Gaa (NM_001159324) Mouse Tagged ORF Clone

CAT#: MR211293

Gaa (Myc-DDK-tagged) - Mouse glucosidase, alpha, acid (Gaa), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gaa
• Synonyms: E430018M07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211293 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATACGGAAGCCCCTCTGTTCGAACTCCGTGGTTGGGGCCTGCACCCTTATCTCTCTGACTACAG
  CGGTCATCCTGGGTCATCTCATGCTTCGGGAGTTAATGCTGCTTCCCCAAGACCTTCATGAGTCCTCTTC
  AGGACTGTGGAAGACGTACCGACCTCACCACCAGGAAGGTTACAAGCCAGGGCCTCTGCACATCCAGGAG
  CAGACTGAACAGCCCAAAGAAGCACCCACACAGTGTGATGTGCCCCCCAGCAGCCGCTTTGACTGTGCCC
  CCGACAAAGGCATCTCACAGGAGCAATGCGAGGCCCGCGGCTGCTGCTATGTCCCAGCAGGGCAGGTGCT
  GAAGGAGCCGCAGATAGGGCAGCCCTGGTGTTTCTTCCCTCCCAGCTACCCAAGCTACCGTCTAGAGAAC
  CTGAGCTCTACAGAGTCGGGGTACACAGCCACCCTGACCCGTACCAGCCCGACCTTCTTCCCAAAGGATG
  TGCTGACCTTACAGCTGGAGGTGCTGATGGAGACAGACAGCCGCCTCCACTTCAAGATCAAAGATCCTGC
  TAGTAAGCGCTACGAAGTGCCCCTGGAGACCCCACGTGTGCTGAGCCAGGCACCATCCCCACTTTACAGC
  GTGGAATTCTCAGAGGAACCCTTTGGAGTGATCGTTCGTAGGAAGCTTGGTGGCCGAGTGTTGCTGAACA
  CAACCGTGGCCCCCCTGTTCTTCGCTGACCAGTTCCTGCAGCTGTCCACTTCCCTGCCCTCCCAGCACAT
  CACAGGCCTGGGGGAACACCTCAGCCCACTCATGCTCAGCACCGACTGGGCTCGTATCACCCTCTGGAAC
  CGGGACACACCACCCTCGCAAGGTACCAACCTCTACGGGTCACATCCTTTCTACCTGGCACTGGAGGACG
  GTGGCTTGGCTCACGGTGTCTTCTTGCTAAACAGCAATGCCATGGATGTCATCCTGCAACCCAGCCCAGC
  CCTAACCTGGAGGTCAACGGGCGGGATCCTGGATGTGTATGTGTTCCTAGGCCCAGAGCCCAAGAGCGTT
  GTGCAACAATACCTGGATGTTGTGGGATACCCCTTCATGCCTCCATACTGGGGCCTCGGCTTCCACCTCT
  GCCGCTGGGGCTACTCCTCGACCGCCATTGTCCGCCAGGTAGTGGAGAACATGACCAGGACACACTTCCC
  GCTGGACGTGCAATGGAATGACCTGGACTACATGGACGCCCGAAGAGACTTCACCTTCAACCAGGACAGC
  TTTGCCGACTTCCCAGACATGGTGCGGGAGCTGCACCAGGATGGCCGGCGCTACATGATGATCGTGGACC
  CTGCCATCAGCAGCGCAGGCCCTGCTGGGAGTTACAGGCCCTACGACGAGGGTCTGCGGAGGGGCGTGTT
  CATCACCAACGAGACTGGGCAGCCGCTGATTGGGAAGGTTTGGCCTGGAACCACCGCCTTCCCTGATTTC
  ACCAACCCTGAGACCCTTGACTGGTGGCAGGACATGGTGTCTGAGTTCCACGCCCAGGTGCCCTTCGATG
  GCATGTGGCTCGACATGAACGAACCGTCCAACTTCGTTAGAGGCTCTCAGCAGGGCTGCCCCAACAATGA
  ACTGGAGAACCCCCCCTATGTGCCCGGGGTGGTTGGCGGGATCTTGCAGGCAGCCACCATCTGTGCCTCC
  AGCCACCAATTCCTCTCCACACACTACAACCTCCACAACCTGTACGGCCTCACTGAAGCTATCGCCTCCA
  GCAGGGCCCTGGTCAAGACTCGGGGAACACGACCCTTTGTGATCTCCCGCTCAACCTTCTCGGGCCACGG
  CCGGTACGCTGGTCACTGGACAGGGGATGTGCGGAGCTCTTGGGAGCATCTTGCATACTCTGTGCCAGAC
  ATCCTGCAGTTCAACCTGCTGGGCGTGCCCCTGGTCGGGGCGGACATCTGCGGCTTCATAGGAGACACGT
  CAGAAGAGCTGTGTGTGCGCTGGACCCAGTTGGGGGCCTTCTACCCCTTCATGCGGAACCACAATGACCT
  GAATAGCGTGCCTCAGGAGCCGTACAGGTTCAGCGAGACGGCGCAGCAGGCCATGAGGAAGGCCTTCGCC
  TTACGCTATGCCCTTCTGCCCTACCTGTACACTCTCTTCCACCGCGCCCACGTCAGAGGAGACACGGTGG
  CCCGGCCCCTCTTCCTGGAGTTCCCTGAGGATCCCAGCACCTGGTCTGTGGACCGCCAGCTCTTGTGGGG
  GCCGGCCCTGCTCATCACACCTGTGCTTGAGCCTGGGAAAACTGAAGTGACGGGCTACTTCCCCAAGGGC
  ACGTGGTACAACATGCAGATGGTGTCAGTGGATTCCCTCGGTACTCTCCCTTCTCCATCATCGGCTTCAT
  CCTTCAGATCTGCTGTCCAGAGCAAGGGGCAGTGGCTGACACTGGAAGCCCCACTGGATACCATCAACGT
  GCACCTGAGGGAGGGGTACATCATACCGCTGCAGGGTCCCAGCCTCACAACCACGGAGTCCCGAAAGCAG
  CCCATGGCTCTGGCTGTGGCATTAACAGCAAGCGGCGAGGCCGATGGGGAGCTGTTCTGGGACGACGGGG
  AGAGCCTCGCGGTTCTGGAGCGTGGGGCCTACACACTGGTCACCTTCTCAGCCAAGAACAATACCATTGT
  GAACAAGTTAGTGCGTGTGACCAAGGAGGGAGCTGAGCTACAACTGAGGGAGGTGACCGTCTTGGGAGTG
  GCCACAGCTCCTACCCAGGTCCTTTCCAACGGCATCCCTGTCTCCAATTTCACCTACAGCCCTGACAACA
  AGAGCCTGGCCATCCCTGTCTCACTGCTGATGGGAGAGCTGTTTCAAATCAGCTGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211293 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNIRKPLCSNSVVGACTLISLTTAVILGHLMLRELMLLPQDLHESSSGLWKTYRPHHQEGYKPGPLHIQE
  QTEQPKEAPTQCDVPPSSRFDCAPDKGISQEQCEARGCCYVPAGQVLKEPQIGQPWCFFPPSYPSYRLEN
  LSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFKIKDPASKRYEVPLETPRVLSQAPSPLYS
  VEFSEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLMLSTDWARITLWN
  RDTPPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGPEPKSV
  VQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDS
  FADFPDMVRELHQDGRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGTTAFPDF
  TNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCPNNELENPPYVPGVVGGILQAATICAS
  SHQFLSTHYNLHNLYGLTEAIASSRALVKTRGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPD
  ILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFA
  LRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTEVTGYFPKG
  TWYNMQMVSVDSLGTLPSPSSASSFRSAVQSKGQWLTLEAPLDTINVHLREGYIIPLQGPSLTTTESRKQ
  PMALAVALTASGEADGELFWDDGESLAVLERGAYTLVTFSAKNNTIVNKLVRVTKEGAELQLREVTVLGV
  ATAPTQVLSNGIPVSNFTYSPDNKSLAIPVSLLMGELFQISWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159324
• ORF Size: 2859 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159324.1, NM_001159324.2, NP_001152796.1
• RefSeq Size: 3448
• RefSeq ORF: 2862
• Locus ID: 14387
• MW: 106.2 kDa
• Gene Summary: This gene encodes a lysosomal acid glucosidase that is involved in the degradation of glycogen. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that cleaves alpha-1-4 and alpha-1-6 glycosidic bonds of glycogen, maltose and intermediate oligosaccharides within the lysosome. Mice lacking the encoded protein exhibit symptoms similar to human Pompe syndrome such as accumulation of glycogen in cardiac and skeletal muscle lysosomes resulting in reduced mobility and strength. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]