Tyro3 (BC082325) Mouse Tagged ORF Clone

CAT#: MR211050

Tyro3 (Myc-DDK-tagged) - Mouse TYRO3 protein tyrosine kinase 3 (cDNA clone MGC:90668 IMAGE:30356080)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tyro3
• Synonyms: Dtk, Rse, Sky, Tif, Etk-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211050 representing BC082325.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGCTGAGGCGGAGCATGGGGTGGCCGGGGCTCCGGCCGCTGCTGCTGGCGGGACTGGCTTCTCTG
  CTGCTCCCCGGGTCTGCGGCCGCAGGCCTGAAGCTCATGGGCGCCCCAGTGAAGATGACCGTGTCTCAG
  GGGCAGCCAGTGAAGCTCAACTGCAGCGTGGAGGGGATGGAGGACCCTGACATCCACTGGATGAAGGAT
  GGCACCGTGGTCCAGAATGCAAGCCAGGTGTCCATCTCCATCAGCGAGCACAGCTGGATTGGCTTACTC
  AGCCTAAAGTCAGTGGAACGGTCTGATGCTGGCCTGTACTGGTGCCAGGTGAAGGATGGGGAGGAAACC
  AAGATTTCTCAGTCAGTATGGCTCACTGTCGAAGGTGTGCCATTCTTCACAGTGGAACCAAAAGATCTG
  GCGGTGCCACCCAATGCCCCTTTTCAGCTGTCTTGTGAGGCTGTGGGTCCTCCAGAACCCGTAACCATT
  TACTGGTGGAGAGGACTCACTAAGGTTGGGGGACCTGCTCCCTCTCCCTCTGTTTTAAATGTGACAGGA
  GTGACCCAGCGCACAGAGTTTTCTTGTGAAGCCCGCAACATAAAAGGCCTGGCCACTTCCCGACCAGCC
  ATTGTTCGCCTTCAAGCACCGCCTGCAGCTCCTTTCAACACCACAGTAACAACGATCTCCAGCTACAAC
  GCTAGCGTGGCCTGGGTGCCAGGTGCTGACGGCCTAGCTCTGCTGCATTCCTGTACTGTACAGGTGGCA
  CACGCCCCAGGAGAATGGGAGGCCCTTGCTGTTGTGGTTCCTGTGCCACCTTTTACCTGCCTGCTTCGG
  AACTTGGCCCCTGCCACCAACTACAGCCTTAGGGTGCGCTGTGCCAATGCCTTGGGCCCTTCTCCCTAC
  GGCGACTGGGTGCCCTTTCAGACAAAGGGCCTAGCGCCAGCCAGAGCTCCTCAGAATTTCCATGCCATT
  CGTACCGACTCAGGCCTTATCCTGGAATGGGAAGAAGTGATTCCTGAGGACCCTGGGGAAGGCCCCCTA
  GGACCTTATAAGCTGTCCTGGGTCCAAGAAAATGGAACCCAGGATGAGCTGATGGTGGAAGGGACCAGG
  GCCAATCTGACCGACTGGGATCCCCAGAAGGACCTGATTTTGCGTGTGTGTGCCTCCAATGCAATTGGT
  GATGGGCCCTGGAGTCAGCCACTGGTGGTGTCTTCTCATGACCATGCAGGGAGGCAGGGCCCTCCCCAC
  AGCCGCACATCCTGGGTGCCTGTGGTCCTGGGCGTGCTCACCGCCCTGATCACAGCTGCTGCCTTGGCC
  CTCATCCTGCTTCGGAAGAGACGCAAGGAGACGCGTTTCGGGCAAGCCTTTGACAGTGTCATGGCCCGA
  GGGGAGCCAGCTGTACACTTCCGGGCAGCCCGATCTTTCAATCGAGAAAGGCCTGAACGCATTGAGGCC
  ACATTGGATAGCCTGGGCATCAGCGATGAATTGAAGGAAAAGCTGGAGGATGTCCTCATTCCAGAGCAG
  CAGTTCACCCTCGGTCGGATGTTGGGCAAAGGAGAGTTTGGATCAGTGCGGGAAGCCCAGCTAAAGCAG
  GAAGATGGCTCCTTCGTGAAAGTGGCAGTGAAGATGCTGAAAGCTGACATCATTGCCTCAAGCGACATA
  GAAGAGTTCCTCCGGGAAGCAGCTTGCATGAAGGAGTTTGACCATCCACACGTGGCCAAGCTTGTTGGG
  GTGAGCCTCCGGAGCAGGGCTAAAGGTCGTCTCCCCATTCCCATGGTCATCCTGCCCTTCATGAAACAT
  GGAGACTTGCACGCCTTTCTGCTCGCCTCCCGAATCGGGGAGAACCCTTTTAACCTGCCCCTCCAGACC
  CTGGTCCGGTTCATGGTGGACATTGCCTGTGGCATGGAGTACCTGAGCTCCCGGAACTTCATCCACCGA
  GACCTAGCAGCTCGGAATTGCATGCTGGCCGAGGACATGACAGTGTGTGTGGCTGATTTTGGACTCTCT
  CGGAAAATCTATAGCGGGGACTATTATCGTCAGGGCTGTGCCTCCAAATTGCCCGTCAAGTGGCTGGCC
  CTGGAGAGCTTGGCTGACAACTTGTATACTGTACACAGTGATGTGTGGGCCTTCGGGGTGACCATGTGG
  GAGATCATGACTCGTGGGCAGACGCCATATGCTGGCATTGAAAATGCCGAGATTTACAACTACCTCATC
  GGCGGGAACCGCCTGAAGCAGCCTCCGGAGTGCATGGAGGAAGTGTATGATCTCATGTACCAGTGCTGG
  AGCGCCGACCCCAAGCAGCGCCCAAGCTTCACGTGTCTGCGAATGGAACTGGAGAACATTCTGGGCCAC
  CTGTCTGTGCTGTCCACCAGCCAGGACCCCTTGTACATCAACATTGAGAGAGCTGAGCAGCCTACTGAG
  AGTGGCAGCCCTGAGGTCCACTGTGGAGAGCGATCCAGCAGCGAGGCAGGGGACGGCAGTGGCGTGGGG
  GCAGTAGGTGGCATCCCCAGTGACTCTCGGTACATCTTCAGCCCCGGAGGGCTATCCGAGTCACCAGGG
  CAGCTGGAGCAGCAGCCAGAAAGCCCCCTCAATGAGAACCAGAGGCTGTTGTTGCTGCAGCAAGGGCTA
  CTGCCTCACAGTAGCTGT
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR211050
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MALRRSMGWPGLRPLLLAGLASLLLPGSAAAGLKLMGAPVKMTVSQGQPVKLNCSVEGMEDPDIHWMKD
  GTVVQNASQVSISISEHSWIGLLSLKSVERSDAGLYWCQVKDGEETKISQSVWLTVEGVPFFTVEPKDL
  AVPPNAPFQLSCEAVGPPEPVTIYWWRGLTKVGGPAPSPSVLNVTGVTQRTEFSCEARNIKGLATSRPA
  IVRLQAPPAAPFNTTVTTISSYNASVAWVPGADGLALLHSCTVQVAHAPGEWEALAVVVPVPPFTCLLR
  NLAPATNYSLRVRCANALGPSPYGDWVPFQTKGLAPARAPQNFHAIRTDSGLILEWEEVIPEDPGEGPL
  GPYKLSWVQENGTQDELMVEGTRANLTDWDPQKDLILRVCASNAIGDGPWSQPLVVSSHDHAGRQGPPH
  SRTSWVPVVLGVLTALITAAALALILLRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEA
  TLDSLGISDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDGSFVKVAVKMLKADIIASSDI
  EEFLREAACMKEFDHPHVAKLVGVSLRSRAKGRLPIPMVILPFMKHGDLHAFLLASRIGENPFNLPLQT
  LVRFMVDIACGMEYLSSRNFIHRDLAARNCMLAEDMTVCVADFGLSRKIYSGDYYRQGCASKLPVKWLA
  LESLADNLYTVHSDVWAFGVTMWEIMTRGQTPYAGIENAEIYNYLIGGNRLKQPPECMEEVYDLMYQCW
  SADPKQRPSFTCLRMELENILGHLSVLSTSQDPLYINIERAEQPTESGSPEVHCGERSSSEAGDGSGVG
  AVGGIPSDSRYIFSPGGLSESPGQLEQQPESPLNENQRLLLLQQGLLPHSSC
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using MR211050 also available, TP511050
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC082325
• ORF Size: 2640 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3960 bp
• RefSeq ORF: 2642 bp
• Locus ID: 22174
• Cytogenetics: 2 59.97 cM
• MW: 96.2 kDa
• Gene Summary: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.[UniProtKB/Swiss-Prot Function]