Bahcc1 (BC060615) Mouse Tagged ORF Clone

CAT#: MR210869

  • TrueORF®

Bahcc1 (Myc-DDK-tagged) - Mouse BAH domain and coiled-coil containing 1 (cDNA clone MGC:79150 IMAGE:6417635)


  "BC060615" in other vectors (4)


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Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Bahcc1
Synonyms MGC79150, B930044J06
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210869 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCAGCCAGTCCTGAGGCGGAAGAATGGGGCCTTGTCCATCGCTCTGTCAGCCCGCAACGCCAAGG
CCATCCTGGGAAAGAGCAGGAAGCTGACGAAGGTGAAGAGAGAGGCTGTCAGCAAGCAGGGCCAGGGGCG
GGCGGTGAGCCGGCTGCTGGAGAGCTTTGCTGTGGAGGATGACTTTGAGTTTGACGAGGATGACACCAGC
TTCTCGGATGAGGAAGAAGAAGAGGAAGAAGCCGGTGTACAGCTCAGCGCAGAGCAGAGCGCCGCCCTGG
CACGGTCCTGCACCATCCACAAGGAAGACCTGCAGGACGGACTGCCCGTCCTGATCCCGAAGGAGGACAG
CCTGCTGTATGCGGGCAGTGTCAGGACCCTGCAACCCCCCGACATCTACAGCATCGTCATTGAGGGGGAG
AGAGGCAACCGTCAACGTATCTACTCGCTGGAACAGCTGCTGCAGGAGGCGGTTCTTGATGTCCAGCCAC
AGTCCAGTCGGTACCTTCCACCTGGTACCCGGGTCTGTGCCTACTGGAGTCAGAAGTCCCGGTGTCTATA
TCCAGGCAATGTGGTTCGAGGTGCTTCAAGCGATGAAGAAGACCTGGACTCAGTGTTGGTAGAGTTCGAT
GACGGAGACACAGGCCACATAGCTGTCTCTAACATCAGGCTGCTGCCTCCGGACTTCAAGATCCAGTGTA
CAGAGCCCTCGCCAGCCCTGTTGGTGTCCAGCAGCTGCCGGAGGACCAAAAAAGCAGCCAATGAGGGCCA
CCCGCCCAGCGAAGCCCCCACACCCAGCCTGTCCCCTAAAGTGCCGGATGGCCCTGAAACTTCTAAGACC
CCTGGGAAGAAATCTGGCAGCAAAGACAAAGCTGGCAAAGTCGACCTCCTAACCTCAGGTGCCAAGTCCC
CCACGGGGGCCTCAGACCACTTCCTAGGCCGCAGAGGCAGCCCCCTGCTGAGCTGGTCAGCAGTGGCTCA
GACCAAGCGGAAAGCGGTGGCTGCGGCAGCGGCGGCGGCAGGTGGCAAAGGGCCGGGGGTACTACAGAAC
CTCTTCCAGCTCAACGGAAGCACCAAGAAGCTGCGGGCCCGGGACACCCTGTTTCCCATGCATAGCATGG
CTACCCCCGTGTTTGGTAACAGCTTCCGAGCTGACTCCTTCAGCAGCCTGGCGAGTTCCTACACACCCTT
CCTTGGAGGGGCTGGGGCAGGCCTTCCCGGAGGAGCCCACAAGCTGCTTAGGGCCAAGAAGGCTGAACGG
GCTGAAGCAGAAAAGGCCGGGAGGCGGCGGGCAGGCGGCGAGTTTCTGGTTAAGCTGGACCATGAGGGTG
TGACCTCCCCCAAAAACAAAAACTGCAAGGCCCTGCTCATGAGCGACAAGGACTTCGGACCCAAGCTGGG
GCGACCTCTTAGCAACCCCAGTTACGCACACCCAGCCCTCATTGGCAAGGACAAGAAGGGTCGGGCGCCT
GTGCATCCGCTACCCATGGGGCTGGCTCTGCGCAAGTACCCACTGCCCTGTGATAGTGACTGCCCCAGCT
CCTACTCCGATGAGGACGAGGATGGGCCGGGGCTGGCCACCGGTGTTCCCTCCCGATTCCTCACCCGCCT
ATCCATGTCGTCGTCCTCCTCTGGCTCGTCCACGTCTTCTTCCTCAGGCTCCGTGTCCACGTCCAGCCTC
TGTTCCTCAGACAATGAGGACTCATCTTATAGCTCAGATGACGAGGACCCTGCCCTGCTGCTGCAGACCT
GTCTCACCCGCCCTGTGCCCGCCCTCCTGGCCCCACCCGAAGCCCTGCGCTCTAAGGGTAGCAGCCCCCA
CGCGCACACCCACGCCCAGCGCTGCTTCCTGTCCAGGGCTGGGGTGGCTGGTGCAGGTGCAGGGGCCAGC
CCCAGTGGTAGCAAATCCAAGTTCAAGCGCAAGGAGGCCCTGAGCTTCTCCAAAGCCAAAGAGCTTTCTC
GGAGGCAACGGCTGCCCTCCGTAGAAAACCGGCCAAAGATCTCAGCCTTCCTGCCTGCCCGGCAGCTCTG
GAAGTGGTCAGGAAACCCCACGCAGAGACGAGGCATGAAGGGGAAAGCCAGGAAACTATTCTACAAGGCC
ATTGTCCGAGGCAAGGAGACACTGCGCATCGGGGACTGTGCGGTCTTCCTTTCGGCCGGCCGGCCCAACC
TGCCCTACATAGGCCGCATCGAGAGCTTGTGGGAGTCCTGGGGCAGCAACATGGTGGTGAAGGTCAAATG
GTTCTACCACCCAGAGGAGACCAAGCTGGGGAAGCGGCAGAGTGACGGGAAGAACGCACTGTACCAGTCC
TGCCACGAAGATGAGAATGACGTGCAGACCATCTCCCACAAATGTCAGGTGGTGGGCCGAGAGCAGTATG
AACAGATGATGCGGGGCCGCAAGTACCAGGACCAGCAGGACCTGTACTACTTGGCAGGCACCTATGATCC
TACTACTGGGCGCCTGGTGACAGCTGACGGCGTGCCCGTCCTGTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210869 protein sequence
Red=Cloning site Green=Tags(s)

MLQPVLRRKNGALSIALSARNAKAILGKSRKLTKVKREAVSKQGQGRAVSRLLESFAVEDDFEFDEDDTS
FSDEEEEEEEAGVQLSAEQSAALARSCTIHKEDLQDGLPVLIPKEDSLLYAGSVRTLQPPDIYSIVIEGE
RGNRQRIYSLEQLLQEAVLDVQPQSSRYLPPGTRVCAYWSQKSRCLYPGNVVRGASSDEEDLDSVLVEFD
DGDTGHIAVSNIRLLPPDFKIQCTEPSPALLVSSSCRRTKKAANEGHPPSEAPTPSLSPKVPDGPETSKT
PGKKSGSKDKAGKVDLLTSGAKSPTGASDHFLGRRGSPLLSWSAVAQTKRKAVAAAAAAAGGKGPGVLQN
LFQLNGSTKKLRARDTLFPMHSMATPVFGNSFRADSFSSLASSYTPFLGGAGAGLPGGAHKLLRAKKAER
AEAEKAGRRRAGGEFLVKLDHEGVTSPKNKNCKALLMSDKDFGPKLGRPLSNPSYAHPALIGKDKKGRAP
VHPLPMGLALRKYPLPCDSDCPSSYSDEDEDGPGLATGVPSRFLTRLSMSSSSSGSSTSSSSGSVSTSSL
CSSDNEDSSYSSDDEDPALLLQTCLTRPVPALLAPPEALRSKGSSPHAHTHAQRCFLSRAGVAGAGAGAS
PSGSKSKFKRKEALSFSKAKELSRRQRLPSVENRPKISAFLPARQLWKWSGNPTQRRGMKGKARKLFYKA
IVRGKETLRIGDCAVFLSAGRPNLPYIGRIESLWESWGSNMVVKVKWFYHPEETKLGKRQSDGKNALYQS
CHEDENDVQTISHKCQVVGREQYEQMMRGRKYQDQQDLYYLAGTYDPTTGRLVTADGVPVLC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060615
ORF Size 2496 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC060615, AAH60615
RefSeq Size 5004 bp
RefSeq ORF 2498 bp
Locus ID 268515
MW 89.9 kDa

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