Zfp458 (NM_001001152) Mouse Tagged ORF Clone

CAT#: MR210473

Zfp458 (Myc-DDK-tagged) - Mouse zinc finger protein 458 (Zfp458)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp458
• Synonyms: BC062958; mszf59-2; Rslcan-7; Znf728
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210473 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACATGTTGTCATTCTGGGATGTGGCTATATATTTCTCTGCAGAGGAGTGGGAATGTCTGGGTC
  CTGCTCAGTGGAAACTGTACAGGGACGTGATGCTGGAGAATTACAGCAACCTTGTGTTCCTGGGTCTTGC
  TTCTTCTAAGCCATACCTGGTCACATTTCTGGAACAAATACAAGAGCCTTCAGATGTGAAGAGACAAGCG
  GCCATCACTGTGCATCCAGGGGGAAAATGCTATACATGCAAAGAATGTGGCAAAGGCTTTGAGCACAAAA
  AAGTATATCAGAATCACCGGAGAATTCATTTAAGAGTGAAGTCCTACAAGTGTGAAGAATGTGGCAAGTC
  CTTCCGTTTTCCATCATTACTTTCTGCACATAAAAGAAATCATACAGGACAAAAACCCTACAACTGTGAA
  ATATGTAGTAAAGCCTTCCACGTTCCTTCATTGCTTTCTGTACACAAGAGAATTCATAGAGTACAGAAAC
  CCTACAAGTCTGAAGATTATGGCAAGACCTTACATTGTCCATCATTACTTTCTCAACACAAGATAGTTCA
  TACAGGAGAAAAACCCTACCAGTGTGAAGACTTGGGCAAGGCCTTCCGTTATCCATCAAGACTTTCCAAC
  CATAAGAAAATTCATACAGGAGAGAAACCACACAAGTGTGAAGTATGTGGCAAGGCCTTCGATTATCCAT
  CAAGACTTTCCAACCATAAGAGAATTCATACAGGAGAGAAACCCTACAAATGTGAAGTATGTGGCAAAGC
  CTTCCATGATCCATCAAAACTCTCTCAACACAAAATAATTCATACAGGAGAAAAACCCTACAAATGTGAA
  GTTTGTGGCAAGACCTTCCATTATCCATCCATACTTTCTAAACATAAAATAATTCATACAAAAGAGAATC
  TCTGCAAGTGTGATATATGTGGCAAGGCCTTCCATTATCCATTATTACTTTCTCAACACAAAATTGTTCA
  CACAGGAGAAAAACCCTACAAATGTGAAGACTGTGGCAAGGCCTTCTATTATCCATCAAGACTTTCCAGA
  CATAAGAAAATACATACAGGAGAGAAACCATACAAGTGTGAAGTATGTGGCAAGGCCTTCTATTATCTAT
  CCATACTTTCTAAACACATGATAGTTCATACAGAAGAAAATCCTTACAAGTGTGAAGTATGTGGCAAGGC
  CTTTGATTATCCATCAAGGCTTTCCAATCATAAGAAAATTCATACAGAAGATAAACCACACAAGTGCGAA
  GTATGTGGAAATGCCTTCTGTTTTTCATCATCACTTCGTAAACACAAGATAATTCACACAGGAGAAAAAC
  CCTACAAGTGTGACATATGTGGCAAGGCCTTTGGTTCTCCATCAAGACTTTCCAAACATAACAAAATTCA
  TACAGAAGATAAACCATACAAGTGTGAAGTATGTGGAAAGGCCTTCCACTTTCCATCATTACTCTTGGTA
  CACAAGAGAATTCATACTGGAGAAAAACCTTACAAGTGTGAAGTATGTGGCAAGGCCTTCTATTGTCTAT
  CTACACTTTCTGTACACAAGAGAATTCATACTGGAGAATATGCCTACAAATGTGAAGTGTGTGGCAAAGC
  CTTCCATTGTCTATCAACATTGTCAGTGCACAAGAGAATTCATACTCAAGAAAAACCCTACAAGTGTGAA
  GTATGTGGCCAGGCATTCCATGCTTCATCAAAACTTTCTCATCACAAGAGAATTCATTCAGGAGAAAAAC
  CCCACAAATGTGAAATTTGTGGCAAATCATTCTATTATCCATCAAGACTTTCCAAGCATAAGGTAATTCA
  TACAGGAGAGAAACCGTACCACAGTCAAGCAAGTGGAAAGACCCTTGATTATCCTTCAAGACATTCCAAA
  CCTAAGACAATTAAGACAGGAGAAAAACCAAACAAATGTGAAATATGTGGAAAAGCCTTCTCCTTTCCAT
  CATTACTTTCTATACACAAGAGAATTCATATTGGAGAAAAACCCTATAATTGTGAAGTATGTGGCAAGGC
  TTTCCGAAATCCATCAAGACTTTCAAACCATAAGAAAATTCATACAGGAGAGAAACCATACAAGTGTGAA
  ATTTGTGGTAAGGCCTTTGATTATCCATCTTTACTTTCTAAACACAAGATCATTCATACAGGAGAGAAGC
  CCTACCACAGTGAAGTGTGTGGCAAGGCCTTCAGTTATTCATCAAGAATTTCCAAAGATAAGAAAATTTG
  TACAGGAGAGAAACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210473 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDMLSFWDVAIYFSAEEWECLGPAQWKLYRDVMLENYSNLVFLGLASSKPYLVTFLEQIQEPSDVKRQA
  AITVHPGGKCYTCKECGKGFEHKKVYQNHRRIHLRVKSYKCEECGKSFRFPSLLSAHKRNHTGQKPYNCE
  ICSKAFHVPSLLSVHKRIHRVQKPYKSEDYGKTLHCPSLLSQHKIVHTGEKPYQCEDLGKAFRYPSRLSN
  HKKIHTGEKPHKCEVCGKAFDYPSRLSNHKRIHTGEKPYKCEVCGKAFHDPSKLSQHKIIHTGEKPYKCE
  VCGKTFHYPSILSKHKIIHTKENLCKCDICGKAFHYPLLLSQHKIVHTGEKPYKCEDCGKAFYYPSRLSR
  HKKIHTGEKPYKCEVCGKAFYYLSILSKHMIVHTEENPYKCEVCGKAFDYPSRLSNHKKIHTEDKPHKCE
  VCGNAFCFSSSLRKHKIIHTGEKPYKCDICGKAFGSPSRLSKHNKIHTEDKPYKCEVCGKAFHFPSLLLV
  HKRIHTGEKPYKCEVCGKAFYCLSTLSVHKRIHTGEYAYKCEVCGKAFHCLSTLSVHKRIHTQEKPYKCE
  VCGQAFHASSKLSHHKRIHSGEKPHKCEICGKSFYYPSRLSKHKVIHTGEKPYHSQASGKTLDYPSRHSK
  PKTIKTGEKPNKCEICGKAFSFPSLLSIHKRIHIGEKPYNCEVCGKAFRNPSRLSNHKKIHTGEKPYKCE
  ICGKAFDYPSLLSKHKIIHTGEKPYHSEVCGKAFSYSSRISKDKKICTGEKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001152
• ORF Size: 2259 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001152.1, NM_001001152.2, NP_001001152.1
• RefSeq Size: 3488 bp
• RefSeq ORF: 2259 bp
• Locus ID: 238690
• Cytogenetics: 13 34.54 cM
• MW: 86.4 kDa