Nrp1 (NM_008737) Mouse Tagged ORF Clone

CAT#: MR211197

Nrp1 (Myc-DDK-tagged) - Mouse neuropilin 1 (Nrp1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nrp1
• Synonyms: C530029I03; NP-1; NPN-1; Npn1; Nrp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211197 representing NM_008737
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGGGGCTGCCGTTGCTGTGCGCCACGCTCGCCCTTGCCCTCGCCCTGGCGGGCGCTTTCCGCA
  GCGACAAATGTGGCGGGACCATAAAAATCGAAAACCCAGGGTACCTCACATCTCCCGGTTACCCTCATTC
  TTACCATCCAAGTGAGAAGTGTGAATGGCTAATCCAAGCTCCGGAACCCTACCAGAGAATCATGATCAAC
  TTCAACCCACATTTCGATTTGGAGGACAGAGACTGCAAGTATGACTACGTGGAAGTAATCGATGGGGAGA
  ATGAAGGCGGCCGCCTGTGGGGGAAGTTCTGTGGGAAGATTGCACCTTCTCCTGTGGTGTCTTCAGGGCC
  CTTTCTCTTCATCAAATTTGTCTCTGACTATGAGACACATGGGGCAGGGTTTTCCATCCGCTATGAAATC
  TTCAAGAGAGGGCCCGAATGTTCTCAGAACTATACAGCACCTACTGGAGTGATAAAGTCCCCTGGGTTCC
  CTGAAAAATACCCCAACAGCTTGGAGTGCACCTACATCATCTTTGCACCAAAGATGTCTGAGATAATCCT
  GGAGTTTGAAAGTTTTGACCTGGAGCAAGACTCGAATCCTCCCGGAGGAATGTTCTGTCGCTATGACCGG
  CTGGAGATCTGGGATGGATTCCCTGAAGTTGGCCCTCACATTGGGCGTTATTGTGGGCAGAAAACTCCTG
  GCCGGATCCGCTCCTCTTCAGGCGTTCTATCCATGGTCTTTTACACTGACAGCGCAATAGCAAAAGAAGG
  TTTCTCAGCCAACTACAGTGTGCTACAGAGCAGCATCTCTGAAGATTTTAAGTGTATGGAGGCTCTGGGC
  ATGGAATCTGGAGAGATCCATTCTGATCAGATCACTGCATCTTCACAGTATGGTACCAACTGGTCTGTAG
  AGCGCTCCCGCCTGAACTACCCTGAAAATGGGTGGACTCCAGGAGAAGACTCCTACAAGGAGTGGATCCA
  GGTGGACTTGGGCCTCCTGCGATTCGTTACTGCTGTAGGGACACAGGGTGCCATTTCCAAGGAAACCAAG
  AAGAAATATTATGTCAAGACTTACAGAGTAGACATCAGCTCCAACGGAGAGGACTGGATCTCCCTGAAAG
  AGGGAAATAAAGCCATTATCTTTCAGGGAAACACCAACCCCACAGATGTTGTCTTAGGAGTTTTCTCCAA
  ACCACTGATAACTCGATTTGTCCGAATCAAACCTGTATCCTGGGAAACTGGTATATCTATGAGATTTGAA
  GTTTATGGCTGCAAGATAACAGATTATCCTTGCTCTGGAATGTTGGGCATGGTGTCTGGACTTATTTCAG
  ACTCCCAGATTACAGCATCCAATCAAGCCGACAGGAATTGGATGCCAGAAAACATCCGTCTGGTGACCAG
  TCGTACCGGCTGGGCACTGCCACCCTCACCCCACCCATACACCAATGAATGGCTCCAAGTGGACCTGGGA
  GATGAGAAGATAGTAAGAGGTGTCATCATTCAGGGTGGGAAGCACCGAGAAAACAAGGTGTTCATGAGGA
  AGTTCAAGATCGCCTATAGTAACAATGGCTCTGACTGGAAAACTATCATGGATGACAGCAAGCGCAAGGC
  TAAGTCGTTCGAAGGCAACAACAACTATGACACACCTGAGCTTCGGACGTTTTCACCTCTCTCCACAAGG
  TTCATCAGGATCTACCCTGAGAGAGCCACACACAGTGGGCTTGGGCTGAGGATGGAGCTACTGGGCTGTG
  AAGTGGAAGCACCTACAGCTGGACCAACCACACCCAATGGGAACCCAGTGGATGAGTGTGACGACGACCA
  GGCCAACTGCCACAGTGGCACAGGTGATGACTTCCAGCTCACAGGAGGCACCACTGTCCTGGCCACAGAG
  AAGCCAACCATTATAGACAGCACCATCCAATCAGAGTTCCCGACATACGGTTTTAACTGCGAGTTTGGCT
  GGGGCTCTCACAAGACATTCTGCCACTGGGAGCATGACAGCCATGCACAGCTCAGGTGGAGTGTGCTGAC
  CAGCAAGACAGGGCCGATTCAGGACCATACAGGAGATGGCAACTTCATCTATTCCCAAGCTGATGAAAAT
  CAGAAAGGCAAAGTAGCCCGCCTGGTGAGCCCTGTGGTCTATTCCCAGAGCTCTGCCCACTGTATGACCT
  TCTGGTATCACATGTCCGGCTCTCATGTGGGTACACTGAGGGTCAAACTACGCTACCAGAAGCCAGAGGA
  ATATGATCAACTGGTCTGGATGGTGGTTGGGCACCAAGGAGACCACTGGAAAGAAGGACGTGTCTTGCTG
  CACAAATCTCTGAAACTATATCAGGTTATTTTTGAAGGTGAAATCGGAAAAGGAAACCTTGGTGGAATTG
  CTGTGGATGATATCAGTATTAACAACCATATTTCTCAGGAAGACTGTGCAAAACCAACAGACCTAGATAA
  AAAGAACACAGAAATTAAAATTGATGAAACAGGGAGCACTCCAGGATATGAAGGAGAAGGGGAAGGTGAC
  AAGAACATCTCCAGGAAGCCAGGCAATGTGCTTAAGACCCTGGATCCCATCCTGATCACCATCATAGCCA
  TGAGTGCCCTGGGAGTACTCCTGGGTGCAGTCTGTGGAGTTGTGCTGTACTGTGCCTGTTGGCACAATGG
  GATGTCAGAAAGGAACCTATCTGCCCTGGAGAACTATAACTTTGAACTTGTGGATGGTGTAAAGTTGAAA
  AAAGATAAACTGAACCCACAGAGTAATTACTCAGAGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211197 representing NM_008737
  Red=Cloning site Green=Tags(s)
  
  MERGLPLLCATLALALALAGAFRSDKCGGTIKIENPGYLTSPGYPHSYHPSEKCEWLIQAPEPYQRIMIN
  FNPHFDLEDRDCKYDYVEVIDGENEGGRLWGKFCGKIAPSPVVSSGPFLFIKFVSDYETHGAGFSIRYEI
  FKRGPECSQNYTAPTGVIKSPGFPEKYPNSLECTYIIFAPKMSEIILEFESFDLEQDSNPPGGMFCRYDR
  LEIWDGFPEVGPHIGRYCGQKTPGRIRSSSGVLSMVFYTDSAIAKEGFSANYSVLQSSISEDFKCMEALG
  MESGEIHSDQITASSQYGTNWSVERSRLNYPENGWTPGEDSYKEWIQVDLGLLRFVTAVGTQGAISKETK
  KKYYVKTYRVDISSNGEDWISLKEGNKAIIFQGNTNPTDVVLGVFSKPLITRFVRIKPVSWETGISMRFE
  VYGCKITDYPCSGMLGMVSGLISDSQITASNQADRNWMPENIRLVTSRTGWALPPSPHPYTNEWLQVDLG
  DEKIVRGVIIQGGKHRENKVFMRKFKIAYSNNGSDWKTIMDDSKRKAKSFEGNNNYDTPELRTFSPLSTR
  FIRIYPERATHSGLGLRMELLGCEVEAPTAGPTTPNGNPVDECDDDQANCHSGTGDDFQLTGGTTVLATE
  KPTIIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDSHAQLRWSVLTSKTGPIQDHTGDGNFIYSQADEN
  QKGKVARLVSPVVYSQSSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMVVGHQGDHWKEGRVLL
  HKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPTDLDKKNTEIKIDETGSTPGYEGEGEGD
  KNISRKPGNVLKTLDPILITIIAMSALGVLLGAVCGVVLYCACWHNGMSERNLSALENYNFELVDGVKLK
  KDKLNPQSNYSEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008737
• ORF Size: 2769 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008737.1, NM_008737.2, NP_032763.1, NP_032763.2
• RefSeq Size: 5921 bp
• RefSeq ORF: 2772 bp
• Locus ID: 18186
• MW: 103.5 kDa