Arnt (NM_001037737) Mouse Tagged ORF Clone

CAT#: MR210673

Arnt (Myc-DDK-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arnt
• Synonyms: bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b; mKIAA4051; W08714
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210673 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGACTACAGCTAACCCAGAAATGACATCAGATGTACCATCGCTGGGTCCCACCATTGCTTCTG
  GAAACCCTGGACCTGGGATTCAAGGTGGAGGAGCTGTTGTACAGAGGGCTATTAAGCGACGGTCAGGGCT
  GGATTTTGATGATGAAGTAGAAGTGAACACTAAATTTTTGAGATGCGATGATGACCAGATGTGTAATGAC
  AAAGAGCGGTTTGCCAGGTCGGATGATGAGCAGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATC
  ATAGTGAAATAGAACGGCGGCGACGGAACAAGATGACAGCTTACATCACAGAACTGTCAGACATGGTACC
  TACATGTAGTGCCCTGGCTCGAAAACCAGACAAGCTAACCATCTTACGCATGGCCGTTTCTCACATGAAG
  TCCTTGAGGGGAACTGGCAACACATCTACTGATGGCTCCTACAAGCCATCTTTCCTCACTGATCAGGAAC
  TGAAACATTTGATCTTGGAGGCAGCAGATGGCTTTCTGTTTATTGTCTCCTGTGAGACTGGACGGGTGGT
  GTATGTCTCTGACTCAGTGACTCCCGTTTTGAACCAGCCACAGTCTGAATGGTTCGGGAGCACACTGTAT
  GATCAGGTGCACCCAGATGATGTGGATAAACTTCGAGAGCAGCTCTCTACATCAGAAAATGCCCTAACAG
  GGCGGGTCCTGGATCTGAAGACTGGAACAGTGAAAAAGGAAGGCCAGCAGTCTTCCATGAGGATGTGCAT
  GGGCTCACGAAGGTCGTTCATCTGCCGCATGAGGTGTGGTACTAGCTCCGTGGACCCTGTTTCCATGAAT
  AGACTGAGCTTTTTGAGGAACAGATGCAGGAATGGGCTTGGCTCTGTGAAGGAAGGAGAACCTCACTTTG
  TGGTAGTCCACTGCACAGGCTACATCAAGGCCTGGCCACCAGCAGGTGTCTCCCTCCCAGATGATGACCC
  AGAGGCTGGCCAGGGGAGCAAATTCTGCCTAGTGGCCATTGGCAGGCTGCAGGTAACTAGTTCTCCCAAC
  TGTACAGACATGAGTAACATTTGTCAGCCAACAGAGTTCATCTCCCGACACAACATTGAAGGGATATTCA
  CTTTTGTAGACCATCGTTGTGTGGCTACTGTTGGCTACCAGCCACAGGAGCTCTTAGGGAAGAATATTGT
  AGAATTTTGTCATCCTGAAGACCAACAACTTCTAAGAGACAGCTTTCAGCAGGTGGTGAAATTAAAAGGT
  CAGGTGCTGTCCGTCATGTTCCGATTCCGATCTAAGACCCGAGAATGGCTGTGGATGAGAACGAGCTCCT
  TTACCTTCCAAAACCCTTATTCAGATGAAATTGAGTATATTATCTGCACCAACACCAATGTGAAGAACTC
  TAGCCAGGAACCACGGCCTACACTGTCCAACACCATCCCAAGGTCACAGCTAGGTCCGACAGCCAATTTA
  TCCCTAGAGATGGGTACAGGGCAGCTGCCATCCAGGCAGCAGCAGCAGCAGCACACAGAACTGGATATGG
  TACCAGGAAGAGATGGGCTGGCCAGCTATAATCATTCCCAGGTTTCTGTCCAGCCTGTGGCAAGTGCAGG
  ATCAGAACACAGCAAGCCCCTTGAGAAGTCAGAAGGTCTCTTTGCACAGGACAGAGATCCAAGGTTTCCA
  GAAATCTATCCCAGCATCACTGCAGATCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAAC
  AGCTGTTCTCCCAGGGCAGCTCATTCCCTCCTAACCCCCGGCCGGCAGAGAATTTCAGGAATAGTGGTCT
  TACCCCTCCTGTAACCATTGTCCAGCCATCATCTTCTGCAGGGCAGATACTGGCCCAGATTTCACGTCAC
  TCCAACCCTGCCCAGGGATCAGCGCCGACCTGGACCTCTAGCTCCCGCCCAGGCTTTGCCGCCCAGCAGG
  TGCCCACCCAGGCTACAGCCAAGACTCGTTCTTCCCAATTTGGTGTGAACAACTTTCAGACTTCTTCCTC
  CTTCAGTGCTATGTCTCTTCCGGGTGCTCCCACTGCCTCATCTGGTACTGCTGCCTACCCTGCTCTCCCC
  AACCGTGGCTCCAACTTTCCTCCTGAGACTGGACAGACCACAGGACAGTTCCAGGCCCGGACAGCAGAGG
  GCGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGCCCCATCATCGGTCTAGTTCCAGTGAGCAGCATGT
  TCAGCAGACACAAGCACAAGCACCTAGCCAGCCTGAGGTCTTTCAAGAAATGCTGTCCATGCTGGGAGAC
  CAAAGCAACACCTACAACAATGAAGAATTTCCTGATCTAACTATGTTTCCCCCCTTTTCCGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210673 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATTANPEMTSDVPSLGPTIASGNPGPGIQGGGAVVQRAIKRRSGLDFDDEVEVNTKFLRCDDDQMCND
  KERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMK
  SLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLY
  DQVHPDDVDKLREQLSTSENALTGRVLDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGTSSVDPVSMN
  RLSFLRNRCRNGLGSVKEGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPN
  CTDMSNICQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKG
  QVLSVMFRFRSKTREWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIPRSQLGPTANL
  SLEMGTGQLPSRQQQQQHTELDMVPGRDGLASYNHSQVSVQPVASAGSEHSKPLEKSEGLFAQDRDPRFP
  EIYPSITADQSKGISSSTVPATQQLFSQGSSFPPNPRPAENFRNSGLTPPVTIVQPSSSAGQILAQISRH
  SNPAQGSAPTWTSSSRPGFAAQQVPTQATAKTRSSQFGVNNFQTSSSFSAMSLPGAPTASSGTAAYPALP
  NRGSNFPPETGQTTGQFQARTAEGVGVWPQWQGQQPHHRSSSSEQHVQQTQAQAPSQPEVFQEMLSMLGD
  QSNTYNNEEFPDLTMFPPFSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037737
• ORF Size: 2376 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037737.1, NM_001037737.2, NP_001032826.1
• RefSeq Size: 4370 bp
• RefSeq ORF: 2376 bp
• Locus ID: 11863
• Cytogenetics: 3 40.74 cM
• MW: 87 kDa