Pcdha1 (BC082326) Mouse Tagged ORF Clone

CAT#: MR211110

Pcdha1 (Myc-DDK-tagged) - Mouse protocadherin alpha 1 (cDNA clone MGC:90628 IMAGE:30356350)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pcdha1
• Synonyms: CNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211110 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTTTCCAGGCTCCGAGGCCTGAGAGCCCGGCGCCTGCTCTTGTTCTCGCTTCTGTTTCTTGCAG
  CCTGGGAGGCAGGGAGCGGCCAGATTCATTACTCTGTCCCAGAGGAAGCCAAACACGGCACCTTTGTGGG
  CCGCATCGCGCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTATTCAGGGTGGCGTCCAAG
  GATCGCGGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGG
  AGGAGCTGTGCGGGCGGAGCGCGGAGTGTAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGT
  TTTCCACGTGGAGGTGAAGGTTAAAGATATTAATGATAACCCACCAGTTTTCAAAGGCGCAGAGCAAAGG
  ATATTTATTCCTGAAAACAGACAACTAGATTCACGGTTTCCACTAGAGGGCGCCGTGGACGCAGACATTG
  GTGCCAATTCTCTTTTAACTTACACGCTGAGCCCCACTGATTACTTTTCTTTAAAGGTAGAGACAACAGA
  CGAACTCAGTAAATCCCTTTCGCTTGAACTGAGAAAATCTTTGGATAGAGAAGAAACACCAGAACTTCAG
  TTATTACTGACAGCCACCGACGGGGGCAAGCCAGAGCTGGAGGGGACAGTTCGGCTGCAGATTACTGTGC
  TCGATGTTAATGACAACGCCCCACTGTTTGACCAGGCCATCTACAGAGCACAGTTAGTAGAATCTACAGT
  GAATGGGACATTAGTGACGACGTTAAATGCCACTGACGCTGATGAAGGTGTTAATGGTGAAGTTGTGTTT
  TCCTTTGGCAATGACGTTTCTCTTGACATTCAAGAAAAATTCAATGTGGATTCTCTTTCAGGAGAAATTA
  GAGTGATCGGTGATCTAGATTATGAAAAAACAAAATCCTATGAAATTCAAATAAAGGCAGTTGATAAAGG
  GACTCCCTCCATGTCAAATCACTGCAAGGTTTTGGTGAAAGTACTGGATATAAATGATAATGCTCCAGAG
  CTGTCGATCACATCCTTGTCTTTGCCCATCAAAGAGGATACTCCACTTAACACTATCATCGCACTCATCA
  AGGTGTCCGACATCGACTCAGGTGTCAACGGGCAGGTGACCTGCTCCCTAACTCCTCATGTCCCTTTCAA
  GTTGGTGTCCACCTTCAAGAATTACTATTCGCTTGTGCTGGACAGCACCCTGGACCGAGAGACCACAGCG
  GACTATAAGGTGGTGGTGACAGCCCGCGACGGGGGCTCGCCCTCGCTGTGGGCCACGGCCAGCGTGTCTG
  TGGAGGTGGCTGACGTGAACGACAATGCTCCAGTGTTCGCGCAGCCCGAATACACTGTGTTCGTGAAGGA
  GAACAACCCGCCTGGTGCGCACATCTTCACGGTGTCAGCGATGGACGCGGATGCGCAGGAGAATGCGCTG
  GTGTCCTACTCGCTGGTGGAGCGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGTGTCTGTGCACGCGG
  AGAGCGGCAAGGTGTTCGCGCTGCAGCCTCTGGACCATGAGGAGCTGGAGCTGCTGCAGTTCCAGGTGAG
  CGCGCGGGATGCTGGTGTGCCTGCCCTGGGCAGCAATGTGACTCTGCAGGTGTTTGTGCTGGACGAGAAT
  GACAATCCACCCACACTGCTGGGGCCTCACGCAGGCAGCGCAGTGAGCGAACTGGTGTCACGGACAATGG
  GTGCAGGGCATGTGGTGACAAAGGTGCGCGCGGTGGACGCGGACTCTGGCTACAATGCGTGGCTGTCCTA
  TGAACTACAACCTCCTACAGGTGGTGCGCGCAACCCGTTTCGCGTGGGTCTGTACACTGGTGAGATTAGC
  ACCACACGCTCCTTAGATGAGGTCGATGTTCCACGACAGCGTCTCTTGGTGCTGGTGAAGGACCATGGAG
  AGCCAATGCTGACTGCTACCGCTACCGTGTTGGTATCTCTGGTGGAAAGCAGTCCGATTCAGAAAGCCTC
  TTTGCCTGTATTAACCGGTGCAGTGGGTTCCAAGGCATCGCTGGTGGATGTCAACGTGTACCTGATCATC
  GCCATCTGTGCCGTGTCCAGCCTGTTAGTGCTCACTTTGCTTCTGTACACAGCGCTGCGCTGCTCCGCGA
  CACCCACTGATGGAGCCTGTGGTCCGGGGAAGCCCATGCTGGTGTGCTCCAGCGCGGTGGGGAGCTGGTC
  ATACTCGCAGCAGAGGCGACAGAAGGTGTGCTCTGGAGAGGGTCCACCTAAGACCGACCTCATGGCCTTC
  AGCCCGGGTTTAGCCCCAGGTTTGAACATAGCGGAAAGAAGTGACCACGCAGAAGTAAATTCAGATCTTT
  CTGGTAATCCCCGGCAGCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAAGAGCAGGCATGCACAGCTC
  TGTGCACCTGGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTA
  TCCAGTGCAACACCAGAACCTGAGGCAGGAGAGGTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACA
  GCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAGTCCGGTCCCGAACCTAAAAAGCAGACCCAGGT
  TTCCTTTCTCCCCCGCCGCAAAGGAGAAGCTTCCCAGCCCCGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211110 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLFSRLRGLRARRLLLFSLLFLAAWEAGSGQIHYSVPEEAKHGTFVGRIAQDLGLELTELVPRLFRVASK
  DRGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVKVKDINDNPPVFKGAEQR
  IFIPENRQLDSRFPLEGAVDADIGANSLLTYTLSPTDYFSLKVETTDELSKSLSLELRKSLDREETPELQ
  LLLTATDGGKPELEGTVRLQITVLDVNDNAPLFDQAIYRAQLVESTVNGTLVTTLNATDADEGVNGEVVF
  SFGNDVSLDIQEKFNVDSLSGEIRVIGDLDYEKTKSYEIQIKAVDKGTPSMSNHCKVLVKVLDINDNAPE
  LSITSLSLPIKEDTPLNTIIALIKVSDIDSGVNGQVTCSLTPHVPFKLVSTFKNYYSLVLDSTLDRETTA
  DYKVVVTARDGGSPSLWATASVSVEVADVNDNAPVFAQPEYTVFVKENNPPGAHIFTVSAMDADAQENAL
  VSYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLQFQVSARDAGVPALGSNVTLQVFVLDEN
  DNPPTLLGPHAGSAVSELVSRTMGAGHVVTKVRAVDADSGYNAWLSYELQPPTGGARNPFRVGLYTGEIS
  TTRSLDEVDVPRQRLLVLVKDHGEPMLTATATVLVSLVESSPIQKASLPVLTGAVGSKASLVDVNVYLII
  AICAVSSLLVLTLLLYTALRCSATPTDGACGPGKPMLVCSSAVGSWSYSQQRRQKVCSGEGPPKTDLMAF
  SPGLAPGLNIAERSDHAEVNSDLSGNPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTV
  SSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPEPKKQTQVSFLPRRKGEASQPRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC082326
• ORF Size: 2706 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082326, AAH82326
• RefSeq Size: 4942 bp
• RefSeq ORF: 2708 bp
• Locus ID: 116731
• MW: 97.5 kDa