Tnk2 (NM_001110147) Mouse Tagged ORF Clone

CAT#: MR211437

Tnk2 (Myc-DDK-tagged) - Mouse tyrosine kinase, non-receptor, 2 (Tnk2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnk2
• Synonyms: Ack; Ack-1; Ack1; Cdgip; Pyk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211437 representing NM_001110147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCGGAGGAGGGAACGGGCTGGCTGTTGGAGCTGCTGTCCGAGGTGCAGCTACAACAGTATTTCC
  TGAGGCTTCGAGATGACCTCAACATTACCCGCCTATCTCATTTTGAATATGTCAAAAATGAAGACCTGGA
  AAAGATTGGCATGGGCCGGCCTGGCCAGAGGCGGCTGTGGGAGGCCGTGAAGAGGAGGAAGGCCATGTGC
  AAACGCAAGTCATGGATGAGCAAGGTGTTCAGTGGAAAGCGGTTGGAGGCCGAGTTCCCTTCCCAGCACT
  CTCAGAGCACCTTCCGGAAGCCCTCCCCCACCCCAGGGAGCCTGCCAGGGGAGGGGACCCTGCAGAGCCT
  CACCTGCCTCATTGGGGAGAAAGACCTACGCCTGCTGGAGAAGCTGGGGGATGGCTCCTTTGGCGTGGTG
  CGCAGGGGTGAATGGGACGCCCCCGCAGGGAAGACGGTGAGTGTGGCCGTCAAGTGCCTGAAGCCTGACG
  TGCTGAGCCAGCCAGAGGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCATTCACTAGACCACCG
  AAACCTCATTCGCTTGTATGGTGTGGTGCTCACACTACCCATGAAGATGGTGACAGAGCTGGCACCTCTG
  GGATCTTTGTTGGACCGCCTACGTAAACACCAAGGTCATTTCCTCTTGGGGACGCTGAGTCGCTACGCTG
  TGCAGGTGGCTGAGGGTATGGCCTACCTGGAGTCCAAGCGCTTCATTCACCGGGATCTGGCTGCTCGAAA
  CCTGCTTTTGGCTACCCGGGACCTGGTCAAGATTGGGGACTTCGGACTGATGCGAGCTCTGCCCCAGAAT
  GATGACCACTATGTCATGCAAGAACACCGCAAGGTGCCCTTTGCCTGGTGTGCCCCTGAGAGCCTGAAGA
  CACGGACTTTCTCCCATGCCAGTGACACCTGGATGTTTGGGGTCACACTGTGGGAGATGTTCACCTATGG
  CCAGGAGCCCTGGATTGGCCTCAATGGCAGCCAGATCCTGCATAAGATCGACAAGGAAGGGGAGCGCCTG
  CCCCGGCCGGAGGACTGCCCTCAAGACATCTACAATGTCATGGTCCAGTGCTGGGCCCACAAGCCAGAGG
  ACAGACCCACATTTGTGGCTCTTCGGGACTTCCTGCTGGAGGCTCAGCCCACTGACATGCGGGCTCTTCA
  GGACTTTGAGGAGCCAGATAAACTGCACATCCAGATGAATGACGTCATCACTGTCATCGAGGGAAGGGCT
  GAGAACTACTGGTGGCGTGGGCAGAATACGCGGACCCTGTGTGTAGGACCCTTCCCTCGAAATGTCGTGA
  CCTCCGTGGCTGGCCTGTCAGCCCAGGACATCAGCCAGCCTCTACAGAATAGCTTCATTCACACAGGACA
  TGGTGACAGTGACCCCCGCCACTGCTGGGGGTTCCCTGACAGGATCGATGAACTGTACCTGGGAAACCCC
  ATGGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGA
  AAAAGCCAACATACGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCT
  GAGGAAGCCAGCCCTGCCTCGAGGGCTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCA
  GACCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGC
  CCTGTGCACCCTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAG
  CCCCACACGGGCACTGCCACGACCTTTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCC
  CCGCCCCCTGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGC
  TAGTAGGTGCAGGCCTCCCTGCTGGGCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGC
  GCAGATGCCCCCTGCCCTGGAGGATAACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCA
  GTGCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCC
  AGCTGACCCCCTCCCCGACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCC
  TCGGCCCACGCGTCCACGTGTGGAGCTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCT
  GGACCTGCCTCGCCCCCCCGAGTGCCTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCC
  CCCTAGTGCCACCTGGCAGCTCTCCACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCAC
  CACTCAAAGCTTTGCCTCAGACCCTAAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCA
  GGCCCCTGCATCCTGCCCATTGTCCGCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTG
  AGCGCCCTCCTTACCTGGAACGCTATCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGC
  CCTGCCTGTGCCCCCGCTGTTGCCCCCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGA
  CCTATGCCTCAGGCCGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGC
  CACCATCCCTGAGGGCCACGGCTCGGCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCG
  GCCAGCAGACAAGGTCCAGATGGTGGAGCAGCTCTTTGGGCTGGGTCTTCGGCCACGGGTGGAGTGCCAC
  AAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTGTCACCTTCTGGGCTCCTGTGGCCCTG
  CTCATCACAAACGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211437 representing NM_001110147
  Red=Cloning site Green=Tags(s)
  
  MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNITRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMC
  KRKSWMSKVFSGKRLEAEFPSQHSQSTFRKPSPTPGSLPGEGTLQSLTCLIGEKDLRLLEKLGDGSFGVV
  RRGEWDAPAGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTLPMKMVTELAPL
  GSLLDRLRKHQGHFLLGTLSRYAVQVAEGMAYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQN
  DDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERL
  PRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRA
  ENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNP
  MDPPDLLSVELSTSRPTQHLGRVKKPTYDPVSEDPDPLSSDFKRLGLRKPALPRGLWLAKPSARVPGTKA
  DRSSGGEVTLIDFGEEPVVPTPRPCAPSLAQLAMDACSLLDKTPPQSPTRALPRPLHPTPVVDWDARPLP
  PPPAYDDVAQDEDDFEVCSINSTLVGAGLPAGPSQGETNYAFVPEQAQMPPALEDNLFLPPQGGGKPPSS
  VQTAEIFQALQQECMRQLQVPTGQLTPSPTPGGDDKPQVPPRVPIPPRPTRPRVELSPAPSGEEETSRWP
  GPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPHRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRA
  GPCILPIVRDGRKVSSTHYYLLPERPPYLERYQRFLREAQSPEEPAALPVPPLLPPPSTPAPAAPTATVR
  PMPQAAPDPKANFSTNNSNPGARPPSLRATARLPQRGCPGDGQEAARPADKVQMVEQLFGLGLRPRVECH
  KVLEMFDWNLEQAGCHLLGSCGPAHHKR
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110147
• ORF Size: 3024 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110147.1, NP_001103617.1
• RefSeq Size: 4313 bp
• RefSeq ORF: 3027 bp
• Locus ID: 51789
• MW: 112.2 kDa