Mtus1 (NM_001005863) Mouse Tagged ORF Clone

CAT#: MR211806

Mtus1 (Myc-DDK-tagged) - Mouse mitochondrial tumor suppressor 1 (Mtus1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtus1
• Synonyms: AI481402; ATBP135; Atip1; B430010I23Rik; B430305I03Rik; C85752; Cctsg1-440; MD44; mKIAA1288; MTSG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211806 representing NM_001005863
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGATGATAATTCAGATAGGACAGAAGATGGATCGCGTTATGTATTTATCCGAGATAAAAACTCGA
  ATCCATCTGAATACTACCAAACATCGCTATCTGCCCAGTGCCCTTCAGTGAGTCACGGGGATTGGAACTC
  TGACAACCCAGATGCCATGGTGGTCGATTATGAAATGGATCCTGCGGTGGACAGTAGTGAAAGTGTATCT
  TTAAGCCATCAGTGTGTTGAAGAACTTGCTTACCCAGAGCCTTCAAGTGATTTCATGGGGAAGCATGAGT
  TTACAATGTACAGTGAGCTTACCTGCCAGTCTCCTGCACTTGTGAACACAGGGAAGCCCCAAGACCTGCA
  CAGTAACTGTGACTCCCTAGAGGCAATCCAGGATGAGAAGTTTGATCCCCTTAAGCCCTGTGAGTGCAGG
  TCTGATGACGATTATGCTTGTGGAGATTCCCCTGAAGTTTTGGAGCTAAAACAAACATATGGCATGAAAG
  TGGACACGGCTAACTACACCTTTATAGCACGCCATGATATTGAACAGGGTCAGCCTTTGCATGCTCCTGG
  AGGTTTGCAGACAACAGTGAGAGACAGAAATGCTTTGTCCTCCTGCGGTCGGACACCACCCCATTCCTCT
  AAGATGTATGTGAGAGGGGTTAATTATAATCGAGAGAACTTTGAAAACCTCCAAGCCACACCATCCAAGA
  CCCTAAACACAACCTTTACAGTGATCTCTGATGTGCTGATGCAAACTGACAGTCCAGACGTTGGAGTTCA
  GGGTCAGAATTCTTTAGGAAATGTCACCAAAGAGTACACAGATGGGACAAGGCGAGGACTCATTGGAGAA
  AAAGAGATACAAGCTGTGACACTGGTTTCGGATGGCATGGAGGTCCCCAACGGATCTGCATCACAGGAGT
  TCTATTGTGTATCTGAAGATGACCCAAACAGTGAGACACATTCACACGGCCCGTATGCTCAACAGGAAAT
  GGGCCAAAATTTAAGAGGAACACTGCCCAACTGTCATGTCGATGGTGAATGCCCAGTACTGGTGCCAGCT
  TTTGAGAAGAGTAAAACCAGAGTCCTGGGCTCTGAGTGTAAAGTCACTGTGACTGAAGACCCACACATCG
  ATTCCCACGACAATGACTCAGACATCCAAAGTAGTACTGAAGAGCTGACCCTGAGAAGTGTTTCAGGGCA
  GAGGGGTTCACCTTATGAAATGGGTTGGGGCGAGAACGGCGGAGCCATTTGCACAGATAAAGCAGGTTGC
  ATGTCAACACCAGTTGAGCAACCCCCCAATCTGAGCTTCAGACTTGAACCTGCTGAAGTGAAGAAGTACA
  ATAATGTGGAGAATGGACCAAGAGATGCTAAACGTGCACCAAACTTGAAAGGGGAACCAACAAATATGCC
  AAAACCTAATTTGGGAAAATCAGCTACCAAAACAAATACCACTGTAGGCAGTAAAGTCAGGAAGACAGAA
  ATTATAAGTTATCCTACACCAAACTTCAAAAACATTAAAGCCAAAGTTATATCCAGATCAGTGTTGCAAC
  CCAAAGACACTTCCATAATGAAGGACACACCCAGCCCTCAGGTAACAGGGGGCTCTTCACCATCACCAGG
  GCCTTCTAAACACTTGACAATGATGAACAAAGCACCAAGATCTGACTTTAAAGCCAGCAAAAAAGCAGAA
  ATCCCAATTAACAAGACACACAAGCAACAGTTTAATAAACTCATTACCAGCCAGGCTGCGCAAGTTACAA
  CTCATTCTAAAAATGCTTCACTCGGGGTTCCAAGAACAACATCTGCCACGAAATCGAATCAGGAGAATGT
  TGACAAGACAGGTTCTCCTCATGCAGGGAGTGAGACCGGGTCTGTAGCTGCCTTCTTCCAGAAGATCAAA
  GGCATTCTCCCCGTGAAAATGAAAAGCTCAGAGTGTTTGGAAGTGACTTACGTTTCCCACATCGATCAGA
  TTAGCCCTGAAAAGGGTGAGCAGGACGGTGAGGCTCCCATGGAAAAGCAAGAGCTGGGAAAACAAGCCAC
  GAATGAGATTTTTGAATCCAAGTCTCTATTGGTGGGCTCTGCACCCAAAACTTCCACCACGCCAGGAAGG
  AGCAGTTCCAAGCCAGACTCGCGGAGCCTGAGGAAAACACCAGGTCTAAAAGCCAAAGTGGGTCCCACTG
  CTGCCTGCCTGAGGAGGAAGAGTGAGAGTAGAACCCTAGGCTCCGACAGGGCCTTATCGCCTCAGAGGAT
  CAGGCGCGTGTCCGGCTCTGGTGGTCATGCTGCCATCAACAAATATGAAGAGAAACCTCCCAAACAAGCA
  TTTCAGAATGGGTCAGGACCCTTGTATCTGAAGCCTTTGGTACCCAGAGCTCACTCGCACTTGCTCAAAA
  CTTCTCCAAAAGGTCCTTCAAGGAAGAGTCTGTTTACAGCTTTTAATTCAGTTGAAAAGGGCAGGCAGAA
  GAATCCCAGGAGCCTATGCATCCAGACCCAGACAGCTCCAGATGTGCTGTCCTCCGAGAGAACGCTTGAG
  TTGGCCCAATACAAGACAAAATGTGAAAGCCAAAGTGGATTCATCCTGCACCTCAGACAGCTTCTTTCCC
  GTGGTAACACCAAGTTTGAAGCGCTGACCGTTGTGATCCAGCACCTCCTGTCTGAGCGGGAGGAAGCACT
  GAAGCAACACAAAACCCTCTCTCAAGAACTTGTCAGCCTCCGGGGAGAGCTAGTTGCTGCTTCAAGCGCC
  TGTGAGAAGCTAGAAAAGGCTAGGACTGACTTACAGACAGCGTATCAAGAATTTGTCCAGAAACTAAACC
  AGCAGCATCAGACAGACCGGACGGAACTGGAGAACCGGCTGAAGGACTTATACACCGCAGAGTGTGAGAA
  GCTTCAGAGCATTTACATTGAGGAGGCAGAAAAATATAAAACTCAACTGCAAGAGCAGTTTGACAACTTA
  AACGCCGCCCATGAGACCACTAAGCTTGAGATTGAAGCTAGCCACTCGGAGAAGGTGGAATTGCTGAAGA
  AGACCTATGAAACCTCCCTTTCAGAAATCAAGAAGAGCCATGAGATGGAGAAGAAGTCACTGGAGGATCT
  GCTTAATGAGAAGCAGGAATCGCTGGAGAAACAAATCAATGATCTGAAGAGTGAAAACGATGCTTTAAAC
  GAAAGGTTGAAATCAGAGGAGCAAAAGCAACTGTCAAGAGAGAAGGCGAATTCAAAAAACCCTCAGGTCA
  TGTATCTGGAGCAAGAACTAGAAAGCCTGAAGGCTGTGTTAGAGATCAAGAATGAGAAGCTGCACCAGCA
  GGACATGAAGCTAATGAAGATGGAAAAGCTGGTGGACAATAACACAGCATTGGTTGACAAGCTGAAGCGA
  TTCCAGCAGGAAAACGAGGAGTTAAAAGCTCGCATGGACAAACACATGGCAATTTCAAGGCAACTTTCCA
  CCGAGCAGGCCGCGCTGCAGGAGTCCCTTGAGAAGGAGTCGAAGGTCAACAAGAGACTGTCCATGGAGAA
  CGAGGAACTTCTGTGGAAACTGCACAACGGAGACCTGTGCAGCCCCAAGAGATCCCCCACCTCCTCGGCC
  ATCCCTTTCCAGTCCCCCAGGAATTCTGGTTCCTTCTCCAGCCCCAGCATCTCACCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211806 representing NM_001005863
  Red=Cloning site Green=Tags(s)
  
  MNDDNSDRTEDGSRYVFIRDKNSNPSEYYQTSLSAQCPSVSHGDWNSDNPDAMVVDYEMDPAVDSSESVS
  LSHQCVEELAYPEPSSDFMGKHEFTMYSELTCQSPALVNTGKPQDLHSNCDSLEAIQDEKFDPLKPCECR
  SDDDYACGDSPEVLELKQTYGMKVDTANYTFIARHDIEQGQPLHAPGGLQTTVRDRNALSSCGRTPPHSS
  KMYVRGVNYNRENFENLQATPSKTLNTTFTVISDVLMQTDSPDVGVQGQNSLGNVTKEYTDGTRRGLIGE
  KEIQAVTLVSDGMEVPNGSASQEFYCVSEDDPNSETHSHGPYAQQEMGQNLRGTLPNCHVDGECPVLVPA
  FEKSKTRVLGSECKVTVTEDPHIDSHDNDSDIQSSTEELTLRSVSGQRGSPYEMGWGENGGAICTDKAGC
  MSTPVEQPPNLSFRLEPAEVKKYNNVENGPRDAKRAPNLKGEPTNMPKPNLGKSATKTNTTVGSKVRKTE
  IISYPTPNFKNIKAKVISRSVLQPKDTSIMKDTPSPQVTGGSSPSPGPSKHLTMMNKAPRSDFKASKKAE
  IPINKTHKQQFNKLITSQAAQVTTHSKNASLGVPRTTSATKSNQENVDKTGSPHAGSETGSVAAFFQKIK
  GILPVKMKSSECLEVTYVSHIDQISPEKGEQDGEAPMEKQELGKQATNEIFESKSLLVGSAPKTSTTPGR
  SSSKPDSRSLRKTPGLKAKVGPTAACLRRKSESRTLGSDRALSPQRIRRVSGSGGHAAINKYEEKPPKQA
  FQNGSGPLYLKPLVPRAHSHLLKTSPKGPSRKSLFTAFNSVEKGRQKNPRSLCIQTQTAPDVLSSERTLE
  LAQYKTKCESQSGFILHLRQLLSRGNTKFEALTVVIQHLLSEREEALKQHKTLSQELVSLRGELVAASSA
  CEKLEKARTDLQTAYQEFVQKLNQQHQTDRTELENRLKDLYTAECEKLQSIYIEEAEKYKTQLQEQFDNL
  NAAHETTKLEIEASHSEKVELLKKTYETSLSEIKKSHEMEKKSLEDLLNEKQESLEKQINDLKSENDALN
  ERLKSEEQKQLSREKANSKNPQVMYLEQELESLKAVLEIKNEKLHQQDMKLMKMEKLVDNNTALVDKLKR
  FQQENEELKARMDKHMAISRQLSTEQAALQESLEKESKVNKRLSMENEELLWKLHNGDLCSPKRSPTSSA
  IPFQSPRNSGSFSSPSISPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005863
• ORF Size: 3630 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005863.1, NM_001005863.2, NP_001005863.1
• RefSeq Size: 6554 bp
• RefSeq ORF: 3633 bp
• Locus ID: 102103
• Cytogenetics: 8 A4
• MW: 134.9 kDa