Tpcn1 (NM_145853) Mouse Tagged ORF Clone

CAT#: MR210795

Tpcn1 (Myc-DDK-tagged) - Mouse two pore channel 1 (Tpcn1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tpcn1
• Synonyms: 5730403B01Rik; mKIAA1169; Tpc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210795 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTAAGTTTAGATGACGATGTGCCGCTCATCCTGACCTTGGACGAGGCTGAGAGTGCTCCGCTGC
  CTCCTTCGAACAGCCTGGGCCAAGAGCAGCTGCCCAGCAAAAATGGGGGCAGCCACAGCATCCACAACTC
  CCAGGTCCCCAGTCTGGTCTCCGGAGCGGACAGCCCCCCCTCCAGTCCCACCGGACACAACTGGGAGATG
  AATTATCAAGAGGCGGCAATCTACCTCCAGGAAGGTCAGAACAACGACAAGTTCTTCACCCACCCCAAGG
  ATGCCAGAGCGCTGGCGGCCTACCTCTTCGTCCACAACCACTTCTTCTACATGATGGAGCTGCTCACGGC
  CCTGCTCCTGCTGCTGCTGTCGCTGTGCGAGTCCCCCGCTGTCCCCGTGCTCAAGCTGCACACTTACGTC
  CACGCCACGCTGGAACTCTTTGCCCTCATGGTGGTGGTATTTGAACTCTGCATGAAATTGCGGTGGCTGG
  GCTTCCACACGTTCGTCCGGCACAAACGTACCATGGTCAAGACGTCCGTCCTCGTGGTGCAGTTCATCGA
  GGCCATTGTGGTGCTGGTTCGGCAGACGTCCCACGTGCGGGTGACCCGGGCACTACGCTGCATTTTCCTG
  GTGGACTGTCGCTACTGTGGCGGTGTACGGCGCAACCTGCGGCAGATCTTCCAGTCTCTCCCACCCTTCA
  TGGACATCCTCCTGTTGCTGCTCTTCTTTATGATCATCTTTGCCATCCTCGGTTTCTACTTATTCTCCAC
  AAATCCTTCCGACCCCTACTTCAGCACCCTGGAGAACAGCATCGTCAACCTGTTCGTTCTCCTGACCACA
  GCCAACTTTCCAGATGTCATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTCTTCTTCATTGTAT
  ACCTCTCCATTGAGCTGTACTTCATCATGAACCTGCTCCTGGCCGTGGTGTTCGACACCTTCAACGACAT
  TGAAAAGCACAAGTTCAAGTCTTTGCTGCTGCACAAACGGACCGCCATCCAGCATGCCTACGGCCTGCTT
  GCCAGCCAACGGAGGCCGGCTGGCATCTCCTACAGGCAGTTCGAAGGCTTAATGCGCTTCTACAAGCCCC
  GGATGAGTGCAAGGGAACGCTTCCTGACTTTCAAGGCCTTGAACCAGAGCAACACGCCTCTGCTCAGCCT
  GAAGGACTTCTATGATATTTACGAAGTCGCTGCTCTGCAGTGGAAGGCAAAGAGAAACAGACAGCATTGG
  TTTGATGAGCTCCCCCGGACAGCCTTCCTCATCTTCAAAGGGATTAACATCCTTGTGAATTCCAAGGCCT
  TCCAGTATTTCATGTACTTGGTGGTGGCTGTCAACGGTGTCTGGATCCTGGTGGAGACATTCATGTTGAA
  AGGTGGGAATTTCACCTCAAAGCATGTGCCCTGGAGTTACCTCGTGTTTCTTACCATCTATGGAGTTGAA
  CTGTTCATGAAGGTGGCTGGCCTGGGCCCTGTGGAGTACCTGTCCTCTGGATGGAACCTGTTCGATTTCT
  CGGTCACGGCATTCGCCTTCCTGGGACTGCTCGCACTGACGCTCAACATGGAACCCTTCTATTTCATTGT
  GGTCCTGCGTCCCCTTCAGCTGCTGAGGTTATTTAAACTGAAGAAACGCTACCGCAACGTGTTGGACACC
  ATGTTTGAGCTGCTGCCGCGGATGGCCAGCCTTGGCCTCACGCTGCTCACCTTCTACTATTCCTTCGCCA
  TCGTGGGCATGGAGTTCTTCAACGGGCGGCTGACCCCCAACTGCTGCAACACCAGCACAGTGGCCGACGC
  CTACCGGTTCATCAACCACACTGTGGGCAATAAGACCAAGGTAGAGGAAGGCTACTACTATCTCAACAAC
  TTTGACAACATCCTCAACAGCTTCGTGACCTTGTTTGAGCTCACCGTTGTCAACAATTGGTACATCATCA
  TGGAAGGCGTCACCTCGCAGACGTCCCACTGGAGCCGCCTGTACTTCATGACCTTTTACATAGTGACCAT
  GGTGGTGATGACCATTATCGTGGCCTTCATCCTGGAGGCCTTCGTCTTCCGCATGAACTACAGCCGCAAG
  AGCCAGGACTCGGAAGTGGACAGTGGCATCGTCATCGAGAAGGAAATGTCCAAGGAGGAGCTTATGGCCG
  TCCTGGAGCTTTATCGTGAGGAGCGAGGCACCTCCTCTGACGTGACCCGGCTGCTGGACACCCTCTCTCA
  GATGGAGAAATACCAGCAAAATTCCATGGTGTTTCTGGGACGGCGATCGAGAACCAAAAGTGACCTGAGT
  CTGAAGATGTACCAGGAGGAGATCCAGGAGTGGTACGAGGAGCATGCCCGGGAACAGGAGCAGCAGAAGC
  TCAGGGGCAGCGTGCCCGGCCCTGCAGCCCAGCAGCCCCCTGGCAGTCGCCAGCGCTCCCAGACTGTCAC
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210795 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVSLDDDVPLILTLDEAESAPLPPSNSLGQEQLPSKNGGSHSIHNSQVPSLVSGADSPPSSPTGHNWEM
  NYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYV
  HATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFL
  VDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTT
  ANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLL
  ASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHW
  FDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSKHVPWSYLVFLTIYGVE
  LFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDT
  MFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNN
  FDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRK
  SQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLS
  LKMYQEEIQEWYEEHAREQEQQKLRGSVPGPAAQQPPGSRQRSQTVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145853
• ORF Size: 2454 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145853.1, NM_145853.2, NP_665852.1
• RefSeq Size: 4666 bp
• RefSeq ORF: 2454 bp
• Locus ID: 252972
• MW: 94.5 kDa