Dis3l2 (NM_153530) Mouse Tagged ORF Clone

CAT#: MR211017

Dis3l2 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dis3l2
• Synonyms: 4930429A22Rik; 8030493P09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211017 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCATCCTGACTACAAGCTGAACCTTCGGTCTCCGGGGACCCCCAGAGGTGTGTCCTCTGTGGTTG
  GCCCGAGTGCTGTTGGTGCTTCGCCAGGTGACAAAAAGTCAAAGAACAAGTCCATGCGAGGGAAGAAAAA
  GAGCATATTTGAAACCTACATGTCCAAGGAGGATGTTTCAGAAGGCTTGAAGAGAGGAACACTTATCCAG
  GGTGTATTGAGAATCAACCCAAAGAAGTTTCATGAAGCCTTCATTCCTTCTCCGGATGGTGATCGGGACA
  TTTTTATTGATGGAGTTGTTGCTCGTAATAGAGCCTTAAATGGGGACCTTGTGGTTGTAAAACTGCTTCC
  TGAGGATCAGTGGAAGGCAGTTAAACCAGAGAGCAATGACAAAGAAATAGAAGCTACTTATGAAGCTGAC
  ATCCCTGAAGAGGGCTGTGGACATCACCCCCTGCAGCAGTCCCGGAAAGGCTGGAGTGGTCCTGATGTCA
  TTATAGAGGCTCAGTTTGATGACAGCGACTCAGAAGATAGACATGGCAACACCAGTGGCCTGGTTGATGG
  TGTTAAGAAATTGTCAATCTCTACTCCTGACAGAGGAAAAGAAGATTCTAGTACTCCAGTTATGAAAGAT
  GAGAACACCCCCATACCACAGGACACAAGAGGCTTATCAGAGAAGTCACTTCAGAAATCAGCAAAGGTGG
  TTTACATCTTGGAGAAAAAGCATTCTCGAGCAGCAACTGGCATCCTGAAACTCTTGGCTGATAAGAACAG
  TGACCTGTTTAAGAAATACGCCCTGTTTTCTCCTTCAGACCACCGAGTACCTAGAATTTACGTACCTCTC
  AAGGACTGTCCCCAGGACTTCATGACCCGACCTAAAGACTTTGCCAACACGCTGTTCATCTGCCGCATCA
  TAGACTGGAAGGAGGACTGTAATTTTGCCCTGGGGCAACTGGCTAAGAGTCTTGGGCAGGCTGGTGAAAT
  CGAGCCTGAAACAGAAGGGATACTGACAGAATATGGTGTGGACTTCTCTGATTTCTCTTCAGAAGTTCTT
  GAATGTCTCCCTCAAAGCCTGCCCTGGACAATCCCACCTGATGAGGTGGGCAAGAGAAGAGACCTAAGGA
  AAGACTGTATCTTCACCATTGATCCATCAACTGCTCGCGACCTTGATGATGCCCTCGCCTGCAGGCGGCT
  CACTGATGGCACCTTCGAAGTGGGCGTCCACATCGCCGATGTGAGTTACTTTGTTCCTGAGGGATCCTCT
  TTGGATAAAGTAGCTGCTGAGAGAGCCACAAGTGTCTACTTGGTCCAGAAGGTGGTCCCCATGCTTCCCA
  GGCTTCTGTGTGAGGAACTCTGCAGCCTCAACCCCATGACTGACAAGCTGACCTTCTCTGTGATCTGGAA
  GCTGACCCCTGAAGGCAAGATCCTTGAAGAGTGGTTTGGCCGCACTATCATCCGTTCTTGCACCAAACTG
  AGCTACGACCATGCCCAGAGCATGATCGAAAATCCAACTGAGAAGATCCCTGAGGAAGAGCTTCCCCCAA
  TTTCTCCAGAGCACAGCGTCGAGGAGGTGCACCAGGCAGTCCTGAACCTGCACAGCATTGCAAAGCAACT
  CCGCCGCCAGCGCTTTGTAGATGGCGCACTCCGTTTAGATCAGCTGAAGCTTGCTTTTACTCTGGACCAT
  GAGACTGGATTGCCTCAAGGATGTCACATCTATGAGTACCGAGACAGCAACAAGCTTGTAGAGGAGTTCA
  TGCTCCTGGCCAACATGGCGGTGGCCCACAAGATCTTCCGCACCTTCCCTGAGCAGGCCCTGCTGCGCCG
  GCATCCCCCACCACAGACGAAGATGCTCAGTGACCTGGTGGAGTTCTGTGACCAGATGGGGCTGCCCATG
  GATGTCAGCTCTGCAGGGGCCCTAAATAAAAGCCTGACTAAGACATTTGGAGATGACAAGTACTCTCTGG
  CCCGGAAGGAGGTGCTCACCAACATGTACTCCCGGCCCATGCAGATGGCACTGTACTTCTGCTCTGGGAT
  GCTGCAGGACCAGGAGCAGTTCCGGCATTATGCTCTCAACGTTCCCCTCTACACACACTTCACCTCTCCC
  ATCCGCCGCTTTGCTGACGTCATAGTGCACCGCCTCCTGGCTGCTGCTCTGGGCTACAGTGAACAGCCAG
  ATGTGGAGCCTGATACCCTACAGAAGCAAGCTGACCACTGCAATGACCGTCGCATGGCTTCCAAACGTGT
  GCAGGAGCTCAGCATCGGCCTCTTCTTCGCAGTTCTAGTAAAGGAGAGTGGCCCCCTGGAGTCCGAAGCC
  ATGGTGATGGGTGTCCTGAACCAAGCTTTCGACGTGCTGGTGCTGCGCTTTGGGGTGCAGAAGCGCATCT
  ACTGCAATGCACTGGCCCTGCGATCCTACAGCTTCCAGAAGGTGGGGAAGAAGCCAGAGCTCACTCTTGT
  TTGGGAGCCTGATGACCTTGAAGAGGAGCCAACACAGCAGGTCATCACCATCTTCAGCCTGGTGGATGTG
  GTCCTGCAGGCAGAGGCCACAGCCCTCAAGTACAGTGCTATCCTGAAGCGACCAGGCCTGGAGAAGGCGT
  CTGATGAGGAGCCTGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211017 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNHPDYKLNLRSPGTPRGVSSVVGPSAVGASPGDKKSKNKSMRGKKKSIFETYMSKEDVSEGLKRGTLIQ
  GVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEDQWKAVKPESNDKEIEATYEAD
  IPEEGCGHHPLQQSRKGWSGPDVIIEAQFDDSDSEDRHGNTSGLVDGVKKLSISTPDRGKEDSSTPVMKD
  ENTPIPQDTRGLSEKSLQKSAKVVYILEKKHSRAATGILKLLADKNSDLFKKYALFSPSDHRVPRIYVPL
  KDCPQDFMTRPKDFANTLFICRIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVL
  ECLPQSLPWTIPPDEVGKRRDLRKDCIFTIDPSTARDLDDALACRRLTDGTFEVGVHIADVSYFVPEGSS
  LDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILEEWFGRTIIRSCTKL
  SYDHAQSMIENPTEKIPEEELPPISPEHSVEEVHQAVLNLHSIAKQLRRQRFVDGALRLDQLKLAFTLDH
  ETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRTFPEQALLRRHPPPQTKMLSDLVEFCDQMGLPM
  DVSSAGALNKSLTKTFGDDKYSLARKEVLTNMYSRPMQMALYFCSGMLQDQEQFRHYALNVPLYTHFTSP
  IRRFADVIVHRLLAAALGYSEQPDVEPDTLQKQADHCNDRRMASKRVQELSIGLFFAVLVKESGPLESEA
  MVMGVLNQAFDVLVLRFGVQKRIYCNALALRSYSFQKVGKKPELTLVWEPDDLEEEPTQQVITIFSLVDV
  VLQAEATALKYSAILKRPGLEKASDEEPED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153530
• ORF Size: 2613 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153530.1, NM_153530.2, NP_705758.1
• RefSeq Size: 3129 bp
• RefSeq ORF: 2613 bp
• Locus ID: 208718
• MW: 97.8 kDa