Ank2 (NM_178655) Mouse Tagged ORF Clone

CAT#: MR211532

Ank2 (Myc-DDK-tagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ank2
• Synonyms: 100043364; AI835472; Ank-2; AW491075; Gm4392
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211532 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGAGGTCCTTGATGTTTCTGATGAAGAGGGTGATGACACTGTGACAGGTGATGGGGGAGAATACC
  TCAGGCCAGAAGATCTCAAGGAGCTCGGAGATGACTCACTGCCCAGCAGTCAGTTCCTGGATGGCATGAA
  CTACCTTCGGTACAGTCTGGAGGGAGGAAGATCCGACAGCCTCCGGTCCTTCAGTTCCGACAGGTCTCAC
  ACTCTGAGCCATGCATCGTACCTGAGGGACAGTGCCATGATTGACGACACGGTTGTGATCCCCAGCCACC
  AGGTGTCTGCGCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGTCTGAGCTGGGGCACTGAGAACTTAGA
  CAACGTGGCTCTTTCTTCCAGTCCTATTCATTCAGGTTTCCTAGTCAGTTTTATGGTGGATGCCCGTGGT
  GGTGCCATGCGAGGATGCAGACACAATGGACTCAGAATCATTATCCCACCTCGGAAATGCACAGCCCCAA
  CTCGCGTCACCTGCCGCCTTGTGAAACGCCATCGACTGGCAACAATGCCGCCCATGGTGGAAGGAGAAGG
  CCTGGCCAGCCGCCTGATTGAAGTCGGACCTTCGGGAGCTCAGTTTCTTGGGCCCGTGATCGTGGAGATC
  CCTCACTTCGCCGCTCTTCGAGGAAAGGAGAGGGAGCTGGTGGTCCTGCGCAGTGAAAATGGGGACAGCT
  GGAAAGAGCATTTCTGCGACTACACTGAGGATGAATTGAACGAAATCCTTAATGGCATGGATGAAGTGCT
  GGACAGTCCAGAAGACCTGGAAAAGAAACGAATCTGCCGCATCATCACTCGTGACTTCCCACAGTACTTT
  GCGGTGGTGTCTCGCATCAAACAGGACAGCAACCTGATTGGCCCCGAGGGTGGAGTACTGAGCAGCACAG
  TGGTGTCACAGGTGCAAGCCGTCTTCCCAGAGGGCGCACTCACCAAGCGCATTCGAGTAGGCCTACAGGC
  TCAACCTATGCATAGTGAATTGGTAAAGAAGATCTTAGGCAACAAAGCCACCTTCAGCCCAATAGTCACT
  TTGGAACCCAGGAGAAGAAAATTCCACAAGCCAATTACTATGACTATTCCTGTCCCCAAAGCTTCAAGTG
  ATGTCATGCTGAATGGTTTTGGGGGAGACGCACCAACCTTAAGATTACTGTGCAGCATAACAGGTGGAAC
  TACGCCTGCCCAGTGGGAAGACATCACAGGAACAACGCCACTAACATTTGTCAATGAGTGTGTTTCCTTT
  ACAACCAACGTGTCTGCCAGGTTCTGGCTGATAGACTGTCGACAGATTCAGGAGTCTGTTGCCTTTGCAT
  CACAAGTGTATAGAGAAATTATCTGTGTGCCATATATGGCCAAATTTGTAGTGTTTGCCAAGTCACATGA
  CCCCATTGAAGCCAGGTTGCGGTGTTTCTGTATGACAGATGACAAAGTAGATAAGACCCTTGAACAACAA
  GAAAACTTCTCTGAGGTGGCCAGGAGCAGGGATGTGGAGGTATTGGAAGGAAAACCTATTTATGTTGATT
  GTTTTGGCAACCTGGTTCCACTAACCAAGAGTGGCCAACATCATATATTCAGCTTTTTTGCCTTCAAAGA
  AAATAGACTTCCTCTCTTTGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCTCATTTATG
  AAGGAACCCAAATCCACAAGAGGACTGGTGCATCAAGCTATTTGCAACTTAAACATCACCCTGCCAATTT
  ATGCCAAGGAATCGGAGTCAGATCAAGAGCCGGAGGAAGAGATCGGTATGACATCCGAAAAAAATCCCCA
  AGATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCTGGACAGAA
  TTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAACTCCCTTC
  AAGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGATACCATCCT
  CATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAGAGCCCCTC
  CAGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGAAGGATTTT
  CAGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAAAGCGAGTC
  TAGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGGATTGCCTC
  CCCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGTTCAACAAG
  ATTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTGGAAGACCC
  TCAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTCCTGAGGGA
  ACAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACCTGAGGAGG
  CTTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACAGACGACCA
  GTCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTGACATACCC
  CCAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGTTACCCGGA
  AAATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGAATGCCTCA
  GGAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGAAGAGTGAC
  ACCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCAGGCCGAGC
  CAGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATGGAGAAGAG
  TCTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACAACAATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211532 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTEVLDVSDEEGDDTVTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSH
  TLSHASYLRDSAMIDDTVVIPSHQVSALAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARG
  GAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEI
  PHFAALRGKERELVVLRSENGDSWKEHFCDYTEDELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYF
  AVVSRIKQDSNLIGPEGGVLSSTVVSQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVT
  LEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSF
  TTNVSARFWLIDCRQIQESVAFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQ
  ENFSEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFM
  KEPKSTRGLVHQAICNLNITLPIYAKESESDQEPEEEIGMTSEKNPQDEQERMEERLAYIADHLGFSWTE
  LARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTILIECLTKINRMDIVHLLETNTEPL
  QERMGRSYAEIEQTITLDHSEGFSVLPDELCAAKEKKEQEASKESESSDHPPMVSEEDISVGYSTFQDCL
  PKTEGDSPAAALSPQMHQEPVQQDFSGKTQDQQEYYVTTPGAEVEDPQKATAVPDSLCKTPEDISTPPEG
  TKPCLQTPVTSERGSPIVQEPEEASEPKEESSPRKTSLVIVESTDDQSQVFERLDGDAAFQKGDDMPDIP
  PETVTEEEYVDENGHTVVKKVTRKIIRRYVSSDGTEKEEVTMQGMPQEPVNIEDGDNYSKVIKRVVLKSD
  TQQSEVTLSEPSVLSSTSQFQAEPVEGRRVSKVVKTTMVHGERMEKSLGDSSLATDLPSAKDDFEEDNNE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178655
• ORF Size: 3153 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178655.1, NM_178655.2, NM_178655.3, NP_848770.2
• RefSeq Size: 5790 bp
• RefSeq ORF: 3153 bp
• Locus ID: 109676
• MW: 117.4 kDa