Ctnna1 (NM_009818) Mouse Tagged ORF Clone

CAT#: MR211134

  • TrueORF®

Ctnna1 (Myc-DDK-tagged) - Mouse catenin (cadherin associated protein), alpha 1 (Ctnna1)


  "NM_009818" in other vectors (4)


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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ctnna1
Synonyms 2010010M04Rik; AA517462; AI988031; Catna1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211134 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGCCGTCCACGCAGGCAACATAAACTTCAAATGGGACCCCAAAAGTCTGGAGATTAGGACTCTGG
CTGTTGAAAGACTGTTGGAGCCTCTTGTTACACAGGTTACAACCCTGGTAACCACCAATAGTAAAGGGCC
GTCTAATAAAAAGAGAGGCCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAACTGAG
AATTTCTTGGAAAAGGGGGATAAAATTGCAAAAGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGTTGCTG
TAGAAGATGTTCGAAAACAAGGAGATTTGATGAAGAGCGCTGCTGGGGAGTTTGCAGATGATCCATGCTC
TTCTGTGAAGCGAGGCAACATGGTCCGGGCAGCTCGAGCTTTGCTCTCTGCCGTCACCCGGCTGCTGATT
CTGGCTGACATGGCAGATGTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATATTGAAAC
TGAGGAATGCTGGCAATGAACAAGACTTAGGGATACAGTACAAAGCTCTGAAACCAGAAGTAGATAAGCT
GAACATCATGGCAGCAAAAAGACAACAGGAACTAAAAGATGTGGGCAATCGTGATCAGATGGCTGCAGCT
AGAGGAATCCTGCAGAAAAATGTTCCAATCCTCTATACTGCATCCCAGGCATGTCTACAACACCCTGATG
TTGCAGCCTATAAGGCCAACCGGGACTTGATATACAAGCAGCTGCAGCAGGCCGTCACAGGAATTTCTAA
TGCAGCCCAGGCTACTGCATCAGATGATGCTGCCCAGCACCAGGGTGGCAGTGGCGGAGAGCTGGCATAC
GCTCTCAACAACTTTGATAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCGT
CCCTGGAGGAGCGCCTGGAAAGCATTATTAGTGGGGCTGCCCTGATGGCTGACTCATCCTGCACACGTGA
TGACCGTCGGGAGCGCATTGTGGCAGAGTGTAATGCTGTCCGCCAGGCTCTACAGGACCTGCTCTCAGAA
TACATGGGCAACGCTGGACGTAAAGAAAGAAGTGATGCACTCAACTCTGCAATAGATAAAATGACCAAGA
AGACCAGGGACTTGCGTAGACAGCTTCGCAAAGCTGTCATGGACCACGTATCAGATTCTTTCCTGGAGAC
CAATGTCCCTCTATTAGTACTGATTGAAGCTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAATATGCC
CAAGTTTTTCGTGAACATGCCAACAAACTGATTGAGGTTGCCAACCTGGCCTGTTCAATCTCCAACAATG
AAGAAGGCGTGAAGCTTGTCCGAATGTCTGCAAGCCAGTTAGAAGCGCTGTGTCCTCAGGTTATCAATGC
TGCATTGGCGTTGGCAGCAAAGCCGCAGAGTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAGCAA
TGGGAAAAGCAAGTCCGTGTTCTCACAGATGCTGTTGATGACATTACTTCCATCGATGACTTCTTGGCTG
TCTCAGAGAACCACATTTTAGAAGATGTGAACAAGTGTGTTATTGCTCTCCAAGAGAAAGACGTGGATGG
GCTGGATCGCACTGCCGGTGCCATCCGAGGCCGGGCAGCCCGAGTCATTCATGTAGTCACCTCAGAGATG
GACAACTACGAGCCAGGAGTTTACACAGAGAAGGTTCTGGAAGCCACCAAGCTCCTCTCCAACACAGTCA
TGCCGCGCTTCACTGAGCAGGTGGAAGCAGCTGTGGAAGCCCTCAGCTCGGACCCGGCCCAGCCCATGGA
TGAGAATGAGTTCATCGACGCCTCCCGTCTGGTGTACGACGGCATCCGGGACATCCGGAAAGCAGTGCTG
ATGATCAGGACCCCCGAGGAGTTGGACGACTCTGACTTCGAGACTGAAGACTTTGATGTCAGAAGCAGGA
CCAGTGTCCAGACAGAAGATGACCAGCTGATAGCTGGCCAGAGCGCCCGGGCGATCATGGCTCAGCTTCC
TCAGGAGCAAAAAGCAAAGATTGCAGAACAGGTGGCCAGTTTCCAGGAAGAGAAGAGCAAGCTGGATGCT
GAAGTGTCCAAGTGGGATGACAGTGGCAATGACATCACTGTGCTGGCCAAGCAGATGTGCATGATCATGA
TGGAGATGACCGACTTCACCCGAGGCAAAGGTCCACTCAAAAACACCTCTGACGTGATCAGTGCTGCCAA
GAAAATTGCGGAGGCGGGATCCAGGATGGACAAGCTCGGCCGGACCATCGCAGACCATTGCCCAGACTCG
GTATGCAAGCAGGACCTGCTGGCCTACCTTCAGCGCATCGCTCTCTACTGCCACCAGCTCAACATCTGCA
GCAAAGTCAAGGCTGAAGTACAGAACCTTGGTGGAGAGCTGGTCGTCTCTGGGGTGGACAGCGCCATGTC
CCTGATCCAGGCGGCCAAGAACTTGATGAATGCTGTTGTGCAGACAGTGAAGGCGTCCTACGTGGCTTCC
ACCAAATACCAGAAGTCACAGGGGATGGCTTCCTTGAACCTTCCTGCTGTGTCATGGAAGATGAAGGCCC
CTGAGAAGAAGCCGTTGGTGAAGAGAGAGAAGCAGGATGAGACGCAGACCAAGATTAAACGAGCTTCTCA
GAAGAAACACGTGAACCCAGTGCAGGCCCTGAGCGAGTTCAAAGCCATGGACAGCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211134 protein sequence
Red=Cloning site Green=Tags(s)

MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVTTNSKGPSNKKRGRSKKAHVLAASVEQATE
NFLEKGDKIAKESQFLKEELVVAVEDVRKQGDLMKSAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLI
LADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGNRDQMAAA
RGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDAAQHQGGSGGELAY
ALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSE
YMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYA
QVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQ
WEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEM
DNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVL
MIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDA
EVSKWDDSGNDITVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDS
VCKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVAS
TKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009818
ORF Size 2721 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009818.1, NP_033948.1
RefSeq Size 3713 bp
RefSeq ORF 2721 bp
Locus ID 12385
MW 100.1 kDa

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