Ccp110 (NM_182995) Mouse Tagged ORF Clone

CAT#: MR211433

Ccp110 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330503K22 gene (6330503K22Rik)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccp110
• Synonyms: 6330503K22Rik; AA415922; AI427129; AW557948; cep110; CP110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211433 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAATATGAGGAGTTCTGCGAGAAAGCACTTGGCCGAGCTCAGGAAGCATCGCTGTCCACAGGCA
  GCTTCCTGCCCGCTCAGGCAGAAAGTGTCTCACTCATCCGTTTCCATGGGGTGGCTGTACTTTCTCCACT
  GCTTACCATTGAGAAGAGAAAGAAAATTCAAGAGGAAAAGCAGAAAGCACTTGATGTGCAGTCAAGAAAG
  CAGGCCAACAGGAAGAAAGCCTTACTGACTCGTGTCCAGGAGATTCTTGAAAATGTTCAGGTTAGAAAAG
  CACCAAATGCCAGTGATTTTGATCAGTGGGCAACAGAAACCATTTACTCTAATCCAGAAGTCACAGACTT
  GAATGTTCCTGTTAGAGTTCCAAACAGCTTGCCGAGCCCCACCGAACACTGTACTTCAGTAAAGCTTGAA
  AAGATAACTGGACTTTTGCCTGTGAATAATGAGGACCAACAAACCCCTAAGCGGGTGGGGCTGCCTGGAG
  ACTCAGAGGTGTCTGGTTCTCTGAGGCAGTGTGAGAGTCCAGAAAGCAGGCAGGCAGAAGATGGAGCTGC
  TCTAAGGCTTTCTTCAGCAAGCCCACAGGAGACCATCATTTCTGATGTCTTAGGAAAGGAAGAACAGGAC
  CCATCATGTTTGGCTGAAGTCACTCCAGATCCCTACATAATGAGCCTTCAGAATCTAATGAAGAGGTCAA
  AGGAATACGTGGAAAGAGAACTGTCTAGCCGGAGTCTGAGAAATAGTTTAAAGAGAAGTGTTAATGAAAC
  GCATTCAGACAGAGAAAACGATGCTGCTAAGGCGAGCGACTGTGTAAAGGAGAAGGCCCCACCCATGCCC
  ATAGGCAGGCACTGTGGCTCTGCCATCCCTGACAAACCAAGCCTTAATAAGTCAAATGTTCTCCTCCAAG
  GTGCTTCACAAGCAAGCAGCATGGGCACGGCAGGCTTAGCTAGCTTTTCTAAGATAGACCTGCCTGCAGG
  AGCTGCTCCTCCCGCTGCTCCAGATGCCGGATCAGATTTTACAGTTATTCCTACCTTTGTTACTGAAAAT
  AAAGTTAAAAGTCTTAAAGGTCCATATGCCAAATTACCTAGTCCAGAGCCAAGTATGAGTCCTACGATGC
  ATCGGAGGCATTCTCGGTCAGCATCAGCATGTCAGATCCTTATAAACAACCCAGTAAATGCCTGTGAGCT
  GAGCCCAAAGGGGAAAGAAGAGGCAGTGGATAGAACCGCTCCAGCTGCTGCTGAGACAACAAATGAGTCT
  GAAACTGTGCCAAAATCACCGACTGATTTGACAGGGGTTTGTTCAAGTAATGTTTCTGCTACCAAAATCA
  CGTCTGAGAGCACAAGAGAGATGGTCGTGGGTAAGCCAAGTCAGAGGCAGCAGGCTTTAGGAGCTCACTT
  AGGCAATAACGTCACTGTTGAGCGGTCTGCTATGGAAGGTCCCTTCATAGCTGATGACAGGGGAGCCCAG
  AAGGTGGACGGTACTTGTATGGCTGTGCCCAAGTTGCATGAACTGCAGCCCAGCAGTCAGTGTGTGTCAA
  GTCAAACCTTGGAAGATGTGTGTGAACTCAAGTCAGCCAGTCTGCTAGCAAAGAACTCCTGCAACTTACA
  AATGGAACTGAATAAATCTTACGATGTGAAACACCCATCTCCCTTACTTACGCAAACCCAGACTTCCAGA
  CAGCAGATGGACACACCGCCGGTGTTCCGTGGAAACGAGCAGTTTGTAGATAACAGTTTTGAGAAAGTTA
  AGCGGAGACTTGATTTAGATGTTGATAGTTTACAAAAAGAAAATTGTCCTTATATTATAACAGCTGGAGT
  AGCTGAGCAGGAGCGGGACCGTCTGCTGGAAAGAAGATACCCCAAGGGATTCGTCCACATTAACAAGAAT
  AAGATGCTAGAAACTAGCCCCAAAGAAGGCCAGGAACTACTGAAAAGCAAGATGTTAGCTTTTGAAGAAA
  TGCGAAAGAGACTAGAGGAACAGCATGCCCAGCAGTTGTCACTCCTCATAGCTGAGCAGGAGAGGGAGCA
  GGAGCAACTGCAAAAGGAAATAGAAGAGCAGGAGAAGATGCTGAAGGAGAAGGCAGTTACGACGGACGTC
  TCTGACTTGAACAGTGCACTGGAGTGGAGACAAAGAACCGATTCTGCTTTGCTAGAAACCATGCTGTCCC
  AAGTGGACTCACTCCAGACTTCAAACAACTCTGGCTTCATAACTTCTGCCCTGCAGTACAGCTTCGGCTC
  TGCAGGTGAAGCACCGTTCTACCTCTGGGGATCACTGACCAGTGGCGTGACCAGAGTCTCAGGAACAAGG
  CCTTGTGGACGGGCCCAAGCTAAATGGTCTCAGGTTTTTAATCCAGAAATACACGCAAAATTTAATAAAA
  TAACTGCCGTGGCAAAAGGGTTTCTTACTCGTAAACTTATGCAAACAGACAAACTGAAGCAGCTTCGACA
  AACTGTAAAAGACACCATGGAATTCATACGAAGTTTTCAGTCAGAAGCCCCTCTGAAGAGAGGTGTCGTC
  TCAGCCCAAGATGCTTCCCTTCAGGAACGAGTGTTAGCTCAGTTGCGAGCTGCTCTGTATGGTATTCATG
  ACATCTTCTTCGTAATGGATGCAGCCGAGAGAATGTCAATCCTCCATCATGATCGAGAAGCCCGCAAAGA
  GAAACTGCTCCGGCAAATGGATAAGATGAAAAGTCCACGAGTGGCGCTTTCAGTGGCAACACAGAAGTCC
  CTGGACAGGAAGAAGTTCATGAAAGTTGCTGAGATGGGAATGCCAAATAAGAAGTTCCTGCTTAAACAAA
  ACCCCTCAGAAACAAGAGTCCTTCAGCCAAACCAAGGACAGAATGCACCCGTTCATAGGCTACTTAGTAG
  ACAAGGAACCCCTAAGACTTCAGTGAAGGGGGTTGTGCAAAATAGACAGAAGCCTTCACAGAGCAGAGTG
  CCTAACAGAGCGCCTGTGTCAGGAGCATATGCAGGAAAAACCCAAAGAAAGCGGCCAAATGTTGCGACAA
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211433 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEYEEFCEKALGRAQEASLSTGSFLPAQAESVSLIRFHGVAVLSPLLTIEKRKKIQEEKQKALDVQSRK
  QANRKKALLTRVQEILENVQVRKAPNASDFDQWATETIYSNPEVTDLNVPVRVPNSLPSPTEHCTSVKLE
  KITGLLPVNNEDQQTPKRVGLPGDSEVSGSLRQCESPESRQAEDGAALRLSSASPQETIISDVLGKEEQD
  PSCLAEVTPDPYIMSLQNLMKRSKEYVERELSSRSLRNSLKRSVNETHSDRENDAAKASDCVKEKAPPMP
  IGRHCGSAIPDKPSLNKSNVLLQGASQASSMGTAGLASFSKIDLPAGAAPPAAPDAGSDFTVIPTFVTEN
  KVKSLKGPYAKLPSPEPSMSPTMHRRHSRSASACQILINNPVNACELSPKGKEEAVDRTAPAAAETTNES
  ETVPKSPTDLTGVCSSNVSATKITSESTREMVVGKPSQRQQALGAHLGNNVTVERSAMEGPFIADDRGAQ
  KVDGTCMAVPKLHELQPSSQCVSSQTLEDVCELKSASLLAKNSCNLQMELNKSYDVKHPSPLLTQTQTSR
  QQMDTPPVFRGNEQFVDNSFEKVKRRLDLDVDSLQKENCPYIITAGVAEQERDRLLERRYPKGFVHINKN
  KMLETSPKEGQELLKSKMLAFEEMRKRLEEQHAQQLSLLIAEQEREQEQLQKEIEEQEKMLKEKAVTTDV
  SDLNSALEWRQRTDSALLETMLSQVDSLQTSNNSGFITSALQYSFGSAGEAPFYLWGSLTSGVTRVSGTR
  PCGRAQAKWSQVFNPEIHAKFNKITAVAKGFLTRKLMQTDKLKQLRQTVKDTMEFIRSFQSEAPLKRGVV
  SAQDASLQERVLAQLRAALYGIHDIFFVMDAAERMSILHHDREARKEKLLRQMDKMKSPRVALSVATQKS
  LDRKKFMKVAEMGMPNKKFLLKQNPSETRVLQPNQGQNAPVHRLLSRQGTPKTSVKGVVQNRQKPSQSRV
  PNRAPVSGAYAGKTQRKRPNVATI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182995
• ORF Size: 3015 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182995.1, NP_892040.1
• RefSeq Size: 5024 bp
• RefSeq ORF: 3015 bp
• Locus ID: 101565
• MW: 111.1 kDa