Cars (NM_013742) Mouse Tagged ORF Clone

CAT#: MR210862

Cars (Myc-DDK-tagged) - Mouse cysteinyl-tRNA synthetase (Cars)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cars
• Synonyms: CA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210862 representing NM_013742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTTCCTCCGCGGAGCAGGCTGCCGACTACAGGTCCATTCTGAGCATTAGTGACGAGGCAGCCA
  GGGTGCAGGCCCTGGACCAACACCTCAGCACGCGTAGCTATATCCAGGGGTACTCCCTATCCCAGGCAGA
  TGTGGACGTGTTCAGGCAGCTCTCGGCCCCTCCCGCTGACTCAAGGCTCTTCCACGTGGCTCGGTGGTTC
  AGGCACATAGAAGCGCTCCTGGGTGGCCCCCAAGGCAGAGACGAGCCCTGCAGGCTTCAAGCAAGTAAAG
  GCCGGCGAGTGCAGCCTCAGTGGTCCCCCCCAGCAGGGACTGAGCCCTGCAGGCTCCGCCTCTATAATAG
  CCTCACCCGGAACAAGGATGTATTTATACCTCAAGATGGGAAGAAGGTGACGTGGTACTGTTGCGGGCCG
  ACTGTCTATGATGCGTCTCACATGGGACATGCCAGGTCCTACATCTCCTTCGATATCCTGAGGAGGGTGC
  TGAGGGATTACTTCCAGTATGATGTCTTTTACTGCATGAACATCACAGACATTGATGATAAGATCATCAG
  GCGGGCACGGCAGAACTACCTGTTTGAGCAGTATCGGGAGCAGAAACCCCCAGCCACCCAGCTCCTGAAG
  GATGTCCGTGATGCCATGAAGCCATTTTCAGTCAAGTTAAGTGAGACAACAGATCCCGACAAGAGGCAGA
  TGCTGGAGCGGATCCAGAACTCTGTGAAACTTGCCACAGAACCACTGGAACAGGCTGTGCGATCCAGCCT
  CTCTGGAGAGGAAGTAGACAGCAAAGTACAGGTGTTGCTGGAGGAAGCAAAGGACTTGCTCTCTGACTGG
  CTGGATTCCACAGGTGGCAGTGAGGTGACTGACAACTCGATTTTCTCCAAACTGCCCAAGTTCTGGGAAG
  AAGAGTTCCACAAAGACATGGAAGCCCTGAATGTTCTCCCTCCTGATGTCCTAACCCGTGTCAGTGAATA
  TGTGCCAGAAATTGTGAACTTCGTCCAGAAGATTGTGGACAATGGTTATGGTTATGCTTCAAATGGATCT
  GTTTACTTCGACACAGCCAAGTTTGCTGCTAGTGAAAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCG
  TTGGGGATCAGAAGGCACTTCAGGAAGGGGAAGGTGATCTGAGCATCTCTGCTGACCGCCTTAGTGAGAA
  ACGCTCTCCCAATGACTTTGCCTTGTGGAAGGCCTCCAAGCCAGGCGAGCCATCCTGGCCTTGCCCCTGG
  GGAAAGGGTCGTCCCGGATGGCACATAGAGTGTTCTGCTATGGCAGGCACGCTCCTGGGAGCCTCAATGG
  ACATTCATGGTGGAGGGTTTGACCTCCGCTTCCCCCACCATGACAATGAGCTGGCACAGTCGGAGGCCTA
  CTTTGAAAATGACTGCTGGGTCAGGTACTTCTTGCACACGGGCCACTTGACGATAGCAGGCTGCAAGATG
  TCCAAGTCACTGAAAAACTTTATCACCATTAAAGATGCCTTGAAGAAGCACTCAGCACGGCAGCTGCGAC
  TGGCATTCCTCATGCACTCATGGAAAGACACACTGGACTATTCCAGCAACACTATGGAGTCTGCTCTTCA
  GTATGAAAAATTCATGAACGAGTTTTTCTTAAATGTGAAAGACATCCTCCGAGCCCCTGTAGACATCACT
  GGCCAGTTTGAAAAGTGGGAAGCTGAAGAAGTGGAGCTAAATAAGAACTTCTATGGCAAGAAGACCGCAG
  TTCACGAAGCCTTGTGTGATAACATTGACACCCGGACTGTCATGGAAGAGATGCGGGCTTTGGTCAGTCA
  GTGCAACCTCTACATGGCAGCCAGGAAGGCTGAACGGAGGAGGCCCAACCGGGCTCTGCTGGAGAACATT
  GCCATGTACCTCACCCACATGCTGAAGATCTTTGGGGCCATAGAGGAGGAGAGCCCCCTGGGGTTCCCAG
  TTGGTGGGCCTGGAACCAACCTGAACCTCGAGTCAACAGTCATGCCCTACCTTCAGGTGTTATCAGAATT
  CAGAGAGGGAGTACGAAAGATTGCCCGAGAGAAAAAAGTTCTTGAGGTTCTACAGCTCAGTGATGCCCTC
  CGGGATGACATCCTGCCTGAGCTTGGGGTCCGGTTTGAAGACCATGAAGGGCTGCCAACAGTGGTGAAGT
  TGGTGGACAGAGACACCTTACTGAAAGAGAAGGAAGGAAAGAAAAGGGCTGAAGAAGAGAAGAGGAGGAA
  GAAAGAGGAGGCGGCCAGAAAGAAACAGGAGCAAGAAGCAGCAAAGCTGGCCAAGATGAAGATACCACCC
  AGTGAGATGTTCCTGTCAGAAGTCAACAAGTATTCTAAATTTGATGAAAATGGTCTGCCTACCCACGACA
  CCGAAGGCAAAGAGCTGAGCAAGGGCCAGGCCAAGAAGCTGAAGAAGCTCTTTGAAGCCCAGGAGAAACT
  GTACAAGGAGTATCTGCAAATGTTACAGAATGGCAGCCTCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210862 representing NM_013742
  Red=Cloning site Green=Tags(s)
  
  MAGSSAEQAADYRSILSISDEAARVQALDQHLSTRSYIQGYSLSQADVDVFRQLSAPPADSRLFHVARWF
  RHIEALLGGPQGRDEPCRLQASKGRRVQPQWSPPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGP
  TVYDASHMGHARSYISFDILRRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLK
  DVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDW
  LDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGS
  VYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPW
  GKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKM
  SKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDIT
  GQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENI
  AMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDAL
  RDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPP
  SEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013742
• ORF Size: 2493 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013742.1, NM_013742.2, NM_013742.3, NM_013742.4, NM_013742.5, NP_038770.3
• RefSeq Size: 3236
• RefSeq ORF: 2496
• Locus ID: 27267
• MW: 94.9 kDa