Ube4a (NM_145400) Mouse Tagged ORF Clone

CAT#: MR210961

Ube4a (Myc-DDK-tagged) - Mouse ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) (Ube4a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ube4a
• Synonyms: 4732444G18Rik; 9930123J21Rik; UFD2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210961 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGAGCAACTCAACATCAATCACATGATCCAGAGGATCTTCCTCATCACCCTGGACAACAGTGATC
  CAAGCTTGAAAAGCGGGAATGGCATCCCCAGCCGTTGTGTGTACTTGGAAGAAATGGCAGTAGAGCTGGA
  AGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCATCTTTGCTCGATTATTGCTTCAAGATCCA
  GGAAACCACTTGATCAGCATGACTTCCTCCACAACTTTAAATCTCTCTGCTGATCGTGATGCAGGAGAGA
  GGCACATCTTCTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGAGATTACCAAAGTTCCAGAAAA
  CCTACTCCCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAACACTCGAACAGTTCTCCTCACCCCA
  GAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGTTGGAAGCTATTCAGGGAGCCC
  ATTTTGAAGATGTAACTGAATTTTTAGAAGAGGTCATTGAAGCCTTGCTGTTGGATGAGGAGGTTCGAAC
  ATTTCCTGAAGTCATGATTCCAGTGTTTGATATTTTACTCAGCCGAATAAAAGATCTGGAACTCTGCCAG
  ATCCTCCTGTATGCATATCTGGACATTCTTCTCTATTTCACTAGGCAGAAGGATATGGCCAAGGTGTTTT
  TAGAATACATTCAGCCCAAGGATCCCAGCAATGGGCAGATGTATCAGAAGACCTTGCTGGGAGTCATCCT
  GAATATCTCGTGCTTGTTAAAGACTCCGGGTGTGGTAGAGAACCATGGCTTCTTCCTGAATCCATCCCGT
  TCCAGTCCTCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGTTCCATGAAAAGA
  TCTACCAGATGCTGAAGAACTTGCTCCAGCTCTCTCCAGAAACCAAGCACTGTATCTTGTTCTGGCTTGG
  AAACTGTTTGCATGCAAATGCAGGCCGCACCAAGATCTGGGCCAATCAGATGCCCGAAATCTTCTTCCAA
  ATGTATGCCTCGGATGCCTTCTTTCTGAATCTGGGTGCTGCCCTCCTGAAGCTGTGCCAGCCATTCTGCA
  AGCCCAGATCCTCCCGGCTCCTCACCTTTAACCCCACGTACTGTGTCCTCAAGGACTTGAATGATGAAGA
  ACGAAAAATTAAAAGTGTGCATATGAGAGGTTTGGACAAGGAGACCTGTCTGATCCCAGCTGTGCAGGAG
  CCAATGTTCCCCCAGAGCTACAACCTTGTGACGGAGAACCTGGCCCTGACAGAGTATACCTTGTACTTGG
  GATTTCACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCG
  GGATGCTCAGCAGAGTTCTAGCCCTGCTGCTGACAATCTCCGTGAGCAGTTCGAACGCCTGATGACCATC
  TATCTCTCTACCAAGACTGCTATGACTGAACCCCAGATGCTGCAAAACTGTCTGAATTTGCAGGTGTCCA
  TGGCTGTTCTGCTGGTTCAACTGGCCATAGGCAATGAGGGCTCCCAGCCAATAGAACTAAGTTTCCCTTT
  GCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGATAACTTGGGTGATTTCCTCATT
  TTCCTCCGGCGCTTTGCTGAGGACATCTTGGAGACGTCAGCAGACTCCCTGGAGCATGTCCTTCATTTTA
  TCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCTCACCTGAGGGCCAAACTAGCAGAGGTGTT
  AGAAGCAGTGATGCCTCACCTAGACCAGACCCCAAGCCCCTTGGTGTCCAGTGTGTTCCACCGGAAACGT
  GTGTTCTGCAACTTTCCATACGCTCCCCAACTTGCAGAAGCTTTGATCAAGGTGTTTGTGGACATCGAGT
  TTACAGGGGACCCCCATCAATTTGAACAGAAGTTTAATTACCGCCGTCCTATGTATCCCATCCTAAGATA
  CATGTGGGGGACAGATTGCTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAA
  GCTATGAATCCCCCACTGTTCCTCCGATTTCTTAACCTGTTAATGAATGATGCTATCTTCCTTTTGGATG
  AAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAGATTGAGAAAGACCGAGGAGAATGGGAGAGTCT
  GACACCAGAAGCCCGCCGAGAGAAAGAGGCAGGTCTGCAGATGTTTGGGCAGCTGGCACGGTTCCATAAC
  ATCATGTCCAATGAAACAATTGGTACCCTTTCCTTCCTGACATCAGAGATCAAGTCGCTGTTTGTGCATC
  CGTTCCTGGCCGAGCGCATCATCTCCATGCTCAACTACTTCCTGCAGCACCTGGTGGGCCCCAAGATGGG
  TGCCCTCAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCTCAGCAGCTCGTATCAGACATCTGCACT
  ATCTACTTAAATCTTGGGTATTTGAGATTGGTGTTGAGAAAGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210961 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQAIFARLLLQDP
  GNHLISMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTP
  EIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALLLDEEVRTFPEVMIPVFDILLSRIKDLELCQ
  ILLYAYLDILLYFTRQKDMAKVFLEYIQPKDPSNGQMYQKTLLGVILNISCLLKTPGVVENHGFFLNPSR
  SSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILFWLGNCLHANAGRTKIWANQMPEIFFQ
  MYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCVLKDLNDEERKIKSVHMRGLDKETCLIPAVQE
  PMFPQSYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTI
  YLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELSFPLPDGYSSLAYVPEFFADNLGDFLI
  FLRRFAEDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPSPLVSSVFHRKR
  VFCNFPYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDCYRESIKDLADYASKNLE
  AMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWESLTPEARREKEAGLQMFGQLARFHN
  IMSNETIGTLSFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICT
  IYLNLGYLRLVLRKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145400
• ORF Size: 2568 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145400.2, NP_663375.2
• RefSeq Size: 6067 bp
• RefSeq ORF: 3087 bp
• Locus ID: 140630
• MW: 98.8 kDa