Polr1b (NM_009086) Mouse Tagged ORF Clone

CAT#: MR211691

Polr1b (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polr1b
• Synonyms: 128kDa; D630020H17Rik; RPA2; RPA116; RPA135; Rpo1-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211691 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTCGACGGCCGGTGGCGGAACCTGCCCAGCGGGCCCAGCCTAAAGCATTTAACCGACCCCTCGT
  ACGGGATTCCCCCAGAGCAGCAAAAGGCAGCTTTGCAGGACCTGACGCGGGCGCACGTGGACTCCTTCAA
  CTACGCGGCGCTGGAGGGGCTGAGCCACGCGGTGCAGGCCATACCTCCCTTTGAATTTGCTTTCAAAGAT
  GAGCGCATATCTCTTACCATTGTGGATGCTGTCATCAGTCCACCCTCAGTGCCCAAAGGGACCATCTGCA
  AAGATCTCAACGTTTATCCAGCTGAGTGTCGTGGCCGAAAGAGCACCTACCGAGGGAGGCTGACGGCTGA
  TATCAGCTGGGCCGTGAATGGAGTCCCCAAAGGGATCATTAAACAGTTTCTCGGCTATGTTCCCATCATG
  GTGAAGTCCAAGCTTTGCAACTTATACAACCTTCCTCCTCGAGTCCTCATTGAGCACCATGAGGAGGCAG
  AGGAAATGGGCGGTTATTTTATAATCAATGGCATAGAAAAAGTCATCCGCATGTTGATTGTGCCCCGGAG
  AAATTTCCCCATCGCCATGGTAAGGCCGAAATGGAAAAGCAGAGGACTCGGCTACACTCAGTTTGGGGTC
  TCCATGCGCTGTGTGAGAGAAGAGCACTCCGCCGTGAACATGAACCTGCACTATGTGGAGAACGGCACGG
  TTATGTTAAACTTTATTTACCGCAAAGAGCTCTTTTTCCTTCCTTTGGGATTTGCACTTAAGGCACTCGT
  GAGCTTTTCTGATTATCAGATTTTCCAGGAGCTTATCAAGGGCAAAGAGGAGGACTCTTTCTTTAGGAAT
  TCTGTTTCTCAGATGCTGCGGATTGTCATAGAAGAGGGCTGTCACTCACAGAAGCAGGTTCTTAACTATC
  TGGGAGAATGCTTCCGAGTGAAGCTCAGCCTTCCTGATTGGTACCCTAACGTGGAAGCTGCCGAGTTCCT
  GCTTAACCAGTGCATCTGCATCCACCTGCAGTCCAACACTGACAAGTTCTACCTGCTCTGCCTCATGACC
  CGGAAGCTGTTCGCTTTAGCCAGAGGGGAGTGCATGGACGACAACCCTGATAGCCTAGTGAACCAAGAGG
  TCTTCAGCCCTGGGCAGCTCTTCCTGATGTTTCTGAAGGAAAAAATGGAAAACTGGCTAGTGTCTATTAA
  AATAGTTTTAGATAAGAGGGCTCAGAAGGCCAATGTTTCCATAAACAACGAAAATTTGATGAAGATTTTT
  AGTATGGGAACAGAGCTAACAAGACCATTTGAATATCTTCTTGCTACTGGGAATCTGCGTTCTAAAACAG
  GTCTCGGCTTCCTGCAAGATTCTGGCCTGTGTGTTGTGGCTGACAAGCTGAACTTCCTTCGCTACCTCTC
  CCATTTCCGCTGTGTGCACAGAGGGGCTGCCTTTGCCAAGATGAGGACCACCACCGTGCGCAGGCTGCTG
  CCAGAATCCTGGGGCTTCCTCTGCCCTGTGCACACTCCAGACGGGGCGCCTTGTGGGCTGTTGAACCACT
  TGACTGCCGTGTGTGAGGTTGTTACCAAGTTTGTGTACACGGCATCTATTCCAGCCTTGCTCTGTGGCTT
  AGGAGTCACTCCTGTTGATACAGCGCCATGTCGACCGTACAGTGACTGCTACCCTGTCCTGCTGGATGGC
  GTCATGGTGGGCTGGGTGGATAAGGACCTTGCTCCTGAAGTTGCAGACACTCTCCGTCGATTCAAGGTGT
  TGAGAGAAAAAAGAATTCCTCCCTGGATGGAGGTGGCCCTGATTCCCATGACAGGAAAACCAAGTCTGTA
  CCCAGGTCTGTTCCTCTTTACCACTCCCTGCAGGCTGGTGAGGCCTGTGCAGAACTTGGAACTGGGCAGA
  GAAGAGCTCATTGGAACCATGGAACAGCTCTTCATGAACGTCGCCATCTTTGAGGACGAGGTGTTTGGTG
  GCATCTCCACACACCAGGAGCTCTTCCCTCACAGCCTGCTGAGCGTGATCGCCAACTTCATCCCCTTCTC
  GGATCACAACCAGAGTCCTCGGAACATGTACCAGTGCCAGATGGGTAAGCAGACCATGGGCTTTCCGCTG
  CTCACTTACCAAAACCGATCAGATAATAAACTCTATCGACTCCAAACCCCTCAGAGCCCTCTAGTGAGAC
  CGTGCATGTATGATTTCTATGACATGGACAACTATCCGATCGGGACAAACGCCATTGTAGCTGTGATCTC
  CTACACTGGCTATGATATGGAGGACGCCATGATTGTCAACAAGGCTTCCTGGGAACGAGGCTTCGCTCAT
  GGAAGCGTCTACAAGTCTGAGTTCATAGACCTCTCAGAGAAGTTTAAGCAGGGAGAGGATAACCTGGTGT
  TCGGGGTCAAGCCTGGTGACCCTCGGGTGATGCAGAAGCTGGACGATGATGGGTTGCCATTCATAGGCGC
  AAAGCTGGAGTATGGCGACCCTTACTACAGCTACCTCAACCTTAACACTGGGGAAGGCTTCGTGGTTTAC
  TACAAGAGTAAAGAAAACTGTGTTGTGGACAACATCAAAGTGTGTAGTAACGACATGGGCAGTGGGAAGT
  TCAAGTGTATCTGCATCACAGTCCGCATCCCCCGGAACCCAACCATTGGAGATAAGTTTGCCAGCCGACA
  TGGTCAGAAGGGCATTTTGAGCAGATTGTGGCCAGCCGAGGACATGCCTTTCACTGAGAGTGGCATGATG
  CCGGACATTCTGTTTAATCCTCACGGGTTTCCCTCCCGTATGACCATAGGTATGTTAATCGAGAGCATGG
  CTGGGAAGTCTGCAGCTTTGCACGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCCGAGGAGAACTC
  TGCCTTAGAGTACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAACTTCTACGGCACCGAGAGACTGTAT
  AGTGGCATCAGTGGGATGGAGCTGGAGGCTGACATCTTCATTGGTGTGGTTTATTACCAGCGCTTACGAC
  ACATGGTGTCAGACAAATTTCAAGTCAGAACAACTGGAGCCAGGGACAAAGTCACCAACCAGCCCCTCGG
  GGGACGAAATGTTCAGGGTGGAATCCGCTTTGGGGAGATGGAGCGGGATGCTCTGTTGGCACACGGTACG
  TCTTTCCTTCTTCATGACCGGCTTTTCAACTGCTCTGACCGTTCTGTCGCTCACATGTGTGTAGAGTGTG
  GCAGTTTGCTTTCTCCGCTACTTGAGAAGCCTCCTCCGTCTTGGTCTGCGATGCGCAACAGAAAGTACAA
  CTGCACTGTCTGTGGCCGCAGTGACACCATCGACACCGTCTCTGTGCCGTATGTCTTCCGGTACTTTGTA
  GCTGAGCTGGCAGCCATGAACATCAAAGTGAAACTGGATGTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211691 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDVDGRWRNLPSGPSLKHLTDPSYGIPPEQQKAALQDLTRAHVDSFNYAALEGLSHAVQAIPPFEFAFKD
  ERISLTIVDAVISPPSVPKGTICKDLNVYPAECRGRKSTYRGRLTADISWAVNGVPKGIIKQFLGYVPIM
  VKSKLCNLYNLPPRVLIEHHEEAEEMGGYFIINGIEKVIRMLIVPRRNFPIAMVRPKWKSRGLGYTQFGV
  SMRCVREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFRN
  SVSQMLRIVIEEGCHSQKQVLNYLGECFRVKLSLPDWYPNVEAAEFLLNQCICIHLQSNTDKFYLLCLMT
  RKLFALARGECMDDNPDSLVNQEVFSPGQLFLMFLKEKMENWLVSIKIVLDKRAQKANVSINNENLMKIF
  SMGTELTRPFEYLLATGNLRSKTGLGFLQDSGLCVVADKLNFLRYLSHFRCVHRGAAFAKMRTTTVRRLL
  PESWGFLCPVHTPDGAPCGLLNHLTAVCEVVTKFVYTASIPALLCGLGVTPVDTAPCRPYSDCYPVLLDG
  VMVGWVDKDLAPEVADTLRRFKVLREKRIPPWMEVALIPMTGKPSLYPGLFLFTTPCRLVRPVQNLELGR
  EELIGTMEQLFMNVAIFEDEVFGGISTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPL
  LTYQNRSDNKLYRLQTPQSPLVRPCMYDFYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAH
  GSVYKSEFIDLSEKFKQGEDNLVFGVKPGDPRVMQKLDDDGLPFIGAKLEYGDPYYSYLNLNTGEGFVVY
  YKSKENCVVDNIKVCSNDMGSGKFKCICITVRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMM
  PDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLY
  SGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPLGGRNVQGGIRFGEMERDALLAHGT
  SFLLHDRLFNCSDRSVAHMCVECGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDTIDTVSVPYVFRYFV
  AELAAMNIKVKLDVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009086
• ORF Size: 3408 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009086.2, NP_033112.2
• RefSeq Size: 4017 bp
• RefSeq ORF: 3408 bp
• Locus ID: 20017
• MW: 128.2 kDa