Atxn1 (NM_009124) Mouse Tagged ORF Clone

CAT#: MR210675

Atxn1 (Myc-DDK-tagged) - Mouse ataxin 1 (Atxn1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atxn1
• Synonyms: 2900016G23Rik; Atx1; C85907; Gm10786; Sca1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210675 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAAGAAACGTGAGATCCCCGCCACCAGCC
  GGCCCTCGGAGGAGAAGGCCACTGCTCTGCCCAGCGACAACCACTGCGTGGAGGGTGTGGCCTGGCTCCC
  CAGCACCCCTGGCATCCGCGGCCATGGGGGTGGGCGGCACGGGTCAGCAGGGACTTCCGGGGAGCATGGT
  TTACAAGGAATGGGTTTACATAAAGCACTGTCCGCAGGGCTGGATTACTCCCCACCCAGTGCCCCCAGGT
  CAGTCCCCACAGCCAACACGCTGCCCACCGTGTACCCTCCTCCTCAGTCAGGGACCCCGGTGTCTCCTGT
  GCAGTACGCCCACCTTTCGCATACCTTCCAGTTCATTGGGTCCTCCCAATACAGTGGGCCTTACGCGGGC
  TTTATCCCTTCCCAGCTGATCTCCCCATCAGGCAACCCGGTCACCAGTGCAGTAGCCTCAGCTGCAGGGG
  CCACCACTCCATCACAGCGCTCCCAGCTGGAGGCTTATTCCACCCTGCTGGCCAACATGGGCAGTCTGAG
  CCAGGCACCAGGACATAAGGTTGAGCCCCCTCCGCAGCAGCACCTCAGCAGGGCTGCAGGATTAGTCAAC
  CCGGGGTCCCCTCCTCCACCCACCCAGCAGAACCAGTACATCCATATTTCCAGCTCTCCACAGAGCTCCG
  GGCGGGCGACATCTCCCCCACCCATCCCGGTCCACCTCCATCCCCATCAGACGATGATCCCGCACACACT
  CACCCTGGGGCCTTCATCCCAGGTGGTTGTGCAATATAGTGATGCCGGAGGCCACTTTGTTCCTCGAGAG
  TCCACCAAAAAAGCCGAGAGCAGCAGGTTGCAGCAGGCTATGCAAGCCAAGGAAGTCCTGAATGGGGAGA
  TGGAGAAAAGCCGGAGGTATGGGGCATCATCTTCTGTGGAGCTGAGCCTAGGCAAGGCAAGCAGTAAGTC
  AGTGCCTCATCCCTATGAGTCCAGGCATGTGGTGGTCCACCCAAGCCCAGCAGACTACAGCAGTCGTGAT
  ACCTCCGGGGTCCGTGGATCTGTGATGGTTCTGCCTAATAGCAGCACACCCTCAGCCGACCTGGAGGCCC
  AGCAGACCACGCATCGAGAGGCCTCCCCATCCACCCTCAATGACAAGAGCGGCCTGCACCTAGGGAAGCC
  GGGCCACAGGTCTTATGCGCTGTCCCCCCACACGGTCATTCAGACCACACACAGTGCATCAGAGCCTCTC
  CCGGTGGGCCTACCAGCCACGGCCTTCTACGCTGGCACTCAACCTCCTGTCATCGGCTACCTGAGCGGCC
  AGCAGCAAGCAATCACCTATGCTGGTGGTCTGCCGCAGCACCTGGTGATCCCAGGTAACCAGCCCCTGCT
  CATCCCGGTGGGCAGCCCTGACATGGACACGCCTGGGGCAGCCTCGGCCATCGTGACGTCATCACCCCAG
  TTTGCTGCAGTACCTCACACGTTTGTCACCACCGCCCTGCCCAAGAGCGAGAACTTCAACCCAGAGGCTC
  TGGTCACCCAGGCTGCCTACCCAGCCATGGTGCAGGCCCAGATCCACCTGCCGGTGGTGCAGTCCGTGGC
  GTCCCCCACCACGGCGTCTCCCACGCTGCCGCCATATTTCATGAAAGGCTCCATCATCCAGCTGGCCAAC
  GGGGAGCTGAAGAAGGTGGAGGACCTGAAGACGGAGGATTTCATCCAGAGTGCAGAGATTAGCAATGACC
  TCAAGATCGACTCCAGTACTGTGGAGAGAATCGAGGAGAGCCACAGCCCCGGGGTGGCCGTGATACAGTT
  TGCTGTTGGTGAACACCGAGCCCAGGTCAGTGTCGAAGTCTTGGTAGAGTATCCTTTTTTTGTATTTGGA
  CAGGGCTGGTCATCCTGCTGTCCTGAGCGGACCAGCCAGCTCTTTGATCTGCCGTGTTCCAAACTCTCTG
  TTGGGGACGTCTGCATCTCGCTCACCCTCAAGAACCTGAAGAATGGCTCTGTTAAAAAGGGCCAGCCTGT
  GGACCCTGCCAGCGTCCTGCTGAAGCAGGCAAAGACCGACAGCCTGGCTGGCAGCAGACACAGATACGCG
  GAGCAGGAAAACGGAATCAACCAGGGAAGCGCCCAGGTGCTCTCTGAGAATGGCGAACTGAAGTTTCCAG
  AAAAAATAGGATTGCCTGCAGCACCCTTCCTCAGCAAAATAGAACCGAGCAAACCCACAGCCACGAGGAA
  GAGGAGGTGGTCGGCGCCGGAGACCCGTAAACTGGAGAAGTCGGAGGACGAGCCACCTTTGACTCTTCCC
  AAGCCTTCGCTCATTCCTCAGGAGGTTAAGATCTGCATCGAAGGCCGATCTAACGTGGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210675 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKSNQERSNECLPPKKREIPATSRPSEEKATALPSDNHCVEGVAWLPSTPGIRGHGGGRHGSAGTSGEHG
  LQGMGLHKALSAGLDYSPPSAPRSVPTANTLPTVYPPPQSGTPVSPVQYAHLSHTFQFIGSSQYSGPYAG
  FIPSQLISPSGNPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPPPQQHLSRAAGLVN
  PGSPPPPTQQNQYIHISSSPQSSGRATSPPPIPVHLHPHQTMIPHTLTLGPSSQVVVQYSDAGGHFVPRE
  STKKAESSRLQQAMQAKEVLNGEMEKSRRYGASSSVELSLGKASSKSVPHPYESRHVVVHPSPADYSSRD
  TSGVRGSVMVLPNSSTPSADLEAQQTTHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPL
  PVGLPATAFYAGTQPPVIGYLSGQQQAITYAGGLPQHLVIPGNQPLLIPVGSPDMDTPGAASAIVTSSPQ
  FAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPTTASPTLPPYFMKGSIIQLAN
  GELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEESHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFG
  QGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKQAKTDSLAGSRHRYA
  EQENGINQGSAQVLSENGELKFPEKIGLPAAPFLSKIEPSKPTATRKRRWSAPETRKLEKSEDEPPLTLP
  KPSLIPQEVKICIEGRSNVGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009124
• ORF Size: 2376 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009124.2, NM_009124.3, NM_009124.4, NM_009124.5, NM_009124.6, NP_033150.2
• RefSeq Size: 10599 bp
• RefSeq ORF: 2376 bp
• Locus ID: 20238
• MW: 83.8 kDa