Trim37 (NM_197987) Mouse Tagged ORF Clone

CAT#: MR211317

Trim37 (Myc-DDK-tagged) - Mouse tripartite motif-containing 37 (Trim37)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim37
• Synonyms: 1110032A10Rik; 2810004E07Rik; AI848587; AU043018; MUL; TEF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211317 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGCAGAGTGTGGAGAGCATTGCTGAGGTTTTCCGATGTTTCATTTGTATGGAGAAATTGCGGG
  ATGCTCGACTGTGTCCTCATTGCTCCAAGCTCTGTTGTTTCAGCTGTATTAGGCGCTGGCTGACAGAGCA
  AAGAGCTCAGTGTCCTCACTGTCGTGCTCCACTCCAGCTACGGGAACTGGTGAACTGTCGTTGGGCGGAA
  GAAGTGACACAGCAGCTTGACACTCTCCAGCTGTGTAGTCTCACCAAACATGAAGAAAATGAGAAAGACA
  AATGTGAAAATCACCATGAAAAACTTAGTGTGTTTTGCTGGACTTGTAAGAAGTGTATCTGCCATCAGTG
  TGCACTTTGGGGAGGAATGCATGGTGGACACACGTTTAAACCTTTGGCAGAAATTTATGAACAACATGTC
  ACTAAAGTGAATGAAGAGGTAGCCAAACTTCGTCGACGTCTCATGGAGCTGATCAGCTTAGTTCAAGAAG
  TGGAAAGAAATGTAGAAGCTGTAAGAAATGCAAAGGACGAGCGTGTTCGGGAAATTAGGAATGCGGTAGA
  GATGATGATTGCACGACTAGATACACAGCTGAAGAATAAGCTCATAACTCTCATGGGTCAGAAGACATCT
  CTTACTCAAGAAACGGAGCTGTTGGAATCTTTACTTCAGGAGGTAGAACATCAGTTGCGGTCTTGCAGTA
  AGAGCGAGCTGATCTCCAAGAGCTCAGAGATCCTAATGATGTTCCAGCAAGTTCACCGAAAGCCCATGGC
  ATCCTTTGTCACCACACCTGTCCCACCAGACTTTACCAGTGAATTGGTACCATCCTATGATTCAGCTACT
  TTTGTTTTAGAGAACTTCAGCACTTTGCGGCAGAGAGCAGATCCTGTTTACAGTCCGCCTCTTCAAGTTT
  CAGGACTTTGTTGGAGGTTAAAAGTTTACCCAGATGGAAATGGAGTTGTACGTGGCTATTATTTATCTGT
  ATTTTTGGAACTATCGGCTGGCTTGCCTGAAACTTCCAAGTATGAATATCGTGTAGAAATGGTTCACCAG
  TCGTGCAATGATCCTACAAAAAATATCATTCGAGAATTTGCATCTGACTTTGAAGTTGGAGAGTGCTGGG
  GCTACAATAGGTTTTTTCGTTTGGATTTACTTGCAAACGAAGGATACTTGAACCGACAAAATGATACAGT
  GATTTTAAGGTTTCAGGTGCGCTCACCAACATTTTTTCAAAAATGCCGGGATCAGCACTGGTATATTACT
  CAGTTGGAAGCTGCACAGACTGGTTATATCCAACAAATAAACAATCTTAAAGAGAGACTGACTATTGAGC
  TGTCCCGAACTCAGAAATCCAGAGATCTGTCACCACCAGATAATCATCTTAGCCCTCAAAATGATGATTC
  TCCTGAGACACGAACTAAGAAAGCTGGGTCATGCTCTGACATGCTTCTGGAAGGCGGTCCTACTTGCGCT
  TCTGTACGAGAGACCAAGGAAGATGAAGATGAGGAGGAGAAGATTCAGAATGAAGACTATCATCATGAGC
  TCTCGGATGGAGATCTGGACCTGGATCTTGTTGGAGAAGATGAAGTGAATCACCTTGATGGCAGCAGCTC
  TTCTGCTAGTTCTACAGCAACAAGCAACACAGAAGAAAACGATATTGATGAGGAGACCATGTCTGGGGAA
  AATGATGTAGAATATAACAGTATGGAGCTGGAAGAGGGAGAGCTCATGGAAGATGCAGCTGCTGCAGGTC
  CTCCAGGTAGTAGCCACAGCTATGTGGGTGCCAGTAGCAGAATGTCAAGAAGAACACATTTATGCTCTGC
  AGCTACCAGTAGCTTACTAGACATTGATCCTTTAATCTTAATACATTTATTGGATCTTAAGGACCGGAGC
  AGTATGGAAAACCTGTGGGGTTTACAGCCTCGCCCGTCTGCTTCACTGTTGCAGCCCACAGCATCATATT
  CTCGAAAAGATAAAGATCAAAGGAAGCAGCAAGCGATGTGGCGTGTGCCCTCTGACCTAAAGATGCTGAA
  AAGGCTCAAAACACAGATGGCTGAAGTCCGGTGTATGAAGACTGATGTGAAGACTACGCTGTCAGATATA
  AAGGGCAGCAGTGTTGCTTCCACAGACATGCAGACAAACCTATTCTGTGCTGACCAGGCAGCTCTGACTA
  CCTGTGGACCCGAAAACTCTGGTAGATTACAGGATTTGGGAATGGAGCTTCTAGCAAAGTCATCAGTGGC
  TGGCTGTTACATACGAAACCCCACAAATAAGAAGAATTCCCCCAAGTCAGCTCGGGCCATAGCAGGCAGT
  CTGTCACTCCGAAGAGCTGTGGACTCTGGAGAAAATAGCCGTTCAAAGGGAGACTGTCAGGTTCTTGCTG
  AAGGCTCCTCGGGAAGCTCTCAGTCTGGGAGCAGACACAGCTCCCCCCGGGCTCTGACACACGGCATCAT
  TGGGGATCTTCTGCCCAAAAGTGAAGACCGACAGTGCAAAGCCTTGGATTCTGACGCTGTGGTTGTTGCA
  GTTTTCAATGGTTTACCTACTGTTGAGAAGAGAAGGAAAATGGTCACCTTGGGGACTAATGCAAAAGGAG
  GTCGTCTGGAAGGAATGCAGATGGCAGATTTGGAAAGTCATTCTGAAGCTGGGGAGGTACAGCCCACACT
  ACCTGAAGGAGCCTCAGCCGCCCCTGAGGAAGGAATGAGTAGTGACAGCGACATTGAATGTGACACTGAG
  AACGAGGAGCAGGAAGAACACACCAGCATGGGCGCGTTCAACGATCCGTTCCTGGCTCAGCCCCCCGATG
  AAGATTCACATTCCAGTTTTCCTGATGGTGAACAAATAGACCCTGAAAATCTCCACTTCAACCCTGATGA
  AGGAGGTGGAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211317 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDEQSVESIAEVFRCFICMEKLRDARLCPHCSKLCCFSCIRRWLTEQRAQCPHCRAPLQLRELVNCRWAE
  EVTQQLDTLQLCSLTKHEENEKDKCENHHEKLSVFCWTCKKCICHQCALWGGMHGGHTFKPLAEIYEQHV
  TKVNEEVAKLRRRLMELISLVQEVERNVEAVRNAKDERVREIRNAVEMMIARLDTQLKNKLITLMGQKTS
  LTQETELLESLLQEVEHQLRSCSKSELISKSSEILMMFQQVHRKPMASFVTTPVPPDFTSELVPSYDSAT
  FVLENFSTLRQRADPVYSPPLQVSGLCWRLKVYPDGNGVVRGYYLSVFLELSAGLPETSKYEYRVEMVHQ
  SCNDPTKNIIREFASDFEVGECWGYNRFFRLDLLANEGYLNRQNDTVILRFQVRSPTFFQKCRDQHWYIT
  QLEAAQTGYIQQINNLKERLTIELSRTQKSRDLSPPDNHLSPQNDDSPETRTKKAGSCSDMLLEGGPTCA
  SVRETKEDEDEEEKIQNEDYHHELSDGDLDLDLVGEDEVNHLDGSSSSASSTATSNTEENDIDEETMSGE
  NDVEYNSMELEEGELMEDAAAAGPPGSSHSYVGASSRMSRRTHLCSAATSSLLDIDPLILIHLLDLKDRS
  SMENLWGLQPRPSASLLQPTASYSRKDKDQRKQQAMWRVPSDLKMLKRLKTQMAEVRCMKTDVKTTLSDI
  KGSSVASTDMQTNLFCADQAALTTCGPENSGRLQDLGMELLAKSSVAGCYIRNPTNKKNSPKSARAIAGS
  LSLRRAVDSGENSRSKGDCQVLAEGSSGSSQSGSRHSSPRALTHGIIGDLLPKSEDRQCKALDSDAVVVA
  VFNGLPTVEKRRKMVTLGTNAKGGRLEGMQMADLESHSEAGEVQPTLPEGASAAPEEGMSSDSDIECDTE
  NEEQEEHTSMGAFNDPFLAQPPDEDSHSSFPDGEQIDPENLHFNPDEGGGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_197987
• ORF Size: 2886 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_197987.1, NM_197987.2, NP_932104.1
• RefSeq Size: 5637 bp
• RefSeq ORF: 2886 bp
• Locus ID: 68729
• MW: 107.7 kDa
• Gene Summary: The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which is typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. In mouse this protein is proposed to oligomerize through its coiled coil domain and has been reported to be expressed in neural crest-derived tissues as well as in tissues whose development is regulated by mesenchymal-epithelial interactions. In humans, mutations in this gene are associated with mulibrey (muscle-liver-brain-eye) nanism, an autosomal recessive disorder characterized by prenatal onset growth failure, cardiomyopathy and dysmorphic features. [provided by RefSeq, Jan 2013]