Psmd2 (NM_134101) Mouse Tagged ORF Clone

CAT#: MR211143

Psmd2 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (Psmd2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psmd2
• Synonyms: 9430095H01Rik; AA407121; TEG-190; Tex190
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211143 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGGTGGACGAGACAAGACACCCGTGCAGTCCCAACAGCCCTCCGCGACAACCCCCAGCGGCG
  CGGACGAGAAGTCGAGTGGCAAGGAGCGAAGGGATGCCGGGGAAAAAGACAAAGAGCAGGAGCTGTCTGA
  GGAGGACAAACAGCTTCAAGATGAACTGGAGATGCTCGTGGAGCGACTCGGGGAGAAAGATACATCTCTA
  TATCGACCAGCTCTGGAGGAACTGAGAAGACAAATTCGTTCTTCTACAACTTCCATGACTTCAGTACCCA
  AGCCTCTCAAATTTCTGCGTCCACACTATGGCAAATTGAAGGAAATCTATGAGAACATGGCCCCTGGGGA
  GAATAAGTGTTTTGCAGCTGACATCATCTCTGTTTTGGCCATGACCATGAGTGGTGAGCGGGAGTGCCTC
  AAGTATCGGCTCGTGGGCTCCCAGGAAGAACTGGCGTCATGGGGCCATGAATACGTCAGACATCTGGCAG
  GAGAGGTGGCTAAGGAGTGGCAGGAGCTGGATGATGCGGAGAAAGCACAGCGGGAACCACTGCTTACCTT
  GGTAAAGGAAATCGTCCCCTACAACATGGCTCACAATGCAGAGCACGAGGCTTGTGACCTGCTGATGGAA
  ATTGAGCAGGTGGACATGCTAGAGAAGGACATTGATGAGAACGCTTACGCCAAGGTCTGCCTCTATCTCA
  CCAGTTGTGTGAATTATGTACCAGAACCTGAGAACTCTGCCCTCCTGCGCTGCGCCTTGGGCGTGTTCCG
  AAAGTTTAGCCGTTTCCCTGAAGCTCTGAGATTGGCACTGATGCTCAATGACATGGAACTGGTAGAAGAC
  ATTTTCACTTCCTGCAAAGATGTGGTGGTCCAGAAGCAGATGGCATTCATGCTGGGTCGGCATGGAGTGT
  TCCTGGAGCTGAGTGAAGATGTAGAGGAATATGAAGACCTCACAGAAATCATGTCCAATGTCCAGCTCAA
  CAGCAACTTCTTGGCTTTAGCGCGGGAGCTGGACATCATGGAGCCCAAGGTGCCTGATGACATCTACAAA
  ACCCACCTAGAGAATAACAGGTTTGGTGGCAGTGGCTCTCAAGTGGATTCTGCACGAATGAACCTAGCCT
  CCTCTTTTGTAAATGGCTTTGTGAATGCAGCCTTTGGTCAAGATAAACTACTGACTGATGATGGCAACAA
  ATGGCTTTACAAGAACAAGGACCATGGGATGCTAAGTGCTGCTGCGTCTCTTGGCATGATTCTGCTGTGG
  GATGTGGATGGTGGCCTCACCCAGATTGACAAGTATCTGTACTCCTCTGAGGACTACATCAAGTCAGGAG
  CTCTCCTTGCCTGTGGTATCGTGAATTCTGGAGTCCGGAATGAGTGTGATCCTGCCCTGGCACTGCTTTC
  AGACTATGTTCTCCATAACAGCAATACCATGAGACTTGGCTCCATCTTTGGGCTAGGTTTGGCCTATGCT
  GGCTCTAATCGGGAAGATGTTCTAACACTGCTGCTACCTGTAATGGGAGATTCCAAGTCCAGCATGGAGG
  TGGCAGGTGTGACGGCTCTAGCTTGTGGGATGATAGCAGTGGGGTCCTGCAATGGAGATGTCACTTCCAC
  CATCCTTCAGACCATCATGGAGAAATCTGAGACTGAGCTCAAGGATACCTATGCTCGTTGGCTTCCTCTT
  GGCCTGGGCCTCAATCACTTGGGGAAAGGCGAAGCTATTGAGGCCATCCTGGCTGCCTTGGAGGTGGTGT
  CAGAGCCATTCCGCAGTTTTGCCAATACCCTGGTGGATGTATGTGCCTATGCAGGCTCTGGGAATGTGCT
  GAAGGTTCAGCAGCTCCTCCACATCTGTAGTGAGCACTTTGACTCTAAGGAAAAGGAGGAAGACAAAGAC
  AAGAAGGAAAAGAAGGACAAGGACAAGAAGGAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTAGCTG
  TTCTGGGCATTGCACTTATTGCTATGGGGGAAGAGATTGGTGCAGAGATGGCATTGCGAACTTTTGGTCA
  CTTGCTGAGATATGGGGAGCCTACCCTCCGTCGCGCTGTACCTTTAGCACTGGCACTAATTTCTGTTTCA
  AATCCACGACTCAACATCCTGGATACGCTAAGCAAATTCTCTCATGATGCTGATCCAGAAGTTTCCTATA
  ACTCCATCTTTGCTATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGTCTGGCTGCAATGTTGCGCCA
  GTTAGCTCAATATCATGCCAAGGACCCCAATAACCTCTTCATGGTGCGCTTGGCACAGGGCTTGACACAT
  TTAGGGAAGGGCACACTTACCCTCTGCCCCTATCACAGTGATCGGCAGCTCATGAGTCAAGTAGCGGTGG
  CAGGGCTACTCACGGTGCTTGTTTCTTTCCTGGATGTTCGAAACATCATCCTAGGCAAGTCTCACTATGT
  CCTGTATGGGCTGGTAGCCGCCATGCAGCCTCGGATGCTGGTTACATTTGATGAGGAGCTGCGACCATTG
  CCAGTTTCTGTCCGTGTGGGTCAGGCAGTGGATGTGGTGGGCCAGGCTGGGAAGCCAAAAACAATCACAG
  GGTTCCAGACTCATACAACGCCAGTGTTGCTGGCCCATGGGGAGCGGGCAGAGTTGGCCACTGAGGAGTT
  TCTTCCTGTCACCCCCATTCTGGAAGGCTTTGTTATTCTTCGGAAGAACCCCAACTATGATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211143 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGGRDKTPVQSQQPSATTPSGADEKSSGKERRDAGEKDKEQELSEEDKQLQDELEMLVERLGEKDTSL
  YRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKCFAADIISVLAMTMSGERECL
  KYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKAQREPLLTLVKEIVPYNMAHNAEHEACDLLME
  IEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVED
  IFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYK
  THLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLW
  DVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYA
  GSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPL
  GLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKD
  KKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVS
  NPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTH
  LGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPL
  PVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134101
• ORF Size: 2727 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134101.1, NM_134101.2, NP_598862.1
• RefSeq Size: 3000 bp
• RefSeq ORF: 2727 bp
• Locus ID: 21762
• MW: 100.2 kDa