Plod3 (NM_011962) Mouse Tagged ORF Clone

CAT#: MR210405

Plod3 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plod3
• Synonyms: AI414586; LH3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210405 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCAGGCCCGGAACCCCGGCTTTTGCTCCTGCTCCTGCTGCTGCTGCCGCCGCTGCCCCCCG
  TAACTTCTGCCTCCGATCGACCCCGGGGCGCCAATGCTGTCAACCCAGACAAATTGCTGGTGATCACTGT
  GGCTACGGCAGAGACAGAGGGGTACCGGCGTTTTCTGCAGTCTGCGGAGTTCTTTAACTACACTGTACGG
  ACCCTGGGCCTGGGACAGGAGTGGCGAGGGGGTGATGTGGCTCGAACAGTTGGTGGAGGCCAGAAGGTCA
  GGTGGCTCAAGAAGGAAATGGAGAAATATGCAGATCAGAAAGACATGATCATCATGTTTGTGGACAGCTA
  CGACGTGATTCTGGCAAGCAGCCCGACAGAGCTGCTGAAGAAGTTTGTTCAGAGTGGCAGTCATCTGCTG
  TTCTCTGCTGAGAGCTTCTGCTGGCCTGAGTGGGGGCTGGCAGAGCAGTACCCTGAGGTGGGCATGGGGA
  AGCGCTTCCTCAACTCTGGTGGATTCATCGGCTTTGCTCCCACCATCCATCAGATTGTCCGCCAGTGGAA
  CTACAAAGACGACGATGATGATCAATTGTTCTACACTCAACTGTACCTGGACCCAGGGCTGCGGGAGAAA
  CTCAAACTGAGTCTGGACCATAAATCCCGGATCTTTCAGAACCTCAATGGAGCCTTAGATGAAGTGATCT
  TAAAGTTTGACCAGAATCGTGTGCGCATCCGGAATGTGGCCTACGACACACTTCCTGTTGTGGTCCATGG
  AAATGGTCCCACTAAGCTCCAGCTCAACTACCTGGGGAACTATGTCCCCAATGGCTGGACTCCCCAGGGA
  GGCTGTGGATTCTGCAACCAGACCCTGAGGACACTCCCGGGGGGGCAGCCTCCCCCCCGGGTGCTTCTGG
  CCGTGTTTGTGGAGCAGCCTACTCCCTTCCTGCCTCGGTTCCTGCAGCGCCTACTGCTCCTGGATTACCC
  CCCGGACAGGATCTCTCTCTTCCTTCACAACAGCGAGGTGTACCACGAGCCTCATATTGCAGATGCCTGG
  CCACAGCTCCAGGACCATTTTTCAGCTGTAAAGCTGGTGGGGCCAGAGGAGGCCCTGAGCGCAGGGGAGG
  CCAGGGACATGGCCATGGACAGCTGTCGGCAGAACCCTGAGTGTGAGTTCTACTTTAGCCTGGATGCTGA
  TGCCGTCCTTACGAACCCGGAGACCTTGCGTGTCTTGATTGAACAAAACAGGAAGGTAATAGCTCCCATG
  CTTTCCCGCCATGGCAAGCTCTGGTCCAACTTCTGGGGTGCCCTGAGCCCCAATGAGTACTACGCCCGCT
  CCGAAGACTACGTGGAACTGGTGCAGCGGAAGCGAGTGGGCGTGTGGAATGTACCCTACATATCTCAGGC
  ATATGTGATCCGCGGGGAGACCCTGAGGACTGAACTGCCCCAAAAGGAGGTATTCTCCAGCAGCGACACA
  GACCCAGATATGGCTTTCTGCAAGAGCGTCCGGGACAAGGGCATCTTCCTCCACCTCAGCAACCAGCACG
  AGTTTGGCCGGCTGCTGGCTACTTCTCGCTATGACACAGACCACCTACACCCAGACCTCTGGCAGATCTT
  TGACAACCCTGTGGACTGGAGAGAACAGTACATTCACGAGAATTACAGCCGGGCCCTGGATGGGGAAGGG
  CTAGTGGAACAGCCATGCCCAGATGTGTACTGGTTCCCACTGCTGACCGAGCAGATGTGTGATGAGCTGG
  TGGAGGAAATGGAACACTATGGCCAGTGGTCTGGAGGCCGGCACGAGGATTCCAGGTTGGCTGGAGGATA
  CGAGAATGTTCCCACCGTTGATATCCACATGAAACAGGTGGGATACGAGGACCAGTGGCTTCAGCTGCTG
  CGGACATATGTGGGGCCCATGACGGAGTACCTGTTCCCTGGCTACCACACCAAGACACGGGCAGTGATGA
  ACTTCGTGGTCCGCTACCGGCCAGATGAGCAGCCCTCGCTTCGGCCACACCATGACTCGTCCACCTTCAC
  TCTCAATGTCGCCCTCAACCACAAGGGTGTAGATTATGAGGGAGGCGGCTGCCGCTTCCTGCGTTACGAC
  TGCAGAATCTCCTCTCCGAGGAAAGGCTGGGCCCTCCTGCACCCTGGCCGCCTCACACACTACCATGAGG
  GGCTGCCCACCACCCGGGGTACTCGATACATCATGGTGTCCTTTGTTGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210405 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAGPEPRLLLLLLLLLPPLPPVTSASDRPRGANAVNPDKLLVITVATAETEGYRRFLQSAEFFNYTVR
  TLGLGQEWRGGDVARTVGGGQKVRWLKKEMEKYADQKDMIIMFVDSYDVILASSPTELLKKFVQSGSHLL
  FSAESFCWPEWGLAEQYPEVGMGKRFLNSGGFIGFAPTIHQIVRQWNYKDDDDDQLFYTQLYLDPGLREK
  LKLSLDHKSRIFQNLNGALDEVILKFDQNRVRIRNVAYDTLPVVVHGNGPTKLQLNYLGNYVPNGWTPQG
  GCGFCNQTLRTLPGGQPPPRVLLAVFVEQPTPFLPRFLQRLLLLDYPPDRISLFLHNSEVYHEPHIADAW
  PQLQDHFSAVKLVGPEEALSAGEARDMAMDSCRQNPECEFYFSLDADAVLTNPETLRVLIEQNRKVIAPM
  LSRHGKLWSNFWGALSPNEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGETLRTELPQKEVFSSSDT
  DPDMAFCKSVRDKGIFLHLSNQHEFGRLLATSRYDTDHLHPDLWQIFDNPVDWREQYIHENYSRALDGEG
  LVEQPCPDVYWFPLLTEQMCDELVEEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLL
  RTYVGPMTEYLFPGYHTKTRAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGVDYEGGGCRFLRYD
  CRISSPRKGWALLHPGRLTHYHEGLPTTRGTRYIMVSFVDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011962
• ORF Size: 2226 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011962.1, NM_011962.2, NM_011962.3, NP_036092.1
• RefSeq Size: 3278 bp
• RefSeq ORF: 2226 bp
• Locus ID: 26433
• MW: 84.9 kDa
• Gene Summary: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. [UniProtKB/Swiss-Prot Function]