Polr1a (NM_009088) Mouse Tagged ORF Clone

CAT#: MR212069

Polr1a (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide A (Polr1a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polr1a
• Synonyms: 194kDa; 2900087K15Rik; 3010014K16Rik; mRPA1; RPA194; Rpo1-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212069 representing NM_009088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGCTGGCCTCCAAGCACACACCATGGAGGCGGCTTCAGGGCATTTCCTTCGGAATGTATTCGGCCGAAG
  AGCTCAAGAAGTTAAGTGTTAAATCCATTACAAATCCTCGATACGTGGACTACCTGGGAAACCCTTCAGC
  AAATGGCCTGTATGATTTGGCTTTGGGTCCTGCAGATTCCAAAGAAGTATGCGCTACCTGCGTGCAGGAC
  TTCAACAACTGCTCTGGACACCTGGGCCACATTGATCTCCCGCTCACAGTCTACAACCCTTTCCTCTTTG
  ATAAACTCTACCTGCTGCTTCGAGGCTCCTGTTTGAGTTGCCACATGCTGACTTGTCCTCGGGCTGCAAT
  TTACCTGTTGATCTCCCAGCTGAGGGTCCTGGAAGTTGGAGCCCTGCAAGCAGTCTACGAGCTTGAGCGA
  ATTCTCAGCAGGTTTCTGGAAGAAACTGGAGATCCTTCCGCCTTCGAAATTCAAGAAGAGTTAGAAGAAT
  ACACAAGCAAAATTCTGCAGAACAACCTCTTGGGGTCCCAGGGCACACATGTGAAAAATGTGTGCGAGAG
  CAGAAGCAAACTTGTCGCTCAGTTCTGGAAGACACACATGGCTGCGAAGCAGTGTCCACACTGCAAGACG
  GGACGGTCGGTGGTTCGAAAGGAGCACAATAGTAAACTAATCATCACATACCCAGCCACGGTGCACAAGA
  AATCAGACCAGGAGGGCACAGAACTCCCAGAAGGAGTGCCGGAAGCTCCAGGAATTGACAAGGCTCAGAT
  GGGGAAACGAGGGTACCTGACCCCAAGCAGCGCCCAAGAACACCTTTTCGCCATTTGGAAGAATGAAGGA
  TTCTTTCTGAATTATCTTTTTTCGGGATTGGATGATATTGGCCCAGAGTCCAGTTTCAACCCCAGTATGT
  TCTTTCTAGACTTCATAGTCGTACCGCCATCCAGGTACCGTCCCGTCAATCGCCTGGGTGACCAGATGTT
  CACTAATGGCCAGACAGTAAACTTGCAAGCTGTCATGAAGGATGCAGTTTTGATCCGGAAACTTCTGGCG
  TTGATGGCCCAAGAACAGAAACTGCCCTGTGAGATGACAGAACTCACCATAGACAAGGAAAATGACTCCT
  CAGTGGCTATCGACCGATCCTTCCTGGGTTTGCTTCCAGGCCCGTCTCTCACAGATAAACTTTACAACAT
  TTGGATTCGCCTTCAGAGCCACGTCAATATTGTGTTTGACAGTGAGATGGACAAATTGATGTTGGAAAAG
  TACCCTGGCATAAGACAGATCCTAGAGAAGAAGGAAGGCCTGTTCCGGAAGCACATGATGGGAAAGCGAG
  TTGACTACGCAGCCCGCTCGGTCATCTGCCCAGACATGTACATCAACACCAATGAGATCGGAATCCCCAT
  GGTATTTGCCACAAAACTGACTTACCCTCAGCCAGTTACCCCCTGGAACGTGCAGGAACTGAGACAGGCA
  GTCATCAATGGACCCAATGTGCACCCAGGAGCCTCCATGGTCATCAACGAGGATGGCAGTCGCACAGCCC
  TGAGCTCAGTGGATGCTGCGCAGCGGGAAGCTGTGGCCAAACAGCTCCTGACACCAGCCACAGGGGCTCC
  GAAGCCTCAGGGGACAAAAGTTGTGTGCCGGCATGTGAAAAATGGGGACATCCTCCTCCTGAACCGACAG
  CCTACCTTGCACAGACCCTCCATCCAGGCCCACCGTGCCCGCATTCTGCCTGAAGAAAAAGTCCTACGCC
  TTCACTATGCCAACTGCAAGGCCTACAATGCTGACTTTGATGGAGATGAGATGAACGCCCACTTCCCCCA
  GAGTGAGTTGGGCCGAGCTGAAGCCTATGTCCTGGCCTGCACTGATCAGCAGTATCTTGTTCCCAAGGAT
  GGCCAGCCATTGGCAGGACTGATCCAAGATCACATGGTTTCAGGTGCAAACATGACCATTCGGGGATGCT
  TTTTCACCCGGGAACAGTACATGGAACTGGTGTACCGAGGGCTCACAGACAAAGTGGGACGCGTGAAGCT
  CTTTCCTCCCGCCATCCTGAAGCCCTTCCCACTGTGGACAGGAAAACAGGTTGTGTCGACCCTCCTCATA
  AACATAATTCCAGAGGACTACGCCCCCCTGAACTTATCTGGAAAGGCAAAAATTGGCAGCAAAGCCTGGG
  TGAAGGAAAAGCCACGGCCTATCCCTGACTTTGACCCGGACTCCATGTGTGAGTCCCAGGTGATCATCAG
  GGAAGGAGAGCTGCTCTGCGGAGTGCTGGACAAGGCACACTATGGGAGCTCTGCCTACGGCCTGGTCCAC
  TGCTGCTATGAGATCTATGGGGGTGAGACCAGCGGCAGGGTTCTCACCTGCCTAGCCCGCCTCTTCACTG
  CCTACCTCCAGCTCTATAGAGGTTTCACCTTGGGTGTAGAAGACATTTTGGTTAAGCCAAATGCAGATGT
  GGTGAGACAGCGTATCATTGAAGAATCTACCCAGTGTGGACCCCAGGCTGTCAAGGCTGCATTGAGCCTG
  CCAGAAACTGCATCATGTGATGAAATCCAAGGGAAATGGCAGGATGCCCATCTGAGCAAAGACCAGAGGG
  ATTTTAACATGATTGATATGAAGTTCAAGGAGGAAGTGAACCATTACAGCAATGAGATTAACAAGGCATG
  TATGCCTCTTGGCTTGCACAGACAGTTCCCCGAAAACAACCTGCAGATGATGGTGCAGTCAGGAGCCAAA
  GGGTCAACCGTGAACACGATGCAAATCTCTTGCCTGCTGGGCCAAATTGAATTGGAAGGTCGGAGACCCC
  CACTGATGGCGTCTGGCAAGTCACTGCCCTGCTTTGAGCCTTACGAGTTCACCCCCAGGGCTGGTGGCTT
  TGTCACTGGAAGGTTTCTTACCGGTATCAGGCCTCCTGAGTTCTTCTTCCACTGCATGGCAGGACGAGAA
  GGCCTGGTGGACACTGCTGTCAAAACCAGCCGTTCTGGCTATCTCCAAAGGTGTATCATCAAGCACCTGG
  AGGGGCTGGTCATCCAGTACGATCTGACGGTCCGTGACAGCGATGGCAGCGTGGTCCAGTTCCTGTATGG
  GGAGGACGGCCTGGACATCCCCAAGACACAGTTCCTACAGCCCAAGCAGTTCCCCTTTCTGGCCGGCAAT
  TATGAGGTGATAATGAAATCCAAGCATCTTCATGAAGTTTTATCCAGAGCAGATCCCCAGAAAGTGCTCG
  GCCACATCAAAGCCATTAAAAAATGGCATCACAAGCATTCCGGTGCCCTGCTGAGGAAAGGCGCCTTCCT
  CAGTTTTTCCCAGAAGATCCAGGCAGCTGTGAAAGCTTTGAACCTAAAGGGCAGTATCCAGAATGGCCGC
  AGCCCCGAGACCCAGCAGATGTTACAGATGTGGTATGACTTGGATGAAGAGAGCCGATGGAAATACCAGA
  AGAGGGCTGCCCCTTGTCCCGACCCTAGCCTTTCTGTGTGGCGCCCAGACATCTACTTTGCATCTGTGTC
  TGAAACATTTGAAAAGAAGATTGATGACTTCAGTCAGGAATGGGCAGCTCAAGCAGAGAGGAGTTACAAG
  AAATCAGAGCTTTCCCTCGACAGGCTGAGAACCTTACTGCAGCTGAAGTGGCAGCGCTCACTGTGTGACC
  CAGGCGAGGCTGTTGGCCTCCTGGCTGCCCAGAGCATCGGAGAGCCCTCCACACAGATGACACTGAACAC
  CTTCCACTTTGCAGGCAGAGGCGAGATGAACGTCACCCTGGGCATTCCAAGATTGAGGGAGATTCTCATG
  GTGGCCAGTGCCAACATCAAAACGCCCATGATGAGTGTGCCTGTGTTCGACACCAAGAAAGCATTGAAGA
  AAGTGAAGAGTCTAAAGAAGAGGCTCACCAGAGTGTGCCTGGGGGAGGTATTGCAGAAAGTTGACATCCA
  GGAGTCCTTCTGTATGGGAGAAAAACGGAATAAGTTCCAGGTGTATGAGCTCCGGTTTCAGTTCCTGCCA
  CATGCATATTACCAGCAGGAGAAGTGCCTGAGACCCGAGGACATCTTGCACTTCATGGAAACCAGATTCT
  TTAAGCTACTAATGGAAGCTATCAAAAAGAAGAAAAACAAAGCATCAGCCTTCAGAAATGTGAATTCGAG
  GAGAGCTACACAGAAGGACCTCAATGACACTGAGGACTCGGGGAGGAGTCAGAGAGAGGAGGAAAGGGAT
  GAGGAAGAGGAGGGGAACATTGTGGATGCTGAAGCTGAGGAGGGCGATGCAGACGCTTCTGACACCAAAC
  GCAAGGAGAAGCAAGAAGAAGAGGTTGACTATGAGAGTGAGGAAGAAGGAGAGGAGGAAGAGGAGGAGGA
  GGTACAAGAAGAAGGGAACATCAAAGGCGACGGTGTCCACCAGGGCCATGAGCCAGATGAGGAAGAACAT
  TTAGGCCTCGAGGAGGAGGAGTCATCCCAGAAGCCTCCCCGCCGCCACTCCAGGCCTCAGGGAGCTGAGG
  CCATAAAACGCCGGATCCAGGCTGTACGGGAGTCCTACTCGTTCATCGAGGACTACCAGTATGACACAGA
  GGAGAGCCTGTGGTGTCAGGTGACTGTGAAGCTCCCACTGATGAAGATTAATTTTGACATGAGCTCCTTG
  GTTGTGTCCTTGGCCCACAAAGCCATTGTCTATACGACCAAGGGCATCACTCGGTGCCTCCTGAACGAAA
  CGACCAACAGTAAGAATGAGAAGGAGCTTGTACTGAACACGGAGGGAATCAACCTTCCCGAGCTGTTCAA
  GTACTCCGAGATACTGGACCTGCGCCGCCTCTACTCCAATGACATCCACGCCATGGCCAATACTTATGGC
  ATCGAGGCTGCGTTGCGGGTGATAGAGAAGGAGATTAAAGATGTGTTTGCTGTGTATGGCATTGCAGTTG
  ATCCTCGCCATCTCTCCCTGGTGGCTGATTACATGTGTTTTGAGGGGGTTTATAAGCCACTCAATCGCTT
  TGGGATCCAGTCAAGCTCCTCCCCTTTGCAGCAGATGACATTTGAAACCAGCTTCCAGTTTCTGAAACAG
  GCCACCATGATGGGATCCCATGATGAACTCAAGTCACCTTCCGCCTGCCTCGTGGTGGGAAAGGTTGTCA
  AAGGCGGGACAGGCCTGTTTGAGCTCAAGCAGCCCCTAAGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212069 representing NM_009088
  Red=Cloning site Green=Tags(s)
  
  MLASKHTPWRRLQGISFGMYSAEELKKLSVKSITNPRYVDYLGNPSANGLYDLALGPADSKEVCATCVQD
  FNNCSGHLGHIDLPLTVYNPFLFDKLYLLLRGSCLSCHMLTCPRAAIYLLISQLRVLEVGALQAVYELER
  ILSRFLEETGDPSAFEIQEELEEYTSKILQNNLLGSQGTHVKNVCESRSKLVAQFWKTHMAAKQCPHCKT
  GRSVVRKEHNSKLIITYPATVHKKSDQEGTELPEGVPEAPGIDKAQMGKRGYLTPSSAQEHLFAIWKNEG
  FFLNYLFSGLDDIGPESSFNPSMFFLDFIVVPPSRYRPVNRLGDQMFTNGQTVNLQAVMKDAVLIRKLLA
  LMAQEQKLPCEMTELTIDKENDSSVAIDRSFLGLLPGPSLTDKLYNIWIRLQSHVNIVFDSEMDKLMLEK
  YPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQA
  VINGPNVHPGASMVINEDGSRTALSSVDAAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQ
  PTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKD
  GQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLI
  NIIPEDYAPLNLSGKAKIGSKAWVKEKPRPIPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVH
  CCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSL
  PETASCDEIQGKWQDAHLSKDQRDFNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMVQSGAK
  GSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGRE
  GLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLAGN
  YEVIMKSKHLHEVLSRADPQKVLGHIKAIKKWHHKHSGALLRKGAFLSFSQKIQAAVKALNLKGSIQNGR
  SPETQQMLQMWYDLDEESRWKYQKRAAPCPDPSLSVWRPDIYFASVSETFEKKIDDFSQEWAAQAERSYK
  KSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILM
  VASANIKTPMMSVPVFDTKKALKKVKSLKKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLP
  HAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKKNKASAFRNVNSRRATQKDLNDTEDSGRSQREEERD
  EEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEEVQEEGNIKGDGVHQGHEPDEEEH
  LGLEEEESSQKPPRRHSRPQGAEAIKRRIQAVRESYSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSL
  VVSLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYG
  IEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQ
  ATMMGSHDELKSPSACLVVGKVVKGGTGLFELKQPLR
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-NotI Cloning Scheme for this gene
• ACCN: NM_009088
• ORF Size: 5151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009088.1, NM_009088.2, NM_009088.3, NP_033114.3
• RefSeq Size: 6145
• RefSeq ORF: 5154
• Locus ID: 20019
• MW: 194.1 kDa