Brsk1 (NM_001003920) Mouse Tagged ORF Clone

CAT#: MR210598

Brsk1 (Myc-DDK-tagged) - Mouse BR serine/threonine kinase 1 (Brsk1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brsk1
• Synonyms: Gm1100; SAD-B; SADB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210598 representing NM_001003920
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCGGGTCCAAGGAAGGTGGCGGGGGCTCCCCCGCCTACCACCTCCCACACCCACACCCACACC
  CACCCCAGCACGCCCAATATGTGGGCCCCTATCGCCTGGAGAAGACGCTGGGCAAAGGACAGACAGGTCT
  AGTTAAACTTGGAGTCCACTGCATCACGGGTCAGAAGGTCGCTGTCAAGATCGTGAACAGGGAGAAGCTG
  TCGGAATCGGTGCTGATGAAGGTGGAGAGGGAAATCGCCATCCTGAAGCTCATTGAACACCCGCACGTGC
  TCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTATACTTGGTTCTTGAGCACGTTTCTGGTGGTGA
  GCTGTTCGACTACCTGGTAAAAAAAGGGAGACTGACACCCAAGGAGGCCCGGAAGTTCTTCCGCCAGATC
  GTGTCAGCACTGGACTTCTGCCATAGCTACTCCATCTGTCACAGAGACTTGAAGCCAGAGAACCTGCTGT
  TGGATGAGAAAAACAACATCCGCATCGCAGACTTTGGTATGGCGTCCCTGCAAGTGGGGGACAGCCTCCT
  GGAGACCAGCTGCGGGTCCCCCCATTACGCATGTCCAGAGGTGATCAAGGGGGAAAAGTATGATGGCCGC
  CGGGCAGACATGTGGAGCTGTGGAGTCATCCTATTTGCCCTGCTTGTGGGGGCACTGCCCTTCGATGACG
  ACAACCTGCGCCAGCTACTGGAGAAGGTGAAACGTGGGGTCTTCCACATGCCTCACTTCATCCCTCCAGA
  CTGCCAGAGCCTCCTGAGAGGGATGATTGAAGTGGAGCCCGAGAAAAGGCTCAGTCTGGAGCAAATTCAG
  AAACATCCTTGGTATCTGGGCGGGAAACACGAACCAGACCCTTGCCTGGAGCCAGCCCCAGGCCGCAGAG
  TAGCCATGCGTAGCCTGCCTTCCAATGGCGAGCTGGACCCTGACGTTCTGGAAAGCATGGCGTCTCTGGG
  CTGCTTCAGAGACCGCGAGCGGCTGCACAGAGAACTGCGAAGCGAGGAGGAAAACCAAGAAAAGATGATC
  TATTATTTGCTTTTGGATCGGAAAGAGCGGTATCCTAGCTGTGAAGACCAGGACCTGCCTCCTCGGAATG
  ATGTTGACCCACCTCGTAAGCGTGTGGATTCCCCCATGCTGAGCAGACACGGGAAACGGAGGCCAGAGCG
  GAAGTCCATGGAAGTCCTCAGTATCACAGATGCTGGGAGTGGTGGCTCCCCAGTGCCTACCAGACGGGCC
  TTGGAGATGGCCCAGCACAGTCAGAGATCCCGCAGCGTCAGTGGAGCCTCCACTGGTCTGTCCTCCAGCC
  CTCTGAGCAGCCCAAGGAGTCCGGTCTTTTCCTTCTCACCGGAGCCAGGCGCTGGAGATGAGGCTAGAGG
  CGGAGGCTCCCCAACTTCCAAAACACAGACGCTGCCTTCTCGGGGCCCCAGAGGTGGGGGCGCTGGAGAG
  CAGCCACCACCCCCCAGTGCCCGTTCCACGCCCTTGCCTGGACCACCAGGCTCCCCGCGCTCCTCCGGCG
  GAACCCCCTTGCACTCACCTCTGCACACGCCTCGAGCCAGCCCTACGGGGACCCCAGGAACGACGCCACC
  TCCCAGCCCAGGCGGTGGCGTCGGGGGAGCCGCCTGGAGAAGTCGGCTCAACTCCATCCGCAACAGCTTC
  CTGGGCTCCCCTCGCTTCCACCGGCGCAAGATGCAGGTCCCTACCGCCGAGGAGATGTCCAGTTTGACAC
  CAGAATCCTCTCCTGAGCTGGCAAAACGCTCCTGGTTCGGGAATTTCATCTCCTTGGACAAAGAAGAACA
  AATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCGGACATTGTCCATGCCTTTCTGTCG
  ATCCCCAGCCTGAGTCACAGTGTACTGTCACAGACCAGCTTCAGGGCCGAATACAAGGCCAGCGGCGGCC
  CCTCCGTCTTCCAGAAGCCTGTCCGCTTTCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAACCCTCACC
  CCGAAGAGATGGCAGCAGTGGAGGTGGCATCTACTCAGTCACCTTTACTCTCATCTCCGGTCCCAGCCGT
  CGGTTCAAACGTGTGGTAGAGACCATCCAGGCACAGTTGCTGAGTACTCACGACCAGCCCTCCGTGCAGG
  CCTTGGCAGATGAGAAGAACGGAGCCCAGACCCGGCCTGCTGGGACCCCACCCCGAAGCCTGCAGCCCCC
  ACCAGGCCGCTCTGACCCTGATCTGAGTAGCTCTCCCCGCCGAGGACCCCCTAAGGACAAGAAGCTCCTG
  GCCACCAATGGAACCCCTCTACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210598 representing NM_001003920
  Red=Cloning site Green=Tags(s)
  
  MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKL
  SESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQI
  VSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGR
  RADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQ
  KHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMI
  YYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRA
  LEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGE
  QPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSF
  LGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLS
  IPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSR
  RFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLL
  ATNGTPLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003920
• ORF Size: 2334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003920.1, NM_001003920.2, NM_001003920.3, NP_001003920.2
• RefSeq Size: 3014 bp
• RefSeq ORF: 2337 bp
• Locus ID: 381979
• Cytogenetics: 7 A1
• MW: 85.6 kDa