Sall2 (NM_015772) Mouse Tagged ORF Clone

CAT#: MR211432

Sall2 (Myc-DDK-tagged) - Mouse sal-like 2 (Drosophila) (Sall2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sall2
• Synonyms: AI225809; AW559097; mKIAA0360; Msal-2; p150(Sal2); Sal-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211432 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGCGAAAGCAACGGAGACCCCAACAGTTAATCTCGGACTGTGAAGGTCCCAGCGCATCTGAGA
  ACGGTGATGCTAGCGAGGAAGACCACCCCCAAGTCTGTGCCAAATGCTGCGCACAATTCTCTGACCCGAC
  CGAATTCCTCGCTCACCAGAACTCATGTTGCACTGACCCACCGGTAATGGTGATAATTGGAGGCCAGGAG
  AATCCCAGCAACTCTTCAGCCTCCTCTGCGCCCCGACCAGAGGGCCACAGTAGGTCCCAGGTCATGGATA
  CAGAGCACAGCAATCCCCCAGATTCTGGGTCCTCTGGGGCCCCGGATCCCACTTGGGGGCCAGAGCGGAG
  GGGAGAGGAATCTTCTGGGCAATTCCTGGTCGCTGCCACAGGTACAGCGGCTGGGGGAGGTGGGGGCCTT
  ATCTTGGCCAGTCCCAAGCTGGGAGCAACCCCATTACCTCCAGAATCCACTCCTGCACCCCCTCCTCCCC
  CACCTCCCCCTCCCCCTCCAGGTGTAGGCAGTGGCCACTTGAACATTCCTCTGATCTTGGAAGAGTTGCG
  GGTGCTGCAGCAGCGCCAGATTCACCAGATGCAGATGACTGAACAAATCTGCCGCCAGGTGCTGCTACTT
  GGCTCCTTGGGGCAGACCGTGGGTGCCCCTGCCAGTCCCTCAGAGCTACCTGGGACAGGGGCTGCCTCTT
  CCACCAAGCCCCTACTGCCTCTCTTCAGTCCCATCAAGCCAGCGCAAACTGGCAAGACACTGGCATCTTC
  CTCTTCGTCATCCTCCTCCTCTGGAGCTGAACCGCCTAAGCAGGCTTTCTTCCACCTTTACCATCCACTG
  GGATCACAGCATCCCTTCTCTGTAGGAGGGGTTGGGCGGAGCCACAAACCCACCCCTGCCCCTTCCCCTG
  CGCTGCCAGGCAGTACGGATCAGCTGATTGCTTCACCTCATCTGGCATTCCCAGGCACCACCGGACTCCT
  GGCAGCTCAGTGTCTTGGGGCAGCAAGGGGCCTTGAGGCTGCTGCCTCCCCAGGGCTCCTGAAGCCAAAG
  AACGGAAGTGGTGAACTGGGCTATGGGGAAGTGATCAGTTCCTTGGAGAAACCCGGTGGAAGGCACAAAT
  GCCGCTTTTGTGCAAAAGTATTCGGCAGTGACAGCGCCCTGCAGATCCACCTTCGTTCCCACACTGGTGA
  GAGGCCCTATAAGTGCAACGTCTGTGGTAACCGTTTCACAACTCGGGGCAACCTCAAAGTACATTTTCAC
  CGGCATCGTGAGAAGTACCCACATGTGCAAATGAATCCACATCCAGTACCGGAGCACCTAGACTACGTCA
  TCACCAGCAGTGGGCTGCCTTACGGAATGTCTGTGCCACCAGAGAAAGCAGAAGAGGAGGCAGGCACACC
  AGGCGGAGGTGTTGAACGCAAACCCCTAGTGGCCTCCACCACAGCACTCAGTGCCACAGAGAGCCTGACA
  CTGCTCTCCACTGGCACAAGCACAGCAGTGGCTCCTGGGCTCCCTACTTTCAACAAGTTTGTGCTCATGA
  AGGCAGTGGAACCCAAGAGTAAAGCGGATGAGAACACGCCCCCAGGGAGTGAGGGCTCCGCCATCGCTGG
  AGTAGCAGACAGTGGCTCAGCAACCCGAATGCAGCTAAGTAAGCTGGTGACGTCACTACCGAGTTGGGCA
  CTGCTTACTAATCACTTGAAGTCAACTGGAAGTTTCCCCTTCCCTTATGTGCTAGAACCCTTGGGGGCTT
  CGCCTTCTGAGACCTCAAAGCTGCAGCAGCTAGTAGAAAAGATTGACCGCCAAGGAGCTGTGGCGGTGGC
  ATCTACTGCCTCGGGAGCTCCCACCACTTCTGCCCCTGCACCTTCCTCCTCCGCTTCTGGACCTAACCAG
  TGTGTGATCTGTCTTCGGGTCCTGAGCTGCCCTCGGGCTCTACGCCTGCATTATGGCCAACATGGAGGTG
  AGCGGCCCTTCAAGTGTAAAGTGTGTGGCCGAGCTTTCTCCACAAGGGGCAATTTGCGCGCACATTTCGT
  GGGTCACAAGACCAGTCCAGCTGCCCGGGCTCAGAACTCCTGCCCCATTTGTCAGAAGAAGTTCACTAAT
  GCTGTCACTCTGCAGCAACATGTTCGGATGCACCTGGGGGGCCAGATCCCCAATGGGGGTTCCGCACTTT
  CTGAAGGTGGGGGAGCTGCCCAGGAAAACAGCTCTGAGCAGTCTACAGCCTCTGGACCAGGGAGTTTCCC
  CCAGCCGCAGTCCCAGCAGCCATCTCCAGAAGAGGAGATGTCTGAGGAAGAGGAAGAGGATGAGGAAGAG
  GAGGAAGACGTGACAGATGAAGATTCCCTAGCAGGAAGAGGCTCTGAGAGTGGGGGAGAGAAGGCCATAT
  CAGTACGAGGTGACTCAGAAGAGGTATCTGGGGCAGAGGAAGAAGTGGCAACATCAGTAGCAGCACCCAC
  CACTGTGAAGGAGATGGACAGTAATGAGAAAGCCCCTCAACACACTCTGCCGCCACCTCCGCCACCACCC
  GACAACCTGGATCATCCCCAACCCATGGAGCAGGGAACCAGTGATGTTTCCGGAGCCATGGAGGAAGAAG
  CCAAACTGGAGGGAACCTCAAGCCCGATGGCAGCCCTCACCCAAGAAGGGGAGGGCACCAGCACCCCTTT
  GGTGGAAGAGCTGAACTTACCGGAAGCCATGAAGAAGGATCCAGGAGAGAGCAGCGGCAGGAAGGCCTGT
  GAAGTATGTGGCCAGAGCTTTCCTACCCAGACAGCTCTGGAGGAGCATCAGAAGACCCATCCCAAGGATG
  GGCCACTCTTCACTTGTGTCTTCTGCAGGCAGGGCTTCCTTGACCGTGCTACCCTCAAGAAGCACATGCT
  GTTGGCTCACCACCAGGTACCGCCCTTTGCACCCCATGGCCCTCAGAATATTGCTACTCTTTCCTTGGTC
  CCTGGCTGTTCCTCCTCCATCCCTTCTCCAGGGCTCTCCCCATTCCCTCGAAAAGATGACCCCACCATGC
  CA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211432 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRRKQRRPQQLISDCEGPSASENGDASEEDHPQVCAKCCAQFSDPTEFLAHQNSCCTDPPVMVIIGGQE
  NPSNSSASSAPRPEGHSRSQVMDTEHSNPPDSGSSGAPDPTWGPERRGEESSGQFLVAATGTAAGGGGGL
  ILASPKLGATPLPPESTPAPPPPPPPPPPPGVGSGHLNIPLILEELRVLQQRQIHQMQMTEQICRQVLLL
  GSLGQTVGAPASPSELPGTGAASSTKPLLPLFSPIKPAQTGKTLASSSSSSSSSGAEPPKQAFFHLYHPL
  GSQHPFSVGGVGRSHKPTPAPSPALPGSTDQLIASPHLAFPGTTGLLAAQCLGAARGLEAAASPGLLKPK
  NGSGELGYGEVISSLEKPGGRHKCRFCAKVFGSDSALQIHLRSHTGERPYKCNVCGNRFTTRGNLKVHFH
  RHREKYPHVQMNPHPVPEHLDYVITSSGLPYGMSVPPEKAEEEAGTPGGGVERKPLVASTTALSATESLT
  LLSTGTSTAVAPGLPTFNKFVLMKAVEPKSKADENTPPGSEGSAIAGVADSGSATRMQLSKLVTSLPSWA
  LLTNHLKSTGSFPFPYVLEPLGASPSETSKLQQLVEKIDRQGAVAVASTASGAPTTSAPAPSSSASGPNQ
  CVICLRVLSCPRALRLHYGQHGGERPFKCKVCGRAFSTRGNLRAHFVGHKTSPAARAQNSCPICQKKFTN
  AVTLQQHVRMHLGGQIPNGGSALSEGGGAAQENSSEQSTASGPGSFPQPQSQQPSPEEEMSEEEEEDEEE
  EEDVTDEDSLAGRGSESGGEKAISVRGDSEEVSGAEEEVATSVAAPTTVKEMDSNEKAPQHTLPPPPPPP
  DNLDHPQPMEQGTSDVSGAMEEEAKLEGTSSPMAALTQEGEGTSTPLVEELNLPEAMKKDPGESSGRKAC
  EVCGQSFPTQTALEEHQKTHPKDGPLFTCVFCRQGFLDRATLKKHMLLAHHQVPPFAPHGPQNIATLSLV
  PGCSSSIPSPGLSPFPRKDDPTMP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015772
• ORF Size: 3015 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015772.1, NM_015772.2, NM_015772.3, NP_056587.2
• RefSeq Size: 5058 bp
• RefSeq ORF: 3015 bp
• Locus ID: 50524
• MW: 104.9 kDa