Atp2a1 (NM_007504) Mouse Tagged ORF Clone

CAT#: MR211405

Atp2a1 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 (Atp2a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp2a1
• Synonyms: SERCA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211405 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGCGCACTCCAAGTCCACAGAGGAATGTTTGTCCTATTTCGGGGTGAGCGAGACCACAGGCC
  TTACCCCAGACCAAGTTAAGCGACATCTGGAAAAATACGGCCCCAATGAGCTCCCTGCTGAGGAAGGCAA
  GTCTCTGTGGGAGTTGGTGGTAGAGCAGTTCGAAGACCTGCTTGTGCGGATTCTTCTCCTGGCTGCCTGC
  ATTTCCTTTGTGCTGGCCTGGTTTGAGGAAGGCGAAGAAACCGTCACTGCCTTTGTGGAACCTTTTGTCA
  TTCTCTTGATCCTCATTGCCAACGCCATTGTGGGGGTTTGGCAGGAACGGAATGCAGAGAACGCTATCGA
  AGCGCTGAAAGAATATGAACCCGAGATGGGAAAGGTCTATCGGGCTGACCGAAAGTCAGTGCAAAGGATC
  AAGGCTCGGGACATCGTTCCTGGGGACATCGTGGAGGTGGCCGTTGGGGACAAAGTCCCTGCAGACATCC
  GCATCCTCTCCATCAAATCCACCACCCTCCGGGTCGACCAGTCCATCCTGACAGGAGAGTCTGTGTCTGT
  CATCAAGCACACAGACCCTGTCCCTGACCCCCGAGCTGTCAACCAGGACAAGAAGAACATGCTCTTCTCA
  GGTACCAACATCGCAGCCGGCAAGGCTGTAGGCATCGTGGCCACCACTGGTGTGAGTACTGAGATTGGCA
  AGATCCGAGACCAGATGGCTGCCACCGAGCAGGACAAGACTCCACTGCAGCAGAAGCTTGACGAGTTTGG
  GGAGCAGCTGTCCAAGGTCATCTCTCTCATCTGTGTGGCTGTCTGGCTTATCAACATTGGCCACTTCAAC
  GATCCTGTCCATGGGGGCTCCTGGTTCCGGGGTGCCATTTACTACTTCAAGATTGCTGTGGCCTTGGCTG
  TGGCGGCCATCCCAGAAGGGCTTCCTGCTGTCATCACCACCTGCTTGGCTTTGGGTACCCGCCGGATGGC
  AAAGAAGAACGCCATCGTGAGGAGTCTGCCCTCTGTGGAGACCCTGGGCTGTACCTCTGTCATCTGTTCT
  GACAAAACAGGGACCCTCACCACCAACCAGATGTCAGTTTGCAAGATGTTCATCATTGACAAGGTGGATG
  GGGATGTCTGCTCACTGAACGAGTTCTCCATCACTGGCTCTACTTATGCTCCTGAGGGAGAGGTCTTGAA
  GAATGATAAGCCAGTCCGAGCAGGGCAGTATGATGGGCTGGTGGAGCTCGCTACCATCTGTGCCCTCTGC
  AATGATTCCTCCTTGGACTTTAATGAGACCAAAGGTGTCTATGAGAAGGTGGGCGAGGCCACGGAGACTG
  CTCTCACCACCTTGGTGGAGAAGATGAACGTATTCAACACGGAAGTGAGAAGCCTCTCTAAAGTGGAGCG
  GGCCAACGCCTGCAATTCGGTGATCCGCCAACTCATGAAGAAGGAATTCACCCTGGAATTTTCCCGAGAC
  AGGAAGTCTATGTCTGTCTACTGTTCACCAGCTAAGTCCTCACGGGCTGCCGTGGGCAACAAGATGTTTG
  TCAAGGGTGCCCCCGAGGGGGTCATCGACCGCTGTAACTATGTGCGTGTTGGCACGACCCGTGTGCCATT
  GACAGGCCCCGTGAAGGAGAAGATCATGTCTGTGATCAAGGAGTGGGGCACTGGTCGGGACACACTGCGG
  TGCCTGGCGCTGGCCACCCGCGACACGCCCCCCAAACGCGAGGAGATGGTTCTGGATGATTCAGCCAAGT
  TCATGGAGTATGAGATGGACCTGACGTTTGTTGGTGTTGTGGGCATGTTGGATCCACCCCGCAAGGAGGT
  CACAGGCTCTATCCAGCTGTGTCGTGATGCTGGGATCCGAGTGATCATGATCACTGGGGACAACAAGGGT
  ACAGCCATTGCCATCTGCCGACGAATTGGCATCTTTTCGGAGAATGAGGAAGTGACAGACCGTGCCTACA
  CTGGCCGTGAGTTTGACGATCTGCCCCTGGCCGAGCAGCGGGAGGCCTGCAGACGCGCCTGCTGCTTTGC
  TCGTGTGGAGCCCTCACACAAGTCCAAGATTGTGGAGTACCTGCAGTCCTACGATGAGATCACAGCCATG
  ACAGGGGATGGTGTCAATGATGCCCCTGCTCTGAAGAAGGCCGAGATTGGCATTGCCATGGGATCTGGTA
  CTGCTGTGGCTAAGACGGCCTCTGAAATGGTACTGGCCGATGATAACTTCTCCACCATCGTGGCTGCCGT
  GGAGGAAGGCCGCGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCTCCAATGTGGGC
  GAGGTGGTCTGTATCTTCTTGACAGCAGCCTTGGGGCTGCCTGAGGCTCTGATCCCTGTGCAGCTGCTCT
  GGGTGAACTTGGTGACTGATGGGCTCCCGGCTACTGCCCTGGGATTCAACCCACCTGACCTGGACATCAT
  GGACCGCCCCCCCAGGAGTCCCAAGGAGCCTCTTATCAGTGGCTGGCTCTTTTTCCGCTACATGGCAATT
  GGGGGCTATGTGGGTGCAGCCACTGTAGGAGCAGCTGCCTGGTGGTTTTTGTATGCAGAGGACGGGCCTC
  ATGTCAGCTACCATCAGCTGACTCATTTCATGCAGTGCACTGAGCACAACCCTGAATTCGATGGCCTGGA
  CTGTGAGGTCTTTGAGGCCCCCGAGCCCATGACCATGGCCTTGTCTGTGTTGGTGACCATCGAGATGTGC
  AATGCTCTCAACAGCCTGTCTGAGAACCAGTCCCTACTGCGGATGCCACCCTGGGTGAACATCTGGCTGC
  TGGGTTCCATCTGCCTGTCCATGTCCCTCCACTTCCTCATCCTCTATGTCGACCCCCTGCCGATGATCTT
  CAAGCTCCGGGCCCTGGACTTTACCCAGTGGCTCATGGTCCTCAAGATCTCACTGCCAGTCATCGGGCTG
  GATGAGCTTCTCAAGTTCATTGCTCGGAACTATCTGGAGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211405 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEAAHSKSTEECLSYFGVSETTGLTPDQVKRHLEKYGPNELPAEEGKSLWELVVEQFEDLLVRILLLAAC
  ISFVLAWFEEGEETVTAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRI
  KARDIVPGDIVEVAVGDKVPADIRILSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFS
  GTNIAAGKAVGIVATTGVSTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFN
  DPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICS
  DKTGTLTTNQMSVCKMFIIDKVDGDVCSLNEFSITGSTYAPEGEVLKNDKPVRAGQYDGLVELATICALC
  NDSSLDFNETKGVYEKVGEATETALTTLVEKMNVFNTEVRSLSKVERANACNSVIRQLMKKEFTLEFSRD
  RKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMSVIKEWGTGRDTLR
  CLALATRDTPPKREEMVLDDSAKFMEYEMDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKG
  TAIAICRRIGIFSENEEVTDRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAM
  TGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVG
  EVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAI
  GGYVGAATVGAAAWWFLYAEDGPHVSYHQLTHFMQCTEHNPEFDGLDCEVFEAPEPMTMALSVLVTIEMC
  NALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDFTQWLMVLKISLPVIGL
  DELLKFIARNYLEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007504
• ORF Size: 2985 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007504.2, NP_031530.2
• RefSeq Size: 3486 bp
• RefSeq ORF: 2985 bp
• Locus ID: 11937
• MW: 109.4 kDa