTGGGCGTGTGGAATGTACCCTACATATCTCAGGC ATATGTGATCCGCGGGGAGACCCTGAGGACTGAACTGCCCCAAAAGGAGGTATTCTCCAGCAGCGACACA GACCCAGATATGGCTTTCTGCAAGAGCGTCCGGGACAAGGGCATCTTCCTCCACCTCAGCAACCAGCACG AGTTTGGCCGGCTGCTGGCTACTTCTCGCTATGACACAGACCACCTACACCCAGACCTCTGGCAGATCTT TGACAACCCTGTGGACTGGAGAGAACAGTACATTCACGAGAATTACAGCCGGGCCCTGGATGGGGAAGGG CTAGTGGAACAGCCATGCCCAGATGTGTACTGGTTCCCACTGCTGACCGAGCAGATGTGTGATGAGCTGG TGGAGGAAATGGAACACTATGGCCAGTGGTCTGGAGGCCGGCACGAGGATTCCAGGTTGGCTGGAGGATA CGAGAATGTTCCCACCGTTGATATCCACATGAAACAGGTGGGATACGAGGACCAGTGGCTTCAGCTGCTG CGGACATATGTGGGGCCCATGACGGAGTACCTGTTCCCTGGCTACCACACCAAGACACGGGCAGTGATGA ACTTCGTGGTCCGCTACCGGCCAGATGAGCAGCCCTCGCTTCGGCCACACCATGACTCGTCCACCTTCAC TCTCAATGTCGCCCTCAACCACAAGGGTGTAGATTATGAGGGAGGCGGCTGCCGCTTCCTGCGTTACGAC TGCAGAATCTCCTCTCCGAGGAAAGGCTGGGCCCTCCTGCACCCTGGCCGCCTCACACACTACCATGAGG GGCTGCCCACCACCCGGGGTACTCGATACATCATGGTGTCCTTTGTTGACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210405 protein sequence
Red=Cloning site Green=Tags(s) MAAAGPEPRLLLLLLLLLPPLPPVTSASDRPRGANAVNPDKLLVITVATAETEGYRRFLQSAEFFNYTVR TLGLGQEWRGGDVARTVGGGQKVRWLKKEMEKYADQKDMIIMFVDSYDVILASSPTELLKKFVQSGSHLL FSAESFCWPEWGLAEQYPEVGMGKRFLNSGGFIGFAPTIHQIVRQWNYKDDDDDQLFYTQLYLDPGLREK LKLSLDHKSRIFQNLNGALDEVILKFDQNRVRIRNVAYDTLPVVVHGNGPTKLQLNYLGNYVPNGWTPQG GCGFCNQTLRTLPGGQPPPRVLLAVFVEQPTPFLPRFLQRLLLLDYPPDRISLFLHNSEVYHEPHIADAW PQLQDHFSAVKLVGPEEALSAGEARDMAMDSCRQNPECEFYFSLDADAVLTNPETLRVLIEQNRKVIAPM LSRHGKLWSNFWGALSPNEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGETLRTELPQKEVFSSSDT DPDMAFCKSVRDKGIFLHLSNQHEFGRLLATSRYDTDHLHPDLWQIFDNPVDWREQYIHENYSRALDGEG LVEQPCPDVYWFPLLTEQMCDELVEEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLL RTYVGPMTEYLFPGYHTKTRAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGVDYEGGGCRFLRYD CRISSPRKGWALLHPGRLTHYHEGLPTTRGTRYIMVSFVDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011962 |
ORF Size | 2226 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011962.1, NM_011962.2, NM_011962.3, NP_036092.1 |
RefSeq Size | 3278 bp |
RefSeq ORF | 2226 bp |
Locus ID | 26433 |
MW | 84.9 kDa |
Gene Summary | Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG210405 | Plod3 (GFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) |
USD 750.00 |
|
MR210405L3 | Lenti ORF clone of Plod3 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) |
USD 880.00 |
|
MR210405L4 | Lenti ORF clone of Plod3 (mGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) |
USD 880.00 |
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