Fgd1 (NM_008001) Mouse Tagged ORF Clone

CAT#: MR211315

Fgd1 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgd1
• Synonyms: ZFYVE3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211315 representing NM_008001
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGGCCACCGAGTCCCGGGGGGCCCTGGGCCTTCGGATCCCGAACGCTCAGCGGCAAATACCCCCG
  GTGCCGCTCCGCTGGCCTGTGCCGACTCGGACCCTGGAGCCTTGGAACCCGGGCTGCCGGTGAGCAGGGG
  CTCAGGCACGGCTCTTGGAGGCCCTTTGGATCCTCAGTTTGTTGGACCCTCGGATGCCAGCCTGGGAGCG
  CCTCCAAGCTCCCGGGTCTTACCCTGCGGCCCTAGTCCTCAGCACCACCGGGCCCTGCGCTTCTCCTACC
  ACCTGGAGGGCTCGCAGCCTCGGCCTGGACTGCACCAGGGAAACCGGATCCTGGTTAAAAGTTTGTCCCT
  TGACCCTGGCCAGAGCCTTGAGCCTCATCCAGAAGGTCCCCAACGGCTTCGCTCAGATCCAGGCCCTCCC
  ACTGAAATCCCTGGCCCACGTCCTTCACCACTGAAGCGGGCACCGGGCCCAAAGCCACAGGTTCCCCCAA
  AGCCCAGCTACCTGCAGATGCCCCGGGTGCTTCCTCCTCCTGAGCCCATTCCTCCACCACCATCACGCCC
  TCTGCCTGCAGACCCTAGAGTGGCCAAGGGACTCGTCCCCAGAGCTGAGGCCAGCACTAGTTCTGCAGCA
  GTATCATCATTGATTGAGAAGTTTGAAAGAGAGCCTGTGATTGTTGCCTCGGATAGGCCAGCCCCTGGCC
  CCTGCCCAGTTCCCCCAGAGCCAGCCATGTTGCCACAGCCACCCCCGCAGCCAACAGGGTCCCAGCTCCC
  TGAGGGTGAAGCTTCTCGCTGCCTGTTCCTGCTGGCTCCTGGGCCCCGGGATGGTGAGAAGGTGCCTAAC
  CGGGACAGCGGCATTGACAGCATCAGCTCGCCATCCAACAGTGAAGAGACCTGCTTCGTCAGTGATGATG
  GGCCCCCCATCCACAGCCTCTGTCCTGGGCCCCCCGCCCTGGCTAGTATGCCAGTAGCTTTGGCCGACCC
  CCACCGGCCTGGCTCCCAGGAGGTTGACAGTGACCTGGAAGAGGAAGAGGAAGAAGAAGAGGAGGAAAAG
  GAAAGAGAAATTCCAGTGCCCCCGATGGAGAGACAGGAGTCTGTGGAGCTGACTGTGCAGCAGAAGGTGT
  TCCATATTGCCAATGAGCTCCTGCAAACTGAGAAGGCCTATGTTTCCAGGCTCCATCTCCTGGATCAGGT
  ATTCTGTGCCCGGCTGCTGGAAGAAGCTCGGAACCGCAGTTCCTTCCCCGCAGATGTTGTCCACGGCATC
  TTCTCTAACATCTGCTCCATCTATTGCTTCCACCAGCAGTTCCTGCTGCCTGAGCTAGAGAAGCGCATGG
  AGGAATGGGACCGCTACCCACGCATTGGTGACATTCTTCAGAAGTTGGCACCCTTCCTCAAGATGTATGG
  CGAGTATGTGAAAAACTTCGACCGGGCGGTAGAGCTGGTCAACACATGGACAGAGCGGTCTACCCAGTTT
  AAAGTCATCATCCATGAAGTACAGAAGGAGGAAGCCTGTGGCAACCTGACCTTGCAGCACCACATGCTGG
  AGCCCGTGCAGCGTATCCCCCGCTATGAACTTCTTCTCAAGGACTATCTGTTAAAGCTGCCTCATGGCTC
  CCCGGACAGCAAGGATGCCCAGAAGTCTTTGGAGCTGATAGCCACAGCAGCAGAACACTCTAATGCTGCC
  ATCCGGAAAATGGAACGAATGCACAAGCTGCTGAAGGTGTATGAGCTGCTAGGGGGTGAGGAGGACATTG
  TCAGTCCCACCAAAGAGCTCATAAAGGAAGGCCATATCCTTAAGCTGTCAGCAAAGAATGGGACCACTCA
  AGATCGATACCTCATATTATTCAACGACCGCCTCCTTTACTGCGTGCCCAGGCTGCGGCTCCTTGGTCAG
  AAGTTTAGCGTGCGGGCACGCATTGATGTAGATGGCATGGAGCTAAAGGAGAGCTCCAACCTCAACATGC
  CTCGGACCTTCCTGGTGTCAGGAAAGCAGCGCTCTCTCGAACTGCAGGCCAGGACTGAGGAGGAGAAGAA
  AGACTGGGTCCAGGCCATCAATTCCACCCTCCTGAAGCATGAACAGACCCTGGAGACTTTCAAACTGTTG
  AACTCAACAAACAGGGATGATGAAGATACTCCCCCTAACTCTCCAAACGTGGATCTTGGGAAGAGGGCAC
  CTACGCCTATCCGGGAAAAGGAAGTCACCATGTGCATGCGCTGCCAGGAGCCCTTCAATTCCATCACCAA
  ACGCAGGCACCACTGCAAGGCCTGTGGGCATGTGGTTTGTGGGAAATGCTCTGAGTTCCGAGCCCGACTC
  ATCTATGACAACAATCGTTCCAACCGTGTGTGCACTGATTGCTATGTGGCCTTGCATGGAGCACCTGGGA
  GCAGTCCAGCCTGTAGCCAGCATACACCCCAGCGCAGGAGGTCCATCCTAGAGAAACAGGCCTCGGTCGC
  TGCTGAGAACAGCGTCATCTGCAGCTTCCTGCACTACATGGAGAAGGGTGGGAAAGGATGGCACAAGGCA
  TGGTTTGTGGTTCCTGAGAATGAACCCTTGGTGCTGTACATCTATGGAGCCCCTCAGGATGTGAAAGCCC
  AGCGCAGCCTGCCCCTCATTGGCTTTGAGGTGGGGCCTCCTGAGGCAGGAGAGCGGCCAGACAGGAGGCA
  TGTCTTCAAGATCACACAAAGCCACCTAAGCTGGTACTTCAGCCCAGAGACAGAAGAACTCCAGCGGCGC
  TGGATGGCTGTTCTCGGCAGGGCGGGCCGTGGGGACACATTCTGCCCAGGGCCCACACTGTCTGAGGACA
  AGGAGATGGAGGAGACACCAGTGGCAGCCTCAGGAGCCACTGCTGAACCTCCTGAAGCCTCCCAGACCCG
  AGACAAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211315 representing NM_008001
  Red=Cloning site Green=Tags(s)
  
  MHGHRVPGGPGPSDPERSAANTPGAAPLACADSDPGALEPGLPVSRGSGTALGGPLDPQFVGPSDASLGA
  PPSSRVLPCGPSPQHHRALRFSYHLEGSQPRPGLHQGNRILVKSLSLDPGQSLEPHPEGPQRLRSDPGPP
  TEIPGPRPSPLKRAPGPKPQVPPKPSYLQMPRVLPPPEPIPPPPSRPLPADPRVAKGLVPRAEASTSSAA
  VSSLIEKFEREPVIVASDRPAPGPCPVPPEPAMLPQPPPQPTGSQLPEGEASRCLFLLAPGPRDGEKVPN
  RDSGIDSISSPSNSEETCFVSDDGPPIHSLCPGPPALASMPVALADPHRPGSQEVDSDLEEEEEEEEEEK
  EREIPVPPMERQESVELTVQQKVFHIANELLQTEKAYVSRLHLLDQVFCARLLEEARNRSSFPADVVHGI
  FSNICSIYCFHQQFLLPELEKRMEEWDRYPRIGDILQKLAPFLKMYGEYVKNFDRAVELVNTWTERSTQF
  KVIIHEVQKEEACGNLTLQHHMLEPVQRIPRYELLLKDYLLKLPHGSPDSKDAQKSLELIATAAEHSNAA
  IRKMERMHKLLKVYELLGGEEDIVSPTKELIKEGHILKLSAKNGTTQDRYLILFNDRLLYCVPRLRLLGQ
  KFSVRARIDVDGMELKESSNLNMPRTFLVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKLL
  NSTNRDDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRARL
  IYDNNRSNRVCTDCYVALHGAPGSSPACSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHKA
  WFVVPENEPLVLYIYGAPQDVKAQRSLPLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQRR
  WMAVLGRAGRGDTFCPGPTLSEDKEMEETPVAASGATAEPPEASQTRDKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008001
• ORF Size: 2880 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008001.1, NM_008001.2, NM_008001.3, NM_008001.4, NP_032027.2
• RefSeq Size: 3516
• RefSeq ORF: 2883
• Locus ID: 14163
• MW: 106.8 kDa
• Gene Summary: This gene encodes a member of family of Rho-specific guanine nucleotide exchange factors. Rho-specific guanine nucleotide exchange factors catalyze the exchange of GDP for GTP and activate small GTPases, which function as molecular switches in signaling. This protein specifically binds cell division cycle 42, a Rho (Ras homology) GTPase. Investigations in mouse suggest that this protein is important for skeletal mineralization and for regulating the actin cytoskeleton. In humans, mutations in this gene are associated with faciogenital dysplasia, also known as Aarskog-Scott syndrome. [provided by RefSeq, Mar 2014]