C6 (NM_016704) Mouse Tagged ORF Clone

CAT#: MR210555

C6 (Myc-DDK-tagged) - Mouse complement component 6 (C6)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C6
• Synonyms: AW111623
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210555 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGACATCTCACCTTGTGTTTCATTTTGCTGGTCATGCTGATTGACAAGAGTGAAGCCTGTTTCT
  GTGACCACTACCCATGGACTCACTGGTCCAGCTGTTCTAAGTCCTGCAATTCTGGAACCCAGAGCAGACA
  GAGACAAGTAGTAGTGAACGATTACTATTGGAAAAACTTATGCGATAAGCTTTGTATCAAGCAGGAGACC
  AGAGAGTGCAACTTGCAGACATGTCCCATCAACTGTGTCCTAGGAGACTATGGGACATGGTCAGACTGTG
  ACCCTTGTACTGAAAAACAGGTTAAAGTTAAATCTGTCCTGCGCCCAAGTCAGTTTGGGGGGCAACCATG
  CACAGAGCCCCTGGTGACCTTTCAACCATGTGTCCCCTCCAAGCTCTGCAAAATTGAAGAGACTAACTGC
  AAGAATAAGTTCCTCTGTGACAGTGGGCGCTGTATTCCCAGCAAGTTAGAATGCAATGGAGAGAATGACT
  GTGGAGACAATTCAGATGAAAGGAACTGTGGGAGGACAAAGCCAGTGTGCACACGGATATACACTCCCAT
  CCCTAGTGTGCAGCTGATGGGCACTGGGTTTCATTTTCTGGCAGGAGAGCCCAGAGGAGAAGTTCTTGAC
  AACTCTTTCACTGGAGGAATATGTAAACTTGTCAAGACCAGTCGAGCCAGTAATCCATACCGTGTTTCAG
  CCAATCTGGAAAATGTCAACTTTGAGGTACAAACTATAGAAGATGACTTGAAAACAGAATTCTACAAGAA
  TTTAATCTCTTTTGAAAAAAATAAAAATGAAGACAGTCTTTCAGTGGATGAAAGGACAAAATTTTTCCCT
  ATACCAATTTTCCATTTCTCAGAGAAAAATGAACATTCCCATTATAGCTCTGCCTTCAACAAAGTCATTA
  AAGCTTCCCACAAGAAGGATTCTAGCTTTATCAGGATCCATAAGCTGATAAAAGTCTTAAACTTCACAAT
  GAAAGCAACAGACCTACAGCTTTCAGATGTCTTCCTGAAAGCCCTTGTCCACCTGCCTTTAGAATACAAC
  TCTGCTGTGTACAGCCGGATATTTGATGACTTCGGGACCCACTACTTCACCTCAGGCTCCCTTGGGGGCA
  AGTATGACCTTATCTACCAATTCAGCCGCCAGGAGCTACAGAACTCAGGTTTAACAGAAGAAGAAGCTCA
  AAACTGTGTCCAGTATGAAACAAAGAAACTTAAGTTTCTTTATATGGAAATACACAAGGAAGACACGTGC
  ACCAAAAACAAGCTGTCTGAAAAATATGGAGGTTCCTTTTTGCAGGGATCAGAGAAATCCATTTCCCTGG
  TCCAGGGCGGGAGGAGTCAGCAGGCAGCAGCCTTGGCTTGGGAGAAGGGCACCTCTGGTCCAGAGGAGAA
  TGTCTATTCTGAGTGGCTAGAATCGGTGAAGGAAAACCCTGCTGTAGTTGATTATAAGCTTGCCCCAATT
  ACAGACTTGGTCAGAAACATCCCATGTGCAGTGACAAAACGGAACAACCTCAGGAGAGCGCTTCAAGAGT
  ATGCAGCCAAGTTTGACCCTTGCCAGTGTGCTCCATGTCCCAATAATGGCCGCCCCAGGCTCTCAGGCAC
  GGAATGCCTGTGTGTGTGCCAGAGCGGCACCTACGGTGAGAACTGTGAGCGCAGGTCCCCAGGTTACAAA
  TCTGATGCAGTGGATGGAAACTGGGGCTGCTGGTCTTCCTGGAGTGCGTGCAATGCTGCTTATAGGAGAT
  CAAGAACCCGAGAGTGTAATAACCCTGCGCCACAGCGAGGAGGACAAAGCTGTGGTGGCAAGGATCAGCA
  AGAAGAAGACTGTACAGTCTCAATAATGGAAAATGTTGGACAACCATGTATCAATGATGATGAAGAAATG
  AAAGAAGTAGACCTTGCTGAGCCAGAAGCAGAATCAGGGTGTTCTCAACCACCTCTCCCAGAAAACGCAT
  TTACCTGGAATGAGAAGAAACTGTACTCAGTTGGGGAGGAAGTTGAAATTTCATGTCTTACTGGATTCAC
  AGCTGTTGGATTCCAGTACTTGAGATGTTTACCAGACAGAACCTGGAGTCAAGGAGATGTGGAATGCCAA
  AGGACCTCGTGCCTCAAGCCCGTTGTTCAGGATGTCCTGACCATCTCCCCATTTCAGAGAGTGTATCAGA
  TTGGGGAATCCATTGAGCTGACATGCCCCAGAGGCTTTGTTGTTGCTGGACCATCAAGGTATACATGCAA
  GGAAGACTCCTGGACACCTCCCATTTCAAATTCATTGACCTGTGAACAAGGTGTCAGAGACCATCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210555 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTRHLTLCFILLVMLIDKSEACFCDHYPWTHWSSCSKSCNSGTQSRQRQVVVNDYYWKNLCDKLCIKQET
  RECNLQTCPINCVLGDYGTWSDCDPCTEKQVKVKSVLRPSQFGGQPCTEPLVTFQPCVPSKLCKIEETNC
  KNKFLCDSGRCIPSKLECNGENDCGDNSDERNCGRTKPVCTRIYTPIPSVQLMGTGFHFLAGEPRGEVLD
  NSFTGGICKLVKTSRASNPYRVSANLENVNFEVQTIEDDLKTEFYKNLISFEKNKNEDSLSVDERTKFFP
  IPIFHFSEKNEHSHYSSAFNKVIKASHKKDSSFIRIHKLIKVLNFTMKATDLQLSDVFLKALVHLPLEYN
  SAVYSRIFDDFGTHYFTSGSLGGKYDLIYQFSRQELQNSGLTEEEAQNCVQYETKKLKFLYMEIHKEDTC
  TKNKLSEKYGGSFLQGSEKSISLVQGGRSQQAAALAWEKGTSGPEENVYSEWLESVKENPAVVDYKLAPI
  TDLVRNIPCAVTKRNNLRRALQEYAAKFDPCQCAPCPNNGRPRLSGTECLCVCQSGTYGENCERRSPGYK
  SDAVDGNWGCWSSWSACNAAYRRSRTRECNNPAPQRGGQSCGGKDQQEEDCTVSIMENVGQPCINDDEEM
  KEVDLAEPEAESGCSQPPLPENAFTWNEKKLYSVGEEVEISCLTGFTAVGFQYLRCLPDRTWSQGDVECQ
  RTSCLKPVVQDVLTISPFQRVYQIGESIELTCPRGFVVAGPSRYTCKEDSWTPPISNSLTCEQGVRDHP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016704
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016704.1, NM_016704.2, NP_057913.1
• RefSeq Size: 2863 bp
• RefSeq ORF: 2310 bp
• Locus ID: 12274
• MW: 86.6 kDa