Dcbld2 (NM_028523) Mouse Tagged ORF Clone

CAT#: MR210553

Dcbld2 (Myc-DDK-tagged) - Mouse discoidin, CUB and LCCL domain containing 2 (Dcbld2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcbld2
• Synonyms: 1700055P21Rik; AW146002; CLCP1; Esdn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210553 representing NM_028523
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCCGGGCGCCGCTGAGAGCCGCGCGCAGCCCCCAGGGTCCCGGAGGCCCGGCCGCGCCCGCCG
  CCACCGGCCGGGCCGCGCTGCCCAGCGCCGGCTGCTGTCCCCTCCCTCCTGGCCGCAACTCCTCCTCCAG
  GCCTCGACTGCTCCTTCTGCTGCTCCTACTGCTCCAGGACGCTGGAGGCCAGCAAGGTGATGGATGTGGA
  CACACTGTACTAGGCCCTGAGAGTGGAACCCTTACATCCATCAACTACCCACATACCTATCCTAACAGCA
  CTGTGTGTGAATGGGAGATTCGAGTCAGGACGGGAGAAAGGATTCGCATCAAATTCGGTGACTTTGACAT
  TGAAGATTCTGATTATTGTCACCTTAATTACCTGAAAATCTTTAATGGAATTGGAGTCAGCAGAACGGAA
  ATAGGCAAATACTGTGGTCTGGGTTTACAAATGAATCAGTCAATTGAGTCCAAAGGCAGTGAAGTCACAG
  TGCTGTTCATGAGTGGAACCCATGCTGCTGGGCGAGGATTTTTGGCTTCATACTCAGTTATAGATAAAGA
  AGATTTAATCACTTGTTTGGATACTGTATCTAATTTTTTGGAACCAGAGTTCAGTAAGTACTGCCCAGCT
  GGCTGTCTTTTGCCTTTTGCTGAAATATCTGGAACAATTCCTCATGGATACAGAGATTCTTCACCATTGT
  GTATGGCTGGAATCCATGCAGGAGTAGTGTCAAACGTGCTGGGTGGCCAAATCAGCATTGTGATTAGCAA
  AGGGACCCCATATTATGAAAGCTCTTTGGCCAACAATGTCACTTCCACGGTGGGATACTTATCTGCAAGT
  CTGTTTACATTTAAGACAAGTGGTTGCTATGGGACTCTGGGGATGGAGTCTGGTGTGATTGCCGATCCCC
  AGATAACAGCATCGTCTGCACTGGAGTGGACTGACCACATGGGGCAGGAGAACAGCTGGACAGCGGAGAA
  GGCCAGGCTGAGAAAACCCGGGCCTCCCTGGGCTGCTTTTGCCACTGATGAGCATCAGTGGCTGCAGATA
  GACCTTAACAAGGAGAAGAAGATAACAGGTATCGTAACCACTGGGTCTACCATGATAGAACACAGTTACT
  ATGTGTCTGCCTACAGAGTCCTGTACAGTGACGATGGGCAGAGATGGACTGTGTACAGAGAACCTGGTGT
  GGACCAGGACAAGATATTTCAAGGAAACAAAGATTATCACAAGGATGTTCGTAATAACTTTTTGCCACCA
  ATTATTGCACGTTTCATTAGAGTGAACCCTGTCCAGTGGCAACAGAAAATTGCCATGAAAGTGGAACTGC
  TCGGATGTCAGTTTACTCTCAAAGGTCGCCTTCCAAAGCTTACTCCACCTCCTCGGAACGGCAATAACCT
  CAGAAATACTACAGCTCGTCCCAAACTAGGTCGTGCCCCTAAATTTACTCAAGTGCTCCAACCTCGAAGT
  AGGAATGAACTTCCTGTGCAGCCGGCGGAGACAACTACCACTCCTGATATAAAAAACACGACTGTAACTC
  CCAGTGTAACCAAAGATGTCGCACTGGCTGCCGTTCTGGTCCCTGTGCTGGTCATGGCCCTCACCACACT
  CATCCTCATTCTAGTGTGTGCTTGGCACTGGAGAAACAGGAAGAAGAAAACTGAAGGCGCCTATGATTTA
  CCCCACTGGGATCGGGCAGGTTGGTGGAAAGGAATGAAGCAGCTTCTCCCTGCCAAGTCGGTGGACCACG
  AGGAGACGCCAGTGCGCTACAGCACTAGTGAAGTCAGTCACCTGAGTGCCAGGGAAGTCACCACAGTGCT
  GCAGGCCGACTCTGCAGAATATGCACAGCCCCTCGTGGGAGGAATTGTTGGCACACTCCATCAGAGATCC
  ACCTTTAAACCTGAGGAAGGGAAGGAAGCAGGCTATGCAGACCTCGATCCTTACAACTCTCCAATGCAGG
  AAGTGTACCACGCCTATGCTGAACCACTGCCCGTAACGGGGCCTGAGTACGCAACCCCGATCGTCATGGA
  CATGTCAGGGCACCCCACAGCCTCAGTTGGTCTGCCCTCCACATCCACCTTCAAAACTGCAGGGACCCAG
  CCTCACGCTTTAGTGGGAACTTACAACACTCTTCTCTCCAGGACTGACAGCTGTTCCTCAGGCCAGGCTC
  AGTATGACACCCCAAAAGGTGGGAAGTCAGCTGCTACCCCAGAGGAACTGGTATACCAGGTGCCCCAGAG
  CACCCAGGAGCTATCAGGAGCAGGAAGGGATGAGAAGTTTGATGCTTTTAAAGAAATCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210553 representing NM_028523
  Red=Cloning site Green=Tags(s)
  
  MASRAPLRAARSPQGPGGPAAPAATGRAALPSAGCCPLPPGRNSSSRPRLLLLLLLLLQDAGGQQGDGCG
  HTVLGPESGTLTSINYPHTYPNSTVCEWEIRVRTGERIRIKFGDFDIEDSDYCHLNYLKIFNGIGVSRTE
  IGKYCGLGLQMNQSIESKGSEVTVLFMSGTHAAGRGFLASYSVIDKEDLITCLDTVSNFLEPEFSKYCPA
  GCLLPFAEISGTIPHGYRDSSPLCMAGIHAGVVSNVLGGQISIVISKGTPYYESSLANNVTSTVGYLSAS
  LFTFKTSGCYGTLGMESGVIADPQITASSALEWTDHMGQENSWTAEKARLRKPGPPWAAFATDEHQWLQI
  DLNKEKKITGIVTTGSTMIEHSYYVSAYRVLYSDDGQRWTVYREPGVDQDKIFQGNKDYHKDVRNNFLPP
  IIARFIRVNPVQWQQKIAMKVELLGCQFTLKGRLPKLTPPPRNGNNLRNTTARPKLGRAPKFTQVLQPRS
  RNELPVQPAETTTTPDIKNTTVTPSVTKDVALAAVLVPVLVMALTTLILILVCAWHWRNRKKKTEGAYDL
  PHWDRAGWWKGMKQLLPAKSVDHEETPVRYSTSEVSHLSAREVTTVLQADSAEYAQPLVGGIVGTLHQRS
  TFKPEEGKEAGYADLDPYNSPMQEVYHAYAEPLPVTGPEYATPIVMDMSGHPTASVGLPSTSTFKTAGTQ
  PHALVGTYNTLLSRTDSCSSGQAQYDTPKGGKSAATPEELVYQVPQSTQELSGAGRDEKFDAFKEIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028523
• ORF Size: 2301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028523.2, NM_028523.3, NP_082799.2
• RefSeq Size: 6487 bp
• RefSeq ORF: 2310 bp
• Locus ID: 73379
• MW: 84.2 kDa