Ddx42 (NM_028074) Mouse Tagged ORF Clone

CAT#: MR210774

Ddx42 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (Ddx42)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddx42
• Synonyms: 1810047H21Rik; AW319508; AW556242; B430002H05Rik; RHELP; RNAHP; SF3b125
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210774 representing NM_028074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTGGAATAAAGGCGGCCCTGGAACCAAGCGGGGATTTGGTTTTGGAGGTTTTGCTATCAGTGCTG
  GGAAGAAGGAGGAAGCCAAACTGCCACAGCAGTCCCATAGTGCCTTTGGGGCAGCCAGCTCTTCTTCTGG
  ATTTGGAAAGTCTGCTCCACCACAGCTTCCTTCTTTCTACAAAATTGGATCTAAACGGGCCAACTTTGAT
  GAAGAAAATGCGTATTTTGAAGATGAAGAAGAAGACTCCAGCAATGTAGATTTACCTTACATTCCTGCTG
  AAAACTCACCTACCCGCCAGCAGTTCCATTCCAAGCCCGCAGACTCTGACAGTGATGATGACCCCTTAGA
  GGCATTCATGGCTGAAGTGGAGGATCAGGCTGCTAGAGACATGAAGAGACTTGAAGAAAAGGACAAGGAA
  AGAAAAAACGTCAAGGGTATTCGAGATGACATTGAAGAGGAAGATGACCAAGAAGCTTATTTTCGATACA
  TGGCAGAGAACCCAACTGCTGGGGTGGTTCAGGAGGAAGAAGAGGACAATTTGGAGTATGACAGTGATGG
  AAATCCAATTGCACCTTCCAAAAAAATTATCGATCCCCTTCCTCCCATTGATCATTCGGAGATTGACTAT
  CCACCATTTGAAAAAAATTTTTACAATGAACATGAGGAGATAACCAACCTCACTCCACAGCAGTTGATAG
  ACCTGCGACATAAGCTCAACCTTCGGGTCTCTGGTGCTGCACCTCCCAGACCAGGAAGTAGCTTTGCTCA
  TTTTGGGTTTGATGAACAACTTATGCACCAGATTAGGAAATCTGAGTACACACAGCCGACCCCAATACAG
  TGTCAGGGTGTACCTGTAGCACTAAGTGGGAGAGACATGATTGGTATTGCTAAAACAGGCAGTGGGAAAA
  CTGCAGCCTTTATTTGGCCCATGTTGATTCATATAATGGATCAGAAGGAATTGGAACCAGGTGATGGACC
  AATTGCAGTTATTGTGTGTCCTACTAGGGAGCTTTGTCAACAGATCCACGCAGAGTGTAAGCGGTTTGGG
  AAAGCGTATAATCTTCGATCAGTGGCCGTGTATGGAGGAGGGAGCATGTGGGAGCAGGCCAAGGCCCTTC
  AGGAAGGGGCAGAGATTGTTGTATGTACCCCAGGTCGACTGATTGATCATGTGAAGAAAAAAGCTACTAA
  TCTTCAAAGAGTCTCTTACCTTGTGTTTGATGAAGCCGATCGAATGTTTGACATGGGATTTGAGTATCAG
  GTTCGGTCCATAGCAAGTCATGTTCGTCCTGACAGACAAACTCTCTTATTCAGTGCAACTTTTCGAAAGA
  AGATTGAAAAGTTGGCCAGAGACATCTTGATTGACCCTATTCGAGTGGTGCAGGGAGACATTGGAGAGGC
  GAATGAAGATGTGACACAGATTGTGGAGATTCTCCATTCCGGACCCAGTAAATGGAACTGGCTGACTCGG
  CGTCTGGTGGAGTTTACTTCCTCGGGGAGTGTGCTCTTGTTTGTTACTAAGAAAGCCAATGCTGAAGAGC
  TAGCCAGTAACCTGAAGCAGGAGGGTCATAATCTTGGTCTGCTCCATGGGGACATGGATCAGAGCGAAAG
  AAACAAGGTTATTTCAGATTTTAAGAAAAAGGACATCCCCGTCCTGGTGGCCACTGATGTTGCAGCCCGT
  GGTCTGGATATTCCTTCCATTAAGACAGTCATTAACTATGATGTAGCACGGGACATTGATACGCACACTC
  ACAGGATTGGCCGGACAGGAAGAGCTGGTGAGAAGGGTGTAGCCTATACCTTATTGACTCCAAAGGACAG
  CAATTTTGCTGGTGACCTTGTACGGAACCTGGAAGGAGCCAACCAACATGTTTCTAAGGAGCTCTTAGAT
  CTTGCAATGCAGAATGCCTGGTTTCGGAAATCTCGCTTCAAAGGAGGGAAAGGCAAAAAGCTGAACATTG
  GTGGAGGAGGCTTGGGCTATAGAGAGCGGCCTGGGCTGGGCTCTGAGAATTCGGACCGAGGAAATAATAA
  CAATGTAATGAGCAATTATGAAGCCTACAAGCCCTCCACAGGAGCCATGGGAGATAGGCTCACAGCAATG
  AAAGCAGCTTTTCAGTCCCAGTACAAGAGTCACTTTGTTGCAGCCAGTTTAAGTAATCAGAAGGCCGGAA
  CCTCTTCTGCAGGGGCAAGTGGATGGACTAGTGCAGGAAGCTTAAATTCTGTTCCAACTAATTCAGCACA
  GCAGGGCCATAATAGTCCTGACAACCCCATGACCAGTTCTACCAAGAACATCCCAGGCTTCAACAACTCT
  GGGAATATCAGTAGTGCCCCAGTGACCTACCCTTCTATTGGAGCCCAAGGAGTCAACAACACAGCTTCAG
  GGAATAACAGCCGTGAAGGGATTGGGGGTGGCAATGGGAAGAGAGAGAGATATACTGAGAACCGGGGTGG
  TAGCCGTCATAGCCATGGAGATGGTGGCAATCGGCATGGAGATGGTGGGCGCCATGGAGATGGATACCGT
  TATCCAGAAAGTGGTAGCCGTCATACTGATGGCCACCGACATGGAGAGACCAGGCATGGAGGAAGTGCCG
  GCAGACACGGAGAGAGCCGGGGTGCAAATGATGGCAGGAATGGAGAAAGCAGGAAAGAAGGTTTTAATCG
  TGAAAACAAGATGGACCCCAAGGTGGATAGCAGCAGGATGGACAAAGTGGACAGCAAGACAGATAAGACA
  CCTGATGGCTTTGCTGTTCCAGAGCCACCCAAAAGAAAGAAAAGCCGATGGGATAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210774 representing NM_028074
  Red=Cloning site Green=Tags(s)
  
  MNWNKGGPGTKRGFGFGGFAISAGKKEEAKLPQQSHSAFGAASSSSGFGKSAPPQLPSFYKIGSKRANFD
  EENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPADSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKE
  RKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPSKKIIDPLPPIDHSEIDY
  PPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQ
  CQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFG
  KAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQ
  VRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTR
  RLVEFTSSGSVLLFVTKKANAEELASNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAAR
  GLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLD
  LAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENSDRGNNNNVMSNYEAYKPSTGAMGDRLTAM
  KAAFQSQYKSHFVAASLSNQKAGTSSAGASGWTSAGSLNSVPTNSAQQGHNSPDNPMTSSTKNIPGFNNS
  GNISSAPVTYPSIGAQGVNNTASGNNSREGIGGGNGKRERYTENRGGSRHSHGDGGNRHGDGGRHGDGYR
  YPESGSRHTDGHRHGETRHGGSAGRHGESRGANDGRNGESRKEGFNRENKMDPKVDSSRMDKVDSKTDKT
  PDGFAVPEPPKRKKSRWDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028074
• ORF Size: 2787 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028074.4, NP_082350.3
• RefSeq Size: 4028
• RefSeq ORF: 2790
• Locus ID: 72047
• MW: 102.4 kDa