Smarcc2 (NM_198160) Mouse Tagged ORF Clone

CAT#: MR211632

Smarcc2 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (Smarcc2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smarcc2
• Synonyms: 5930405J04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211632 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGCGGAAGAAGGACGGCGGCCCCAACGTGAAGTACTACGAGGCCGCGGACACCGTGACCCAGT
  TCGACAACGTGCGGCTCTGGCTCGGCAAGAACTACAAGAAGTACATACAAGCAGAACCGCCAACCAACAA
  GTCTCTGTCCAGCCTGGTGGTGCAGTTGCTCCAGTTTCAGGAAGAGGTTTTTGGCAAACATGTCAGCAAC
  GCACCGCTTACTAAACTGCCGATCAAATGTTTCCTAGATTTCAAAGCAGGAGGATCCCTCTGCCATATTC
  TTGCAGCTGCCTACAAATTCAAGAGTGACCAGGGATGGCGGCGTTACGATTTCCAGAATCCATCACGCAT
  GGACCGCAATGTGGAAATGTTCATGACCATTGAGAAGTCCTTGGTACAGAATAATTGCCTGTCACGACCT
  AACATTTTCCTCTGCCCAGAAATTGAGCCCAAACTGCTAGGGAAATTAAAAGACATTGTTAAGAGACACC
  AGGGAACCATCTCTGAGGATAAGAGCAATGCCTCCCATGTTGTGTATCCTGTCCCAGGGAACCTAGAAGA
  AGAGGAATGGGTACGGCCAGTCATGAAGAGGGATAAACAGGTTCTTCTGCACTGGGGCTACTATCCTGAC
  AGCTACGACACGTGGATCCCAGCGAGTGAAATTGAAGCATCTGTGGAGGACGCTCCCACTCCTGAGAAAC
  CGAGGAAGGTCCATGCGAAGTGGATCCTCGACACCGACACATTCAACGAGTGGATGAATGAGGAAGACTA
  CGAAGTCAGTGACGACAAAAGCCCAGTCTCCCGCAGGAAGAAGATCTCAGCCAAGACGCTGACAGACGAG
  GTAAACAGCCCAGATTCAGACAGACGAGACAAGAAGGGGGGCAACTATAAGAAGAGGAAGCGCTCTCCCT
  CTCCTTCACCCACCCCAGAGGCTAAGAAGAAAAACGCTAAGAAAGGACCCTCAACACCTTATACCAAGTC
  AAAGCGAGGCCACAGAGAAGAGGAACAAGAAGACCTGACAAAAGACATGGATGAGCCCTCTCCAGTCCCA
  AACGTGGAAGAGGTGACACTCCCCAAAACAGTCAACACTAAAAAGGACTCTGAGTCAGCCCCAGTCAAAG
  GCGGCACCATGACTGACCTGGATGAACAGGACGATGAAAGCATGGAGACCACCGGCAAGGACGAGGATGA
  GAACAGCACGGGCAACAAAGGCGAGCAGACGAAGAACCCGGACCTGCATGAGGACAATGTGACCGAGCAG
  ACCCACCACATCATCATCCCCAGCTACGCCGCCTGGTTTGACTACAACAGCGTCCATGCCATTGAACGGA
  GGGCTCTTCCTGAGTTCTTCAACGGCAAGAACAAGTCTAAGACTCCAGAGATCTACCTGGCGTATCGGAA
  CTTCATGATTGACACTTACCGACTGAATCCCCAGGAGTATCTAACATCTACTGCCTGTCGGCGGAATTTG
  GCGGGTGATGTCTGCGCTATCATGAGGGTCCATGCCTTCCTGGAACAGTGGGGTCTTATTAACTACCAGG
  TAGATGCTGAGAGCCGACCAACCCCAATGGGGCCTCCACCCACCTCTCACTTCCATGTCTTGGCGGACAC
  ACCATCAGGGCTGGTTCCTCTTCAGCCGAAGCCTCCACAGCAGAGCTCTGCTTCCCAGCAAATGCTGAAC
  TTCCCTGAGAAGGGCAAGGAGAAACCAGCAGACATGCAGAATTTTGGGCTGCGCACAGACATGTACACAA
  AGAAGAACGTCCCCTCCAAGAGCAAAGCTGCAGCAAGTGCCACTCGGGAATGGACGGAGCAGGAGACTCT
  GCTGCTCCTGGAGGCTTTGGAAATGTACAAGGACGACTGGAACAAAGTATCTGAGCACGTGGGAAGCCGC
  ACGCAGGACGAGTGCATCTTGCATTTTCTCCGCCTTCCCATTGAAGACCCATACCTGGAGGACTCGGAGG
  CTTCTCTAGGCCCTCTGGCCTACCAACCCATCCCCTTCAGTCAGTCAGGCAACCCTGTTATGAGCACCGT
  TGCCTTCCTGGCCTCTGTCGTCGATCCCCGAGTTGCCTCTGCTGCTGCGAAGTCAGCCCTAGAAGAGTTC
  TCAAAAATGAAGGAAGAGGTGCCCACAGCTTTGGTGGAAGCCCACGTGCGTAAGGTCGAAGAAGCGGCCA
  AAGTCACAGGCAAGGCCGACCCAGCCTTTGGTCTGGAGAGTAGCGGCATCGCAGGGACTGCCTCTGATGA
  GCCTGAGCGCATTGAGGAAAGCGGGACTGAGGAGGCACGGCCAGAGGGCCAGGCAGCAGATGAGAAGAAG
  GAGCCTAAGGAACCACGGGAAGGAGGGGGCGCTGTGGAGGAAGAAGCAAAGGAGGAAATAAGTGAGGTCC
  CCAAGAAAGATGAAGAGAAAGGGAAAGAAGGTGACAGTGAGAAGGAGTCTGAGAAGAGTGACGGGGACCC
  GATAGTTGATCCTGAGAAAGACAAGGAACCAACAGAAGGGCAGGAGGAAGTGCTAAAGGAAGTGGCAGAG
  CCAGAGGGGGAGAGGAAAACCAAGGTGGAGCGTGACATTGGTGAAGGCAACCTGTCCACAGCTGCAGCCG
  CAGCCCTGGCCGCTGCTGCAGTCAAGGCCAAGCACTTGGCTGCAGTTGAGGAGAGAAAGATCAAGTCTTT
  GGTGGCTCTGCTGGTAGAGACCCAAATGAAGAAACTAGAGATCAAACTCCGACATTTTGAGGAGCTGGAG
  ACAATAATGGACCGGGAGCGAGAGGCGCTGGAATACCAGAGGCAGCAGCTCCTGGCCGACCGGCAAGCCT
  TCCACATGGAGCAGCTGAAGTATGCAGAGATGAGGGCCCGGCAGCAGCACTTCCAGCAGATGCACCAGCA
  GCAGCAGCAGCAGCCACCAACCTTGCCCCCAGGCTCCCAGCCCATACCTCCCACCGGGGCTGCTGGACCA
  CCTACAGTCCATGGTCTAGCTGTGCCTCCAGCCGCTGTGGCCTCTGCCCCTCCTGGCAGTGGGGCCCCTC
  CTGGAAGCTTGGGCCCTTCTGAACAGATTGGGCAGGCAGGGACAACTGCAGGGCCACAGCAGCCACAACA
  AGCTGGAGCCCCTCAGCCTGGGGCAGTCCCACCAGGGGTACCCCCCCCTGGACCCCATGGCCCCTCACCG
  TTCCCCAACCAACCAACTCCTCCCTCAATGATGCCAGGGGCAGTGCCAGGCAGCGGGCACCCAGGCGTGG
  CGGACCCAGGCACCCCGCTGCCTCCAGACCCCACAGCTCCAAGCCCAGGCACAGTCACCCCTGTGCCACC
  TCCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211632 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVRKKDGGPNVKYYEAADTVTQFDNVRLWLGKNYKKYIQAEPPTNKSLSSLVVQLLQFQEEVFGKHVSN
  APLTKLPIKCFLDFKAGGSLCHILAAAYKFKSDQGWRRYDFQNPSRMDRNVEMFMTIEKSLVQNNCLSRP
  NIFLCPEIEPKLLGKLKDIVKRHQGTISEDKSNASHVVYPVPGNLEEEEWVRPVMKRDKQVLLHWGYYPD
  SYDTWIPASEIEASVEDAPTPEKPRKVHAKWILDTDTFNEWMNEEDYEVSDDKSPVSRRKKISAKTLTDE
  VNSPDSDRRDKKGGNYKKRKRSPSPSPTPEAKKKNAKKGPSTPYTKSKRGHREEEQEDLTKDMDEPSPVP
  NVEEVTLPKTVNTKKDSESAPVKGGTMTDLDEQDDESMETTGKDEDENSTGNKGEQTKNPDLHEDNVTEQ
  THHIIIPSYAAWFDYNSVHAIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNL
  AGDVCAIMRVHAFLEQWGLINYQVDAESRPTPMGPPPTSHFHVLADTPSGLVPLQPKPPQQSSASQQMLN
  FPEKGKEKPADMQNFGLRTDMYTKKNVPSKSKAAASATREWTEQETLLLLEALEMYKDDWNKVSEHVGSR
  TQDECILHFLRLPIEDPYLEDSEASLGPLAYQPIPFSQSGNPVMSTVAFLASVVDPRVASAAAKSALEEF
  SKMKEEVPTALVEAHVRKVEEAAKVTGKADPAFGLESSGIAGTASDEPERIEESGTEEARPEGQAADEKK
  EPKEPREGGGAVEEEAKEEISEVPKKDEEKGKEGDSEKESEKSDGDPIVDPEKDKEPTEGQEEVLKEVAE
  PEGERKTKVERDIGEGNLSTAAAAALAAAAVKAKHLAAVEERKIKSLVALLVETQMKKLEIKLRHFEELE
  TIMDREREALEYQRQQLLADRQAFHMEQLKYAEMRARQQHFQQMHQQQQQQPPTLPPGSQPIPPTGAAGP
  PTVHGLAVPPAAVASAPPGSGAPPGSLGPSEQIGQAGTTAGPQQPQQAGAPQPGAVPPGVPPPGPHGPSP
  FPNQPTPPSMMPGAVPGSGHPGVADPGTPLPPDPTAPSPGTVTPVPPPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198160
• ORF Size: 3300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198160.1, NM_198160.2, NP_937803.1
• RefSeq Size: 4622 bp
• RefSeq ORF: 3300 bp
• Locus ID: 68094
• MW: 121.4 kDa