Ano6 (NM_175344) Mouse Tagged ORF Clone

CAT#: MR211153

Ano6 (Myc-DDK-tagged) - Mouse anoctamin 6 (Ano6)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ano6
• Synonyms: 2900059G15Rik; AA407480; AW554778; F730003B03Rik; Tmem16f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211153 representing NM_175344.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGATGATGACTAGGAAGGTCCTGCTGAACATGGAGCTGGAGGAGGACGACGATGAGGATGGAGAC
  ATTGTGCTGGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGAC
  TTCAGGACTCCAGAGTTTGAAGAATTTAACGGGAAGCCCGACTCCCTCTTTTTCACCGATGGCCAGAGG
  CGAATCGACTTCATCCTCGTGTATGAAGATGAGAGCAAAAAGGAGAACAATAAGAAAGGGACAAATGAG
  AAACAGAAGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGCCATGGGCTGCAGCTGGAAGCAACA
  AGATCTGTTTCTGATGACAAGCTTGTGTTCGTAAAAGTGCACGCGCCCTGGGAAGTGCTGTGCACCTAT
  GCTGAGATCATGCACATCAAACTCCCGCTAAAGCCAAACGACCTGAAAACGCGCTCGCCCTTTGGCAAC
  CTCAACTGGTTCACCAAGGTCCTCCGGGTGAACGAGAGTGTCATCAAGCCAGAGCAGGAGTTCTTCACT
  GCCCCTTTTGAGAAGAGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCC
  ACCAGAAGCCGCATTGTTTATTTCATCCTCTCTCGGGTCAAATACCAAGTGATGAACAACGTTAACAAA
  TTTGGGATTAATAGACTGGTCAGCTCTGGAATCTACAAAGCAGCGTTTCCTCTGCACGACTGCAGATTC
  AACTATGAGTCGGAGGACATCAGTTGTCCTAGCGAGCGTTACCTCCTGTACAGAGAATGGGCTCACCCT
  CGGAGTATATACAAGAAGCAGCCCTTGGATCTTATCAGGAAGTATTACGGCGAGAAGATTGGAATCTAC
  TTTGCTTGGCTGGGCTATTACACGCAGATGCTCCTTCTCGCAGCTGTGGTGGGCGTGGCCTGCTTCCTC
  TATGGATATCTTGATCAGGATAACTGCACTTGGAGCAAAGAGGTCTGTGATCCTGACATCGGTGGCCAG
  ATCCTGATGTGCCCCCAGTGTGACAGGCTGTGTCCGTTCTGGAGGCTGAATATCACCTGTGAATCTTCT
  AAGAAATTGTGCATCTTTGACAGTTTTGGAACCCTGATCTTCGCTGTATTTATGGGAGTGTGGGTTACA
  TTGTTTTTGGAGTTTTGGAAGCGGCGCCAGGCAGAGCTCGAATATGAATGGGACACCGTTGAGCTACAG
  CAAGAGGAACAGGCCCGGCCAGAGTATGAGGCCCAGTGCAATCACGTGGTGATAAACGAGATCACTCAG
  GAGGAGGAGCGCATCCCATTTACCACCTGTGGGAAGTGCATCCGGGTCACGCTGTGTGCGAGCGCCGTC
  TTCTTCTGGATCCTGCTCATCATCGCCTCTGTGATCGGGATCATTGTCTACAGGCTGTCCGTGTTCATC
  GTATTTTCCACAACGCTCCCCAAGAACCCCAACGGGACAGACCCGATCCAGAAGTACCTGACCCCACAG
  ATGGCCACATCCATCACAGCCTCCATCATCAGCTTCATCATCATCATGATCCTCAACACGATCTACGAG
  AAGGTGGCCATCATGATCACCAACTTCGAGCTCCCAAGGACCCAGACGGATTATGAGAACAGCCTGACC
  ATGAAGATGTTCTTGTTCCAGTTTGTCAACTATTACTCCTCATGCTTCTACATCGCATTCTTCAAGGGC
  AAATTCGTGGGCTATCCCGGGGACCCAGTGTACTTGCTGGGCAAATACAGAAGCGAAGAGTGTGACCCG
  GGGGGCTGCCTCCTTGAACTGACCACACAGCTGACGATCATCATGGGGGGAAAGGCAATCTGGAACAAC
  ATACAAGAAGTCTTGCTCCCATGGGTTATGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAA
  AAGATAACCCCACGATGGGAACAGGATTACCATCTGCAGCCCATGGGCAAGCTGGGATTGTTCTATGAG
  TATCTTGAAATGATTATTCAGTTTGGGTTCGTCACTTTATTTGTGGCCTCTTTCCCACTGGCTCCCCTG
  CTGGCTCTGGTGAACAATATTTTGGAAATAAGAGTGGACGCGTGGAAGCTCACAACCCAGTTTAGGCGC
  ATGGTGCCCGAAAAAGCCCAGGATATCGGCGCGTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCC
  GTGGTGACCAACGCCATGATCATTGCTTTCACATCAGACATGATCCCCCGCCTGGTGTATTATTGGTCT
  TTCTCTATCCCTCCCTACGGGGACCACACTTATTACACCATGGACGGCTACATCAACAACACTCTCTCT
  GTCTTCAACATCACGGACTTCAAGAACACAGACAAAGAAAACCCATACATTGGGCTTGGTAACTATACC
  TTGTGCAGGTACCGTGACTTCCGAAACCCACCTGGGCACCCACAAGAGTATAAACACAACATCTACTAT
  TGGCACGTGATTGCCGCCAAGCTGGCCTTCATCATTGTCATGGAGCACATCATCTACTCTGTGAAGTTC
  TTCATTTCCTACGCAATTCCAGATGTGTCCAAAATCACGAAAAGCAAGATCAAGAGGGAGAAATACCTC
  ACGCAGAAGTTGCTTCACGAGAGTCACCTCAAAGACCTGACCAAAAACATGGGGATCATCGCCGAGAGG
  ATAGGAGGAACGGTTGACAACAGCGTGCGGCCAAAACTCGAA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR211153
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MQMMTRKVLLNMELEEDDDEDGDIVLENFDQTIVCPTFGSLENQQDFRTPEFEEFNGKPDSLFFTDGQR
  RIDFILVYEDESKKENNKKGTNEKQKRKRQAYESNLICHGLQLEATRSVSDDKLVFVKVHAPWEVLCTY
  AEIMHIKLPLKPNDLKTRSPFGNLNWFTKVLRVNESVIKPEQEFFTAPFEKSRMNDFYILDRDSFFNPA
  TRSRIVYFILSRVKYQVMNNVNKFGINRLVSSGIYKAAFPLHDCRFNYESEDISCPSERYLLYREWAHP
  RSIYKKQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLDQDNCTWSKEVCDPDIGGQ
  ILMCPQCDRLCPFWRLNITCESSKKLCIFDSFGTLIFAVFMGVWVTLFLEFWKRRQAELEYEWDTVELQ
  QEEQARPEYEAQCNHVVINEITQEEERIPFTTCGKCIRVTLCASAVFFWILLIIASVIGIIVYRLSVFI
  VFSTTLPKNPNGTDPIQKYLTPQMATSITASIISFIIIMILNTIYEKVAIMITNFELPRTQTDYENSLT
  MKMFLFQFVNYYSSCFYIAFFKGKFVGYPGDPVYLLGKYRSEECDPGGCLLELTTQLTIIMGGKAIWNN
  IQEVLLPWVMNLIGRYKRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPL
  LALVNNILEIRVDAWKLTTQFRRMVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFTSDMIPRLVYYWS
  FSIPPYGDHTYYTMDGYINNTLSVFNITDFKNTDKENPYIGLGNYTLCRYRDFRNPPGHPQEYKHNIYY
  WHVIAAKLAFIIVMEHIIYSVKFFISYAIPDVSKITKSKIKREKYLTQKLLHESHLKDLTKNMGIIAER
  IGGTVDNSVRPKLE
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175344
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175344.4
• RefSeq Size: 5662 bp
• RefSeq ORF: 2736 bp
• Locus ID: 105722
• UniProt ID: Q6P9J9
• Cytogenetics: 15 E3-F1
• MW: 106.3 kDa
• Gene Summary: Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.[UniProtKB/Swiss-Prot Function]