Pign (BC021148) Mouse Tagged ORF Clone

CAT#: MR210838

Pign (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pign
• Synonyms: Gm20308|PIG-N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210838 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTTTTTTGCTTTGGGATTGCTTATCCATTTTGTGTTCTTCGCTTCCATCTTTGACATTTATT
  TTACATCTCCTTTGGTTCATGGAATGACTCCCCAGTTTACACCTTTGCCTCCTCCAGCAAAAAGATTAGT
  GCTATTTGTTGCTGATGGCCTGAGAGCAGATACTCTTTATGAATTAGATGAAGATGGAAACTCCAGAGCA
  CCTTTCATTAGGAATGTTATAATTCATGAAGGCAGCTGGGGAGTATCTCACACACGTGTGCCAACAGAAT
  CTCGGCCTGGCCATGTAGCCTTGATAGCTGGATTTTATGAGGATGTCAGTGCAGTTGCCAAAGGATGGAA
  AGAAAATCCTGTAGAATTTGACTCTCTTTTTAATGAAAGCAAGTACACGTGGAGCTGGGGGAGTCCAGAT
  ATCTTGCCCATGTTTGCCAAAGGTGCTAGTGGTGACCATGTCTATACATATAGTTACGATGCTCAAAGAG
  AAGATTTTGGTGCCCACGATGCCACAAAACTGGATACGTGGGTTTTTGATAAAGTTAAGGACTTCTTTGA
  TGCTGCAAGAAACAACCAATCTTTGTTCACAAAAGTAAATGAAGAAAAAGTAGTTTTTTTCTTACATTTA
  TTGGGAATAGATACAAATGGACATGCTCATCGACCATCGTCAAGGGAATATAAGGACAATATTAAAAAAG
  TTGATGATGGAGTGAAGGAAATCGTATCAATTTTCAAGCATTTTTATGGAGATGATGGGAAAACAGCATT
  TATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCGTCAGAGACTTTA
  ACTCCGTTCGTTACTTGGGGAGCTGGAATCAAATTTCCTCAAAATGTATCAGCTCAGCAATATGATGATG
  AGTTTTTAAAAGAATGGAGATTGGAAAACTGGAAAAGGAGAGATGTCAATCAGGCTGATATTGCACCTCT
  GATGGCATCCCTTATTGGAGTCCCCTTTCCTCTTAATTCGGTGGGAATACTTCCTGTGGGTTATCTTAAC
  AACACTGGTCTCTTCAAAGCAGAGAGCATGTTTACCAATGCAGTACAGATTCTTGAACAGTTCAAGGTGA
  AAATGACTCAGAAAAAAGAAGCTACTTTACCGTTTTTGTTTACACCATTTAAGTTGCTTTCTGATTCTCA
  ACAACTGGACATTTTAAGAAAAGCAAGATCTTACATAAAACAAGAAAAATTTGATGAAGTAGTGTCCCTC
  TGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGG
  GCATCAATGTTGCAGTTGGTTTTGTGGGATGGATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTC
  TAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCT
  ACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGC
  TGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATT
  GACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTC
  AGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGC
  TGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACT
  GATGCCTGTTGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTG
  TCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTC
  TGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAA
  AGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTC
  CTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCC
  TAAGCACTGGGTATGAAGCTCTCTTTCCTTTAGTGCTGTCCTGTTTGATGTTTGTCTGGATCCAAGTGGA
  ACAAGAGACCCTTCAACAGCCTGGTGTTTCTTGTAAACAGAAGCTCACCAGCATCCAGTTCACCTGTGAC
  ACTGATATAGCTCAGTTCCGACAGCTCTGTCCGGATGACATCCGTAGGGCCTTTTTCCTCATATTGTTGA
  TCTTTCCTGTATTCTTAAGACTATTCGACTACAAAAGAATAAGGATTCTGTTAGCCCAAGAAAATTATCA
  TAGGTCATTAAAGGCTGCCTGGGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210838 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLFFALGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRA
  PFIRNVIIHEGSWGVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPD
  ILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHL
  LGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSETL
  TPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPLNSVGILPVGYLN
  NTGLFKAESMFTNAVQILEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSL
  CEELIDLALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYSFIA
  TGVLVACFLMIQACPWTYYVYCLLPVPIWYAVLREHEVIQDLVESLLTFPRSHFVAYLLVFTLGIEVLVL
  SFFYRYMLTAGLIVFAGWPFLTQLWTRAKITFLSWAFFSLLLAVFPLMPVVGRKPNLSLVMGAGFLVLLL
  SLAVVTTLGKRNIKLVKGELLVLLLQMLSTVLSMYVVYSTHHSLLKKEGLPLMNQIVSWATLASSLVAPL
  LSSTALSQRLASILLSLMSTYLLLSTGYEALFPLVLSCLMFVWIQVEQETLQQPGVSCKQKLTSIQFTCD
  TDIAQFRQLCPDDIRRAFFLILLIFPVFLRLFDYKRIRILLAQENYHRSLKAAWEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC021148
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC021148, AAH21148
• RefSeq Size: 4038
• RefSeq ORF: 2480
• Locus ID: 27392
• MW: 93.8 kDa