Rfx3 (NM_011265) Mouse Tagged ORF Clone

CAT#: MR210451

Rfx3 (Myc-DDK-tagged) - Mouse regulatory factor X, 3 (influences HLA class II expression) (Rfx3), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rfx3
• Synonyms: C230093O12Rik; MRFX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210451 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACTTCAGAGACGGGTTCAGACACAGGTTCGACAGTGACTCTGCAGACGTCTGTGGCTAGCCAAG
  CAGCAGTGCCTACACAGGTGGTACAGCAAGTGCCAGTGCAGCAGCAGGTGCAGCAGGTACAGACAGTTCA
  GCAGGTCCAACATGTCTACCCAGCTCAGGTGCAGTATGTGGAAGGAAGTGATACTGTCTATACCAATGGA
  GCAATCCGAACAACAACTTATCCCTACACAGAAACACAGATGTACAGCCAAAACACTGGAGGAAATTACT
  TTGATACTCAAGGAAGTTCTGCCCAGGTGACAACTGTGGTGTCCTCCCACAGTATGGTGGGTACTGGTGG
  GATTCAGATGGGCGTCACAGGAGGACAACTCATCAGCAGCTCGGGAGGAACCTATTTGATCGGCAATTCA
  ATGGAGAACTCTGGTCACTCAGTGACACACACAACTCGGGCCTCCCCAGCAACAATTGAAATGGCGATTG
  AGACGCTGCAAAAGTCTGACGGTCTGTCCACTCACAGAAGCTCTCTTCTCAACAGCCATCTGCAATGGCT
  GCTGGACAATTATGAGACAGCGGAGGGAGTGAGCCTGCCGAGGAGCACGCTGTACAACCACTACCTTCGA
  CACTGCCAGGAGCACAAGCTGGACCCGGTCAATGCCGCCTCTTTCGGAAAACTAATAAGGTCCATTTTTA
  TGGGGCTACGAACTAGGAGATTAGGCACTAGAGGAAACTCCAAATACCATTACTATGGGATTCGTGTGAA
  GCCAGATTCTCCTCTTAACCGTCTACAAGAAGATATGCAGTACATGGCTATGAGGCAGCAGCCCATGCAA
  CAGAAGCAAAGGTACAAGCCTATGCAGAAAGTGGATGGGGTTGCAGATGGTTTCACAGGAAGTGGCCAAC
  AGACAGGCACATCTGTTGAGCAAACTGTAATTGCCCAAAGTCAACATCATCAACAGTTTTTAGATGCATC
  TCGAGCACTTCCAGAGTTTGGAGAAGTTGAAATCTCTTCTCTGCCAGATGGTACTACCTTTGAGGATATC
  AAGTCACTGCAGAGTCTTTATCGAGAGCACTGTGAGGCAATACTGGACGTTGTTGTGAATCTTCAGTTTA
  GCCTGATAGAAAAACTGTGGCAAACATTCTGGCGCTATTCCCCATCTACTCCAGCCGACGGCACTACCAT
  CACGGAATCAAGCAATCTGAGTGAAATAGAAAGTCGACTTCCGAAAGCAAAGCTGATAACTCTGTGCAAA
  CATGAGTCTATCCTGAAATGGATGTGTAACTGTGACCATGGGATGTACCAGGCTTTGGTGGAGATTCTCA
  TCCCCGACGTCCTTAGACCCATTCCTAGTGCCTTGACCCAAGCCATTCGAAATTTCGCAAAAAGCCTAGA
  AGGTTGGCTTTCAAATGCCATGAACAATATTCCACAGAGAATGATTCAAACCAAGGTTGCCGCTGTAAGT
  GCCTTTGCTCAGACTCTGCGAAGATACACATCGCTCAATCACCTGGCCCAGGCAGCCCGCGCAGTGCTTC
  AGAACACTTCCCAGATCAACCAGATGCTCAGTGACCTCAACCGCGTTGACTTTGCCAATGTCCAGGAGCA
  GGCTTCCTGGGTGTGCCAGTGTGATGACAACATGGTTCAGAGACTAGAAACAGACTTCAAGATGACCCTG
  CAGCAGCAGAGCACTCTGGAGCAGTGGGCTGCTTGGCTGGACAATGTGATGATGCAAGCGCTGAAGCCCT
  ATGAAGGAAGGCCCAGCTTCCCGAAGGCTGCTCGGCAGTTTCTGCTCAAGTGGTCTTTCTACAGCTCCAT
  GGTTATCCGGGACTTAACCTTGCGCAGTGCTGCTAGCTTTGGCTCCTTTCACCTGATCCGGCTGCTCTAT
  GATGAGTACATGTTCTACTTAGTAGAGCATCGTGTTGCTCAAGTGACAGGAGAGACACCGATAGCAGTGA
  TGGGTGAGTTTGGCGATTTAAATGCTGTATCTCCCGGAAATCTGGATAAAGATGAAGGCAGCGAAGTCGA
  AAGTGAGACGGATGAAGATCTGGATGATTCTTCAGAGCCTCGGGCCAAAAGGGAGAAAACGGAGCTGAGC
  CAGGCGTTCCCAGTAGGCTGCATGCAGCCTGTTCTGGAGAGTGCTGTGCAGCCCAGCCTCCTGAACCCCC
  TACACAGCGAGCACATCGTCACAAGTACACAGACCATCAGGCAGTGCAGCGCCACAGGCAACACCTACAC
  TGCAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210451 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQTSETGSDTGSTVTLQTSVASQAAVPTQVVQQVPVQQQVQQVQTVQQVQHVYPAQVQYVEGSDTVYTNG
  AIRTTTYPYTETQMYSQNTGGNYFDTQGSSAQVTTVVSSHSMVGTGGIQMGVTGGQLISSSGGTYLIGNS
  MENSGHSVTHTTRASPATIEMAIETLQKSDGLSTHRSSLLNSHLQWLLDNYETAEGVSLPRSTLYNHYLR
  HCQEHKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMAMRQQPMQ
  QKQRYKPMQKVDGVADGFTGSGQQTGTSVEQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGTTFEDI
  KSLQSLYREHCEAILDVVVNLQFSLIEKLWQTFWRYSPSTPADGTTITESSNLSEIESRLPKAKLITLCK
  HESILKWMCNCDHGMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLSNAMNNIPQRMIQTKVAAVS
  AFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCDDNMVQRLETDFKMTL
  QQQSTLEQWAAWLDNVMMQALKPYEGRPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLY
  DEYMFYLVEHRVAQVTGETPIAVMGEFGDLNAVSPGNLDKDEGSEVESETDEDLDDSSEPRAKREKTELS
  QAFPVGCMQPVLESAVQPSLLNPLHSEHIVTSTQTIRQCSATGNTYTAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011265
• ORF Size: 2250 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011265.1, NM_011265.2, NM_011265.3, NP_035395.2
• RefSeq Size: 9187 bp
• RefSeq ORF: 2250 bp
• Locus ID: 19726
• MW: 83.5 kDa