Lrp12 (BC058345) Mouse Tagged ORF Clone

CAT#: MR210912

Lrp12 (Myc-DDK-tagged) - Mouse low density lipoprotein-related protein 12 (cDNA clone MGC:66539 IMAGE:6822657)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrp12
• Synonyms: AI848829|C820005L12Rik|LRP-12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210912 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGTCGCTGGAGCACAAAAGAGTCTCAGCGGCGGGGGTCGGCGTGGCTCTTGCTTTTCCTCGCTG
  GGGTGTACGCTTGTGGGGAGAGTCCAGAACAAATTCGAGCACCGAGTGGAATAATCACGAGTCCAGGCTG
  GCCTTCAGACTATCCTGCCCAAGTCAACTGCAGCTGGCTCATAAGGGCAAACCCAGGGGAGATCATTACT
  ATAAGTTTTCAGGATTTTGATATCCAGGGGTCCAGAAGATGCACTTTGGACTGGCTGACAATAGAAACAT
  ACAAGAATATTGAAAGTTACCGAGCGTGTGGGTCCACAATTCCGCCACCATATATCTCTTCCCAAGACCA
  CGTTTGGATCCGGTTCCACTCAGATGACAGCGTGTCCAGGAAAGGCTTCAGGCTGGCGTATTTTTCAGGG
  AAATCTGAGCAGCCAGACTGTGCCTGTGATCAGTTCCGCTGTGGAAATGGAAAGTGTATTCCAGAAGCCT
  GGAAGTGCAACAGCATGGACGAGTGTGGAGACAGTTCTGACGAGGAGGTCTGTGCCAGCGATGCTCACCC
  CCCAACCACGACTGCTTTCCAACCCTGTGCCTACAACCAGTTCCAGTGTCTGTCCCGCTTCACCAAAGTT
  TACACGTGTCTCCCTGAGTCCTTAAAGTGTGATGGGAACATTGACTGCCTGGACCTAGGAGACGAGATAG
  ACTGTGACATGCCGACATGCGGACAATGGTTAAAATACTTCTATGGCACCTTCAATTCTCCCAACTACCC
  CGACTTTTATCCTCCTGGAAGTAACTGCACCTGGCTGATAGACACTGGTGATCATCGGAAAGTCATCTTA
  CGGTTCACTGACTTTAAACTTGATGGTACCGGGTATGGTGATTATGTCAAAATATATGACGGACTGGAGG
  AGAATCCTCGCAAGCTCTTGCGGGTGCTAACTGCTTTCGATTCTCACGCTCCTCTGACGGTCGTTTCTTC
  CTCCGGACAGATAAGAGTACATTTCTGTGCTGATAAAGTGAATGCTGCAAGAGGGTTCAACGCTACTTAC
  CAAGTCGATGGCTTCTGTTTGCCATGGGAAATACCCTGTGGGGGCAACTGGGGGTGCTACACAGAGCAGC
  AGCGCTGTGACGGGTACTGGCATTGCCCAAACGGAAGGGATGAAATCAATTGTACCATGTGCCAAAAGGA
  AGAATTTCCATGTTCTCGAAATGGTGTCTGCTACCCTCGTTCTGATCGCTGCAACTACCAAAACCATTGT
  CCGAACGGCTCTGATGAAAAAAACTGCTTCTTTTGCCAGCCAGGGAATTTCCACTGTAAGAACAACCGCT
  GTGTGTTTGAAAGCTGGGTGTGTGATTCCCAGGATGACTGCGGTGACGGCAGTGACGAGGAGAACTGCCC
  AGTCATTGTGCCTACCAGAGTCATAACAGCAGCAGTCATCGGGAGCCTCATCTGTGGCCTGCTGCTCGTC
  ATTGCATTGGGGTGCACTTGCAAGCTTTATTCTCTGAGAATGTTTGAACGGAGATCATTTGAGACCCAGT
  TGTCACGCGTGGAAGCAGAACTTCTCCGAAGAGAGGCTCCTCCCTCATATGGACAGTTGATCGCTCAGGG
  ACTAATCCCACCCGTAGAAGATTTTCCTGTCTGTTCACCTAATCAGGCTTCCGTTTTAGAAAACCTGAGG
  CTAGCTGTCCGATCTCAGCTGGGATTTACTTCAATCAGGCTTCCTATGACAGGCAGATCTAGCAACATTT
  GGAATCGTATTTTTAATTTTGCAAGATCCCGTCATTCAGGGTCATTGGCTTTGGTCTCGGGAGATGGAGA
  TGAGGTTGTCCCTAGCCAGAGCAGCAGCAGAGAAACTGAGAGGAGTCGCCCTCACAGAAGCTTATTCTCC
  GTGGAGTCCGATGACACTGACACAGAAAATGAGAGAAGGGATACGGCGGGAGCATCGGGTGGGGTTGCAG
  CACCGTTGCCCCAGAAGGTCCCTCCCACGACCGCCGTGGAGGCCACAGTGGGATCAGGTGGGAATTCCTC
  TGCTCAGAGCACCAGAGGTGGCCATGCAGATGGAAGGGAAGTGTCAAGTGTGGAAGCCCCGAGTGTGAGC
  CCAGCACGTCACCAGCTCACAAGTGCTTTAAGTCGGATGACTCAGGGCCTGCGCTGGGTCCGCTTTACAC
  TAGGTCGATCGAGCTCCACTACCCAGAACCGGAGTCCTTTGAGACAGCTGGACACCGCAGTGAGTGGACG
  AGAAGACGACGATGACGTCGAAATGCTAATTCCAGTTTCAGACGGAGCTTCGGACATTGATGCCAATGAC
  TGCTCCAGACCTCTTCTTGACCTTGCCTCAGACCAAGTACAAGGGTTTAGACAGCCACACAGTGCAGGAA
  ACCCTGGAGTGAGAACAAGTAACCGGGACGGCCCCTGCGAGCGCTGTGGCATTGTGCACACAGCCCAGAT
  ACCAGACACTTGCTTAGAAGCCACAGTGAAGACGGAAACGAGTGATGACGAGGCTTTGCTGCTGTGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210912 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARRWSTKESQRRGSAWLLLFLAGVYACGESPEQIRAPSGIITSPGWPSDYPAQVNCSWLIRANPGEIIT
  ISFQDFDIQGSRRCTLDWLTIETYKNIESYRACGSTIPPPYISSQDHVWIRFHSDDSVSRKGFRLAYFSG
  KSEQPDCACDQFRCGNGKCIPEAWKCNSMDECGDSSDEEVCASDAHPPTTTAFQPCAYNQFQCLSRFTKV
  YTCLPESLKCDGNIDCLDLGDEIDCDMPTCGQWLKYFYGTFNSPNYPDFYPPGSNCTWLIDTGDHRKVIL
  RFTDFKLDGTGYGDYVKIYDGLEENPRKLLRVLTAFDSHAPLTVVSSSGQIRVHFCADKVNAARGFNATY
  QVDGFCLPWEIPCGGNWGCYTEQQRCDGYWHCPNGRDEINCTMCQKEEFPCSRNGVCYPRSDRCNYQNHC
  PNGSDEKNCFFCQPGNFHCKNNRCVFESWVCDSQDDCGDGSDEENCPVIVPTRVITAAVIGSLICGLLLV
  IALGCTCKLYSLRMFERRSFETQLSRVEAELLRREAPPSYGQLIAQGLIPPVEDFPVCSPNQASVLENLR
  LAVRSQLGFTSIRLPMTGRSSNIWNRIFNFARSRHSGSLALVSGDGDEVVPSQSSSRETERSRPHRSLFS
  VESDDTDTENERRDTAGASGGVAAPLPQKVPPTTAVEATVGSGGNSSAQSTRGGHADGREVSSVEAPSVS
  PARHQLTSALSRMTQGLRWVRFTLGRSSSTTQNRSPLRQLDTAVSGREDDDDVEMLIPVSDGASDIDAND
  CSRPLLDLASDQVQGFRQPHSAGNPGVRTSNRDGPCERCGIVHTAQIPDTCLEATVKTETSDDEALLLC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC058345
• ORF Size: 2517 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058345, AAH58345
• RefSeq Size: 4389 bp
• RefSeq ORF: 2519 bp
• Locus ID: 239393
• MW: 92.7 kDa