Dctn1 (BC066061) Mouse Tagged ORF Clone

CAT#: MR211869

Dctn1 (Myc-DDK-tagged) - Mouse dynactin 1 (cDNA clone MGC:86152 IMAGE:6849060)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dctn1
• Synonyms: p150, Glued
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211869 representing BC066061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTACGGAGGCAAGCGCCCGGCCCCTGCGGGTTGGCTCCCGCGTGGAGGTGATTGGGAAGGGCCACC
  GAGGCACTGTGGCCTATGTTGGAGCCACACTCTTTGCCACTGGCAAATGGGTGGGCGTGATTCTGGATGA
  AGCAAAAGGCAAAAATGATGGCACTGTCCAGGGAAGGAAGTATTTCACATGTGATGAAGGCCACGGCATC
  TTTGTACGCCAGTCCCAGATCCAAGTATTTGAAGATGGAGCAGATACTACTTCCCTAGAGACTCCTGATT
  CTTCTGCTTCAAAGGTCCTCAAGAGAGAGGGAGCCGATGCAGCTGCAAAGACCAGCAAACTGCGGGGACT
  GAAGCCTAAGAAGGCACCGACAGCCCGAAAGACCACAACTCGACGGCCCAAGCCTACTCGCCCAGCCAGC
  ACTGGGGTGGCTGGGCCCAGTAGCTCCCTTGGCCCCTCTGGCTCAGCGTCAGCCGGGGAACTAAGCAGCA
  GTGAGCCCAGCACCCCAGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACACCGGCCCTCACCTCTCC
  TGGAGCAGCACCCCCACTTCCATCTCCCTCTAAGGAAGAGGAAGGGCTGAGGGCTCAGGTACGGGACCTG
  GAGGAGAAGCTGGAGACCCTGCGCCTAAAACGCTCAGAAGACAAAGCAAAGCTGAAAGAGCTGGAGAAGC
  ACAAGATCCAGCTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCAGACCTGCAGCG
  GCGCCTCAAGGAGGCTCGGAAGGAAGCCAAGGAGGCGCTAGAGGCAAAGGAACGCTACATGGAGGAGATG
  GCCGACACAGCCGACGCTATCGAGATGGCCACTCTGGACAAGGAGATGGCTGAAGAGCGCGCTGAGTCTC
  TGCAGCAAGAGGTGGAGGCACTGAAGGAACGGGTAGACGAGCTCACCACAGACCTGGAGATTCTCAAGGC
  TGAAATCGAAGAGAAAGGCTCTGATGGGGCCGCATCAAGCTACCAGCTCAAGCAGCTGGAGGAGCAGAAT
  GCCCGCCTGAAGGATGCCCTGGTGAGGATGCGAGACCTCTCTTCCTCAGAGAAGCAGGAGCACGTGAAGC
  TGCAGAAACTCATGGAAAAGAAAAACCAGGAGCTGGAGGTCGTGCGGCAGCAGCGCGAGCGTCTTCAGGA
  GGAGCTGAGCCAGGCTGAGAGCACCATCGATGAGCTCAAAGAGCAGGTGGACGCCGCTCTGGGAGCCGAG
  GAGATGGTGGAGATGCTGACCGACCGGAACCTGAATCTAGAGGAGAAAGTGCGGGAGTTACGGGAGACTG
  TGGGGGACTTGGAAGCCATGAACGAGATGAACGATGAGCTGCAGGAGAACGCACGGGAGACGGAGCTGGA
  ACTCCGAGAGCAGCTGGACATGGCGGGCGCCCGAGTGAGGGAAGCGCAGAAGCGAGTGGAAGCCGCCCAG
  GAGACAGTCGCCGACTACCAGCAGACCATCAAGAAGTACCGCCAGTTGACTGCCCACCTACAGGATGTCA
  ATCGGGAGCTGACAAACCAGCAGGAAGCGTCTGTAGAGAGGCAGCAGCAGCCGCCGCCAGAGACTTTTGA
  TTTCAAAATCAAGTTTGCTGAGACCAAGGCTCATGCCAAGGCCATTGAGATGGAGTTGAGACAGATGGAA
  GTTGCCCAGGCCAACCGGCACATGTCCCTGCTGACAGCCTTTATGCCTGACAGCTTCCTTCGGCCAGGTG
  GAGACCACGACTGTGTCCTGGTGCTGCTGCTCATGCCCCGACTCATTTGCAAGGCAGAGCTCATCCGGAA
  GCAGGCCCAGGAGAAGTTTGACCTGAGCGAGAACTGTTCGGAGCGGCCCGGGCTGCGGGGAGCTGCCGGG
  GAGCAGCTGAGCTTTGCTGCTGGACTGGTGTACTCGCTGAGTCTGCTGCAGGCCACGCTGCACCGCTATG
  AGCATGCCCTCTCTCAGTGCAGTGTGGACGTGTATAAGAAGGTCGGCAGCCTGTACCCCGAGATGAGCGC
  CCACGAGCGCTCCTTAGATTTCCTCATTGAGCTGCTGCACAAGGATCAGCTGGATGAGACTGTCAACGTG
  GAGCCCCTCACCAAGGCCATCAAGTATTACCAGCATCTGTACAGCATCCACCTCGCTGAACAACCCGAGG
  ACTCCACCATGCAGCTGGCTGACCACATCAAGTTCACCCAGAGTGCCCTGGACTGCATGGGCGTGGAGGT
  GGGGCGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCAACAGATATTGCCCTTCTTCTCCGAGACCTG
  GAAACATCATGTAGTGACACCCGTCAGTTCTGCAAGAAGATCCGAAGGCGGATGCCGGGGACGGATGCTC
  CTGGGATCCCAGCAGCGCTGGCCTTTGGCTCACAGGTATCCGACACACTCCTGGACTGCAGGAAGCACTT
  GACGTGGGTGGTAGCTGTTCTGCAGGAGGTGGCAGCTGCAGCCGCCCAGCTTATTGCCCCCTTGGCAGAG
  AACGAGGGGCTGCCTGTGGCTGCACTGGAGGAGCTGGCCTTCAAAGCAAGCGAGCAGATCTACGGGAGCC
  CCTCCAGCAGCCCCTATGAGTGTCTACGCCAGTCATGCACCATCCTCATCAGCACGATGAACAAGCTGGC
  CACAGCCATGCAAGAAGGCGAGTATGACGCAGAGCGACCCCCGAGCAAGCCTCCTCCGGTTGAACTTCGG
  GCTGCAGCCCTGCGTGCAGAGATCACAGATGCTGAAGGTCTGGGTTTGAAGCTTGAGGATCGAGAGACAG
  TTATCAAGGAGTTAAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTGAGTGAGGCCAACGTGCGGCTCAG
  CCTCCTGGAGAAGAAGTTGGACAGCGCTGCCAAGGATGCAGACGAGCGAATCGAGAAAGTTCAGACACGG
  CTGGACGAGACTCAGACCCTGCTGCGGAAGAAGGAGAAAGACTTTGAGGAGACAATGGACGCACTCCAGG
  CTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAGCTCAAGCAGCGCCTGAACAGCCAGTCCAAGCGCAC
  AATCGAGGGGCTCCGGGGCCCCCCTCCGTCAGGCATCGCTACCCTGGTCTCTGGCATCGCTGGTGAGGAA
  CCACAGCGAGGGGGCGCTCCTGGGCAGGCTCCGGGCGCCTTGCCAGGCCCGGGGCTGGTGAAGGACTCCC
  CACTGCTGCTTCAGCAGATCTCTGCTATGAGGCTACACATCTCTCAGCTCCAGCATGAGAACAGCATCCT
  CAGAGGAGCCCAGATGAAGGCGTCCTTGGCAGCTCTGCCCCCTCTGCATGTTGCAAAGCTTTCCCTCCCA
  CCCCATGAGGGCCCCGGTGGTAACCTAGTGGCTGGGGCACTGTACCGCAAGACCAGCCAGCTCCTGGAGA
  AACTAAACCAGCTGAGTACCCACACCCACGTGGTGGATATCACTCGGAGCAGCCCAGCTGCCAAGAGCCC
  GTCAGCTCAGCTTATGGAACAAGTGGCTCAGCTCAAGTCCCTGAGTGACACCATTGAGAAGCTCAAGGAT
  GAGGTCCTCAAGGAGACAGTGACTCAGCGCCCTGGAGCCACTGTGCCCACCGACTTTGCCACTTTCCCTT
  CATCTGCCTTCCTCAGGGCCAAGGAAGAGCAGCAAGATGACACAGTCTACATGGGCAAAGTGACCTTTTC
  ATGCGCGGCAGGCCTAGGACAGCGACACCGCCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGT
  CGCCTCATCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211869 representing BC066061
  Red=Cloning site Green=Tags(s)
  
  MSTEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGI
  FVRQSQIQVFEDGADTTSLETPDSSASKVLKREGADAAAKTSKLRGLKPKKAPTARKTTTRRPKPTRPAS
  TGVAGPSSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPALTSPGAAPPLPSPSKEEEGLRAQVRDL
  EEKLETLRLKRSEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEM
  ADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQN
  ARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAE
  EMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQ
  ETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQME
  VAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFDLSENCSERPGLRGAAG
  EQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNV
  EPLTKAIKYYQHLYSIHLAEQPEDSTMQLADHIKFTQSALDCMGVEVGRLRAFLQGGQEATDIALLLRDL
  ETSCSDTRQFCKKIRRRMPGTDAPGIPAALAFGSQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAE
  NEGLPVAALEELAFKASEQIYGSPSSSPYECLRQSCTILISTMNKLATAMQEGEYDAERPPSKPPPVELR
  AAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTR
  LDETQTLLRKKEKDFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEE
  PQRGGAPGQAPGALPGPGLVKDSPLLLQQISAMRLHISQLQHENSILRGAQMKASLAALPPLHVAKLSLP
  PHEGPGGNLVAGALYRKTSQLLEKLNQLSTHTHVVDITRSSPAAKSPSAQLMEQVAQLKSLSDTIEKLKD
  EVLKETVTQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGLGQRHRLVLTQEQLHQLHS
  RLIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066061
• ORF Size: 3792 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC066061.1
• RefSeq Size: 4220
• RefSeq ORF: 3794
• Locus ID: 13191
• MW: 154.7 kDa