Sidt2 (NM_172257) Mouse Tagged ORF Clone

CAT#: MR210871

Sidt2 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sidt2
• Synonyms: B930096O19; BC023957; CGI-40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210871 representing NM_172257
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCCTGGCGTCTGCCCTTGTGCGTGCTCTTGGTGGCCTCCGTCGAGAGCCACCTGGGGGCCCTGG
  GGCCCAAGAACGTCTCGCAGAAAGACGCGGAGTTTGAGCGCACCTACGCGGACGACGTCAACAGCGAGCT
  GGTCAACATCTACACCTTCAACCACACCGTGACCCGCAACCGGACCGAGGGTGTGCGAGTGTCTGTGAAT
  GTCCTGAACAAGCAGAAAGGGGCGCCTTTGCTGTTTGTGGTCCGCCAGAAGGAGGCTGTTGTGTCCTTCC
  AGGTGCCCCTAATCCTTCGAGGACTATATCAGCGGAAGTACCTCTACCAAAAAGTGGAACGAACTCTGTG
  TCAGCCCCCCACCAAGAATGAGTCTGAGATCCAGTTTTTCTATGTGGACGTGTCTACCCTGTCACCCGTC
  AATACCACTTACCAGCTCCGAGTCAACCGTGTGGACAATTTTGTGCTCAGGACTGGAGAGCTGTTTACTT
  TTAATACCACTGCAGCCCAGCCCCAGTACTTCAAATACGAGTTTCCTGATGGTGTGGACTCGGTAATTGT
  CAAGGTGACCTCCAAGAAGGCCTTCCCCTGCTCAGTCATCTCCATCCAAGATGTCCTGTGCCCTGTCTAT
  GATCTGGACAACAATGTAGCCTTCATTGGCATGTACCAGACGATGACTAAGAAGGCAGCCATCACTGTGC
  AGCGGAAAGACTTCCCCAGCAACAGCTTCTATGTGGTGGTGGTAGTGAAGACTGAGGACCAGGCCTGCGG
  AGGGTCCTTGCCCTTCTACCCTTTTGTGGAAGATGAGCCAGTGGATCAAGGGCACCGTCAGAAAACACTG
  TCAGTGCTGGTCTCTCAGGCTGTCACATCTGAGGCCTATGTTGGTGGGATGCTCTTTTGCCTGGGCATAT
  TCTTGTCCTTCTACCTGCTGACTGTGCTGCTGGCCTGTTGGGAGAACTGGAGGCAAAGGAAGAAGACCTT
  GCTGTTGGCCATAGACCGAGCCTGCCCAGAAAGTGGTCACGCCCGGGTCTTGGCTGATTCATTTCCTGGC
  AGTGCCCCTTACGAGGGTTACAACTATGGCTCCTTTGAAAATGGTTCCGGATCCACTGACGGGTTGGTTG
  AAAGCGCAGGTTCAGGGGACCTCTCCTACAGTTACCAGGACCGCTCCTTTGACGCAGTGGGTCCTCGGCC
  TCGACTGGACTCCATGAGCTCCGTGGAAGAGGATGACTACGACACACTGACTGACATCGACTCAGACAAA
  AACGTCATTCGAACCAAGCAATACCTCTGTGTGGCTGATCTGGCACGAAAGGACAAACGTGTTTTGCGGA
  AAAAGTACCAGATTTACTTCTGGAACATAGCCACCATTGCGGTCTTCTACGCACTTCCTGTGGTGCAGCT
  GGTGATCACCTACCAGACGGTGGTGAATGTCACAGGGAACCAGGACATCTGCTACTACAACTTCCTCTGT
  GCCCACCCGCTGGGCAACCTCAGCGCCTTCAACAACATCCTCAGCAACTTGGGGTACATCCTGCTGGGGC
  TGCTCTTCCTGCTCATCATCCTGCAGCGAGAGATCAATCATAACCGGGCCCTGCTGCGGAATGACCTCTA
  TGCTCTGGAGTGTGGGATCCCCAAACACTTTGGTCTGTTTTACGCCATGGGCACAGCACTGATGATGGAG
  GGGCTACTTAGTGCCTGTTACCACGTCTGCCCCAACTACACCAACTTCCAGTTTGATACCTCCTTCATGT
  ACATGATTGCTGGCCTCTGCATGCTGAAGCTCTACCAGAAGCGGCACCCAGATATCAACGCCAGTGCCTA
  CAGTGCATATGCCTGCTTGGCCATCGTCATCTTCTTCTCCGTTCTGGGCGTGGTGTTTGGCAAAGGGAAC
  ACGGCCTTCTGGATTGTCTTCTCCGTCATTCACATCATCTCCACCCTGCTCCTCAGCACTCAGCTCTATT
  ACATGGGCCGCTGGAAGCTGGACTCCGGGATCTTCCGCCGCATCCTCCATGTGCTCTACACAGACTGCAT
  CCGGCAGTGCAGCGGGCCCCTTTACACGGACCGCATGGTGCTTCTGGTCATGGGCAACATTATCAACTGG
  TCGCTGGCTGCATACGGACTCATCATGCGCCCCAATGACTTTGCTTCCTACTTGCTGGCAATTGGCATCT
  GCAACCTGCTGCTTTATTTCGCCTTCTACATCATCATGAAGCTCCGGAGCGGCGAGAGGATCAAGCTCAT
  CCCTCTGCTCTGCATCGTCTGCACCTCCGTGGTCTGGGGCTTCGCGCTCTTCTTCTTCTTCCAGGGACTG
  AGCACGTGGCAGAAAACCCCCGCAGAGTCCAGGGAGCACAACCGCGACTGCATCCTCCTCGACTTCTTTG
  ATGACCACGATATCTGGCACTTCCTGTCCTCCATTGCCATGTTTGGGTCCTTCCTGGTTTTGCTGACGTT
  GGATGACGACTTGGACACAGTACAGCGGGACAAGATCTATGTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210871 representing NM_172257
  Red=Cloning site Green=Tags(s)
  
  MIAWRLPLCVLLVASVESHLGALGPKNVSQKDAEFERTYADDVNSELVNIYTFNHTVTRNRTEGVRVSVN
  VLNKQKGAPLLFVVRQKEAVVSFQVPLILRGLYQRKYLYQKVERTLCQPPTKNESEIQFFYVDVSTLSPV
  NTTYQLRVNRVDNFVLRTGELFTFNTTAAQPQYFKYEFPDGVDSVIVKVTSKKAFPCSVISIQDVLCPVY
  DLDNNVAFIGMYQTMTKKAAITVQRKDFPSNSFYVVVVVKTEDQACGGSLPFYPFVEDEPVDQGHRQKTL
  SVLVSQAVTSEAYVGGMLFCLGIFLSFYLLTVLLACWENWRQRKKTLLLAIDRACPESGHARVLADSFPG
  SAPYEGYNYGSFENGSGSTDGLVESAGSGDLSYSYQDRSFDAVGPRPRLDSMSSVEEDDYDTLTDIDSDK
  NVIRTKQYLCVADLARKDKRVLRKKYQIYFWNIATIAVFYALPVVQLVITYQTVVNVTGNQDICYYNFLC
  AHPLGNLSAFNNILSNLGYILLGLLFLLIILQREINHNRALLRNDLYALECGIPKHFGLFYAMGTALMME
  GLLSACYHVCPNYTNFQFDTSFMYMIAGLCMLKLYQKRHPDINASAYSAYACLAIVIFFSVLGVVFGKGN
  TAFWIVFSVIHIISTLLLSTQLYYMGRWKLDSGIFRRILHVLYTDCIRQCSGPLYTDRMVLLVMGNIINW
  SLAAYGLIMRPNDFASYLLAIGICNLLLYFAFYIIMKLRSGERIKLIPLLCIVCTSVVWGFALFFFFQGL
  STWQKTPAESREHNRDCILLDFFDDHDIWHFLSSIAMFGSFLVLLTLDDDLDTVQRDKIYVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172257
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172257.1, NM_172257.2, NM_172257.3, NM_172257.4, NP_758461.1
• RefSeq Size: 4221 bp
• RefSeq ORF: 2499 bp
• Locus ID: 214597
• MW: 95 kDa