Eef2 (NM_007907) Mouse Tagged ORF Clone

CAT#: MR210973

Eef2 (Myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 2 (Eef2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eef2
• Synonyms: Ef-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210973 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAACTTCACAGTAGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCCGGAACATGTCAG
  TCATCGCCCATGTGGACCACGGCAAGTCCACGCTGACCGACTCCCTTGTGTGCAAGGCTGGCATCATTGC
  CTCTGCCCGAGCTGGGGAGACGCGCTTCACTGACACTCGCAAGGATGAGCAGGAGCGCTGCATCACAATC
  AAATCCACCGCCATCTCCCTCTTCTACGAGCTCTCTGAGAACGACCTGAACTTCATTAAGCAGAGCAAGG
  ATGGCTCGGGCTTCCTCATCAACCTCATCGACTCTCCAGGCCATGTGGACTTCTCTTCAGAGGTGACAGC
  TGCCTTGCGTGTCACCGATGGAGCTCTGGTGGTGGTGGACTGTGTGTCTGGCGTGTGTGTGCAGACAGAA
  ACTGTGCTGCGCCAGGCCATCGCTGAACGCATCAAGCCCGTCCTGATGATGAACAAGATGGACCGGGCCC
  TGCTGGAGCTGCAGCTGGAGCCCGAGGAGCTCTACCAGACCTTCCAGCGCATCGTGGAGAACGTCAACGT
  CATCATCTCTACCTACGGCGAGGGCGAGAGTGGGCCCATGGGCAATATCATGATTGACCCCGTCCTGGGC
  ACCGTAGGCTTTGGTTCTGGCCTGCATGGCTGGGCCTTCACCCTGAAGCAGTTTGCGGAGATGTATGTGG
  CCAAGTTTGCAGCCAAGGGTGAGGGCCAGCTGAGCGCAGCCGAGCGTGCCAAGAAAGTAGAGGACATGAT
  GAAGAAGCTGTGGGGAGACCGGTACTTTGATCCGGCCAATGGCAAGTTCAGTAAGTCAGCCAATAGCCCA
  GATGGGAAAAAACTTCCCCGCACCTTCTGCCAGCTCATCCTGGACCCCATCTTCAAGGTGTTCGACGCCA
  TCATGAACTTCAGGAAGGAGGAGACAGCCAAGCTGATCGAGAAGCTGGACATCAAGCTGGACAGCGAGGA
  CAAAGACAAGGAGGGCAAGCCATTGCTCAAGGCTGTGATGCGCCGCTGGCTGCCTGCAGGGGACGCCCTC
  CTGCAGATGATCACCATCCACTTACCATCCCCCGTCACTGCACAGAAGTACCGTTGTGAGCTGCTATACG
  AGGGGCCTCCCGACGATGAGGCCGCCATGGGTATTAAGAGCTGCGACCCCAAAGGCCCACTTATGATGTA
  CATTTCCAAGATGGTGCCAACCTCTGACAAAGGCCGCTTCTATGCCTTTGGTAGAGTGTTCTCTGGGGTG
  GTGTCCACGGGCCTGAAGGTCCGGATCATGGGCCCCAACTACACGCCTGGGAAGAAAGAGGACCTATACC
  TGAAGCCTATCCAGAGAACCATTCTGATGATGGGCCGCTACGTGGAGCCGATTGAGGACGTGCCATGTGG
  AAACATTGTGGGGCTGGTCGGAGTGGACCAGTTCCTTGTGAAGACGGGGACCATCACTACCTTTGAGCAC
  GCTCACAACATGCGCGTGATGAAGTTCAGCGTCAGCCCTGTCGTCCGTGTGGCAGTGGAGGCCAAGAACC
  CAGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAAGCGGCTGGCTAAGTCTGACCCTATGGTGCAGTGCAT
  CATTGAGGAGTCTGGAGAACATATTATTGCTGGCGCCGGTGAGCTGCACCTGGAGATCTGCCTTAAGGAC
  CTGGAGGAGGACCATGCCTGCATCCCCATCAAGAAATCTGACCCTGTTGTGTCATATCGGGAGACAGTCA
  GTGAGGAATCCAACGTGCTGTGTCTGTCCAAGTCCCCCAATAAGCACAACCGGCTGTACATGAAGGCCAG
  GCCCTTCCCTGATGGCCTGGCAGAGGACATCGATAAGGGTGAGGTGTCTGCCCGCCAGGAGCTCAAGGCA
  CGTGCCCGCTACCTGGCCGAAAAGTATGAGTGGGACGTTGCTGAAGCCCGCAAGATCTGGTGCTTTGGCC
  CTGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGA
  CAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAACATGCGTGGTGTG
  CGGTTTGATGTTCATGATGTGACCCTGCATGCTGATGCCATTCACCGGGGAGGTGGCCAGATCATCCCCA
  CAGCACGCCGCTGCCTGTATGCCAGTGTGCTGACCGCACAGCCCCGCCTCATGGAGCCTATCTATCTGGT
  GGAGATCCAGTGTCCTGAGCAAGTGGTGGGTGGCATCTACGGTGTCCTGAACAGGAAGCGTGGCCATGTG
  TTTGAGGAGTCCCAGGTGGCTGGTACCCCCATGTTTGTGGTCAAGGCATACCTGCCTGTCAATGAGTCCT
  TTGGCTTCACCGCTGATCTGCGATCCAACACCGGCGGCCAGGCCTTCCCCCAGTGCGTGTTTGACCACTG
  GCAGATCCTGCCTGGGGATCCTTTTGACAACAGCAGCCGCCCCAGCCAAGTGGTAGCTGAGACGCGCAAG
  CGCAAGGGCCTGAAAGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210973 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITI
  KSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTE
  TVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLG
  TVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLSAAERAKKVEDMMKKLWGDRYFDPANGKFSKSANSP
  DGKKLPRTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDAL
  LQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGV
  VSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEH
  AHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKD
  LEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKA
  RARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGV
  RFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHV
  FEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRK
  RKGLKEGIPALDNFLDKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007907
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007907.1, NM_007907.2, NP_031933.1
• RefSeq Size: 3126
• RefSeq ORF: 2577
• Locus ID: 13629
• MW: 95.3 kDa
• Gene Summary: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. [UniProtKB/Swiss-Prot Function]