Upf1 (NM_030680) Mouse Tagged ORF Clone

CAT#: MR211651

Upf1 (Myc-DDK-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Upf1
• Synonyms: B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211651 representing NM_030680
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTGGAGGCGTACGGCCCCAGCTCGCAAACACTCACCTTCTTGGACACTGAGGAGGCCGAGCTGC
  TCGGCGCCGACACCCAGGGCTCCGAGTTCGAATTCACCGACTTCACCCTTCCCAGCCAGACGCAGACGCC
  CCCCGGCGGCCCCGGCGGCGCGGGAGGCCCGGGCGGAGCGGGCGCAGGCGGCGCGGCCGGCCAGCTCGAC
  GCACAAGTTGGACCAGAGGGCATCTTGCAAAATGGGGCTGTGGATGACAGTGTGGCCAAGACCAGCCAGC
  TGCTAGCTGAGCTGAACTTCGAGGAAGATGAAGAGGACACATACTACACTAAGGACCTCCCAGTCCACGC
  CTGCAGTTACTGTGGAATCCATGATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGC
  AATGGCCGAGGAAATACTTCTGGCAGCCACATTGTGAATCACCTCGTGAGGGCAAAATGCAAGGAAGTGA
  CGCTGCACAAGGACGGGCCTCTGGGCGAGACCGTGCTGGAGTGCTACAACTGTGGCTGCCGCAACGTCTT
  CCTGCTGGGCTTCATCCCTGCGAAGGCCGACTCTGTGGTGGTGCTGTTGTGCAGGCAGCCCTGTGCCAGC
  CAGAGCAGCCTGAAGGACATCAACTGGGACAGCTCACAGTGGCAGCCCCTAATCCAGGACCGGTGCTTTC
  TGTCATGGCTGGTCAAGATTCCCTCTGAGCAGGAGCAGCTGCGAGCACGGCAGATCACGGCACAGCAGAT
  CAACAAGCTGGAAGAGCTCTGGAAGGAAAATCCTTCAGCCACTCTGGAGGACCTGGAGAAGCCAGGCGTA
  GACGAGGAGCCACAGCACGTGCTCCTGCGTTACGAGGATGCTTACCAGTACCAGAACATCTTCGGGCCAC
  TGGTCAAGCTGGAGGCTGACTATGACAAGAAGTTGAAGGAGTCACAGACTCAAGATAACATCACGGTCAG
  GTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTTCTTCACTTTGCCCAAGACTGACTCTGACATGCGG
  CTCATGCAGGGTGATGAGATCTGTCTGCGGTACAAAGGGGATCTGGCGCCCCTGTGGAAGGGGATTGGCC
  ACGTCATCAAGGTTCCTGATAATTATGGTGATGAGATTGCTATTGAGCTCCGCAGCAGCGTGGGTGCCCC
  TGTGGAAGTGACCCACAACTTCCAAGTGGATTTTGTGTGGAAGTCAACCTCTTTTGATAGGATGCAGAGT
  GCACTGAAGACCTTCGCTGTGGACGAGACCTCTGTGTCAGGGTATATTTACCACAAGCTGCTGGGCCACG
  AGGTGGAGGATGTGGTCATCAAGTGCCAGCTGCCAAAGCGCTTCACAGCTCAGGGGCTCCCTGACCTCAA
  CCACTCTCAGGTGTATGCTGTGAAGACCGTGCTGCAGAGACCACTCAGCCTCATCCAGGGCCCTCCAGGC
  ACAGGCAAGACTGTGACATCAGCCACTATTGTCTACCACCTTGCTCGGCAGGGCAATGGGCCTGTACTGG
  TTTGTGCTCCAAGTAACATCGCTGTGGACCAGCTCACAGAGAAGATCCACCAGACAGGACTGAAGGTCGT
  ACGCCTCTGTGCCAAGAGCCGTGAGGCCATTGACTCCCCAGTGTCCTTCCTGGCTTTGCACAACCAGATC
  AGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGCAGCAGCTAAAGGATGAGACAGGCGAGCTGTCAT
  CTGCAGATGAGAAGCGGTACCGGGCGCTTAAGCGCACAGCTGAGAGAGAACTTCTCATGAATGCAGATGT
  CATATGCTGCACATGTGTGGGTGCTGGTGACCCGAGGCTGGCCAAGATGCAGTTCCGTTCCATCCTCATT
  GACGAGAGCACCCAGGCCACTGAGCCTGAGTGCATGGTGCCTGTAGTCCTTGGGGCCAAGCAGCTAATCC
  TCGTCGGTGACCACTGCCAGCTGGGCCCAGTGGTGATGTGCAAGAAGGCAGCCAAGGCCGGACTGTCACA
  ATCGCTCTTCGAGCGCTTGGTGGTGCTGGGCATCCGGCCCATCCGCCTGCAGGTGCAATACCGCATGCAC
  CCTGCACTCAGCGCCTTTCCGTCCAACATCTTCTACGAGGGCTCATTGCAGAATGGCGTCACTGCAGCGG
  ATCGTGTCAAAAAAGGCTTTGACTTCCAGTGGCCACAACCTGACAAGCCTATGTTCTTCTACGTGACGCA
  GGGCCAGGAGGAGATTGCCAGCTCTGGCACATCCTACCTCAACAGGACGGAGGCAGCCAATGTGGAGAAG
  ATAACTACGAAGCTGTTGAAGGCAGGTGCAAAGCCTGACCAGATCGGCATCATCACCCCCTACGAGGGCC
  AGCGCTCTTACTTGGTGCAGTACATGCAGTTCAGCGGCTCCCTGCACACAAAGCTCTACCAGGAAGTGGA
  AATTGCCAGTGTGGACGCCTTCCAGGGCCGGGAGAAGGACTTCATCATTCTGTCCTGCGTGCGCGCCAAT
  GAACATCAGGGCATTGGGTTCCTAAACGACCCCCGGCGTCTGAATGTGGCTCTCACCAGAGCAAGATATG
  GCGTGATCATTGTGGGTAACCCAAAGGCCCTGTCGAAGCAGCCCCTGTGGAATCACCTGCTGAGCTACTA
  CAAGGAACAGAAGGCGCTAGTGGAAGGGCCGCTCAACAACCTACGTGAGAGCCTCATGCAGTTCAGCAAG
  CCTCGCAAACTTGTCAACACTGTCAACCCGGGTGCCCGCTTCATGACTACTGCCATGTACGATGCCCGTG
  AGGCCATCATCCCCGGGTCTGTCTATGACCGCAGCAGCCAGGGCCGGCCCTCGAACATGTACTTCCAGAC
  CCATGACCAGATTAGTATGATCAGCGCAGGCCCCAGCCACGTGGCTGCCATGAACATCCCTATTCCCTTC
  AACTTGGTCATGCCTCCCATGCCGCCACCTGGCTACTTCGGACAGGCCAACGGGCCGGCAGCTGGTCGGG
  GCACCCCAAAAACCAAGACTGGCCGTGGGGGCCGCCAGAAGAACCGCTTTGGGCTTCCTGGGCCCAGCCA
  GACCACCCTTCCCAACAGCCAGGCCAGCCAGGACGTGGCCTCCCAGCCCTTTTCACAGGGTGCCCTCACA
  CAGGGTTACGTGTCCATGAGCCAGCCCTCTCAGATGAGCCAGCCTGGCCTCTCCCAGCCAGAACTGTCCC
  AGGACAGCTACCTCGGTGATGAGTTTAAATCACAGATTGACGTGGCACTCTCACAAGACTCCACATACCA
  GGGAGAGCGGGCATACCAGCACGGCGGGGTCACCGGGCTGTCCCAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211651 representing NM_030680
  Red=Cloning site Green=Tags(s)
  
  MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGAGGPGGAGAGGAAGQLD
  AQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFC
  NGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCAS
  QSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGV
  DEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAFFTLPKTDSDMR
  LMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQS
  ALKTFAVDETSVSGYIYHKLLGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPG
  TGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQI
  RNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILI
  DESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMH
  PALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEK
  ITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRAN
  EHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLSYYKEQKALVEGPLNNLRESLMQFSK
  PRKLVNTVNPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQISMISAGPSHVAAMNIPIPF
  NLVMPPMPPPGYFGQANGPAAGRGTPKTKTGRGGRQKNRFGLPGPSQTTLPNSQASQDVASQPFSQGALT
  QGYVSMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030680
• ORF Size: 3339 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030680.1, NM_030680.2, NM_030680.3, NP_109605.2
• RefSeq Size: 4598
• RefSeq ORF: 3342
• Locus ID: 19704
• MW: 123.1 kDa