Exoc3 (NM_177333) Mouse Tagged ORF Clone

CAT#: MR210485

Exoc3 (Myc-DDK-tagged) - Mouse exocyst complex component 3 (Exoc3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Exoc3
• Synonyms: 2810050O03Rik; E430013E20Rik; Sec6; Sec6l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210485 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCAAAGATTCTGCCTGCTTTTTGACAATGAAGGAGACAGACCTGGAGGCTGTTGCAACAGCAGTCC
  AAAGGGTTGCTGGGATGCTTCAGCGCCCAGACCAACTGGACAAAGTGGAGCAGTATCGCAGAAGGGAAGC
  TCGGAAGAAGGCCTCTGTAGAGGCCAGGCTAAAGGCTGCAATCCAGTCTCAACTAGATGGGGTACGCACA
  GGCTTAAGCCAACTGCACAATGCACTGAATGACGTCAAGGATATCCAGCAGTCACTGGCTGATGTCAGCA
  AGGACTGGAGGCAAAGCATCAACACCATTGAGAGTCTCAAAGATGTAAAAGATGCAGTGGTGCAGCATAG
  CCAGCTGGCTGCAGCTGTGGAGAACCTCAAGAACATATTCTCAGTGCCTGAGATTGTGAGGGAGACCCAA
  GATCTCATCGAGCAAGGGGCCCTCCTGCAAGCCCACCGGAAGCTAATGGATCTGGAGTGCTCTCGGGATG
  GGCTAATGTGTGAGCAGTACCGCATGGACAGTGGGAACAAACGGGACATGACCCTCATTCATGGCTACTT
  TGGTAGCACACAGGGGCTCTCTGATGAGCTGGCTAAGCAGCTGTGGATGGTGCTGCAAAGATCACTGGTC
  ACTGTCCGCCGGGATCCTACCTTGCTGGTCTCCGTGGTCAGAATCATTGAAAGGGAAGAAAAAATTGACA
  GGCGGATACTTGATCGAAAAAAGCAAACTGGGTTTGTTCCTCCTGGAAGGCCCAAAAACTGGAAAGAAAA
  AATGTTTGCCATCTTGGACAGAACTGTGACAACTAGAATCGAAGGTACACAGGCAGACACCAGAGAATCT
  GACAAGATGTGGCTTGTGCGCCACCTGGAGATCATTAGGAAGTATGTCCTGGATGATCTCATTGTTGCCA
  AGAACCTGATGGTCCAGTGCTTCCCTCCTCACTATGAGATCTTTAAGAACCTCCTGAGCATGTACCACCA
  GGCCTTGAGCACTCGGATGCAGGACCTTGCATCAGAGGACCTTGAGGCCAACGAGATTGTGAGCCTCTTG
  ACATGGGTCCTAAATACCTACACCAGTGCAGAGATGATGGGGAATGTGGAACTAGCCCCAGAAGTGGATG
  TTAGTGCCCTGGAGCCCCTCCTCTCTCCAAACATCGTCTCCGAGCTGCTTGACACATACATGTCAACACT
  CACGTCCAACATCATTGCCTGGCTTCGGAAAGCACTGGAAACAGACAAGAAAGACTGGAGCAAAGAGACA
  GAGCCAGAAGCAGACCAGGATGGTTACTATCAGACTACACTTCCTGCCATTGTATTCCAGATGTTCGAAC
  AGAATCTTCAAGTTGCTGCTCAAATAAGTGAAGATTTGAAAACAAAGGTATTAGTGTTGTGTCTTCAGCA
  AATGAATTCTTTCCTAAGTAGATACAAAGATGAAGCCCAGCTTTACAAAGAAGAGCACCTGAGAAACCGG
  CAACACCCACACTGTTATGTGCAGTACATGATTGCCATCATCAATAACTGCCAGACCTTCAAAGAATCCA
  TCATCAGTCTGAAAAGGAAGTACCTAAAAACTGAAGCAGAGGAGGGCCTGTGTCTGAGTCAGCCAAGCAT
  GGATGGGATTCTAGACGCCATTGCTAAGGAAGGCTGCAGCAGTCTGCTAGAGGAGGTCTTCCTGGATCTA
  GAGCAACATCTGAATGAGCTGATGACAAAGAAGTGGTTGTTAGGGTCAAACGCTGTGGACATCATCTGTG
  TCACTGTAGAAGACTACTTCAATGACTTTGCCAAAATTAAAAAGCCATATAAAAAGAGAATGACAGCTGA
  GGCACACAGACGTGTGGTGGTGGAATACCTGCGGGCTGTCATGCAGAAGCGTATTTCCTTTCGGAGTGCT
  GAAGAGCGCAAGGAGGGCGCCGAGAAGATGGTCAGAGAGGCTGAACAGCTCCGCTTCCTGTTCCGGAAGC
  TGGCGTCTGGATTTGGTGAGGATGCAGATGGACACTGTGATACAATTGTTGCAGTGGCAGAGGTCATTAA
  GCTCACAGACCCTTCTCTGCTCTATCTAGAAGTCTCAACTCTGGTCAGCAAATATCCAGACATCAGAGAT
  GACCACATTGGTGCACTGCTGGCACTGCGAGGAGATGCCAGCCGGGACATGAAACAGACCATCATGGAGA
  CGCTGGAGCAGGGCCCCATGCAAGCAAGCCCCAACTATGTACCCATCTTCAAGGAGATTGTTGTGCCCAG
  TCTGAACGTGGCAAAGCTCCTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210485 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCKDSACFLTMKETDLEAVATAVQRVAGMLQRPDQLDKVEQYRRREARKKASVEARLKAAIQSQLDGVRT
  GLSQLHNALNDVKDIQQSLADVSKDWRQSINTIESLKDVKDAVVQHSQLAAAVENLKNIFSVPEIVRETQ
  DLIEQGALLQAHRKLMDLECSRDGLMCEQYRMDSGNKRDMTLIHGYFGSTQGLSDELAKQLWMVLQRSLV
  TVRRDPTLLVSVVRIIEREEKIDRRILDRKKQTGFVPPGRPKNWKEKMFAILDRTVTTRIEGTQADTRES
  DKMWLVRHLEIIRKYVLDDLIVAKNLMVQCFPPHYEIFKNLLSMYHQALSTRMQDLASEDLEANEIVSLL
  TWVLNTYTSAEMMGNVELAPEVDVSALEPLLSPNIVSELLDTYMSTLTSNIIAWLRKALETDKKDWSKET
  EPEADQDGYYQTTLPAIVFQMFEQNLQVAAQISEDLKTKVLVLCLQQMNSFLSRYKDEAQLYKEEHLRNR
  QHPHCYVQYMIAIINNCQTFKESIISLKRKYLKTEAEEGLCLSQPSMDGILDAIAKEGCSSLLEEVFLDL
  EQHLNELMTKKWLLGSNAVDIICVTVEDYFNDFAKIKKPYKKRMTAEAHRRVVVEYLRAVMQKRISFRSA
  EERKEGAEKMVREAEQLRFLFRKLASGFGEDADGHCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRD
  DHIGALLALRGDASRDMKQTIMETLEQGPMQASPNYVPIFKEIVVPSLNVAKLLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177333
• ORF Size: 2268 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177333.1, NM_177333.2, NM_177333.3, NP_796307.2
• RefSeq Size: 4597 bp
• RefSeq ORF: 2268 bp
• Locus ID: 211446
• MW: 86.5 kDa