Pi4ka (NM_001001983) Mouse Tagged ORF Clone
CAT#: MR212108
- TrueORF®
Pi4ka (Myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka)
"NM_001001983" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pi4ka |
Synonyms | Pik4ca |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR212108 representing NM_001001983
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCCAGTAGATTTTCATGGAATCTTCCAACTAGATGAAAGACGGAGAGATGCAGTGATTGCATTGG GCATTTTTCTCATTGAATCTGATCTTCAGCACAAAGATTGTGTTGTTCCTTATCTTCTTCGACTTCTCAG GGGTCTTCCAAAAGTGTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTAACCTCCCAGTTGCA GAGAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCACTTAGGGATG AGATTTTAGAGGCAATTTTGCAGGTTTTACATGTCCTTTTGGGAATGTGCCAGGCCTTGGAGATTCAAGA AAAAGAATACCTTTGCAAGTATGCGATCCCATGCCTGATAGGAATCTCAAGATCTTTTGGACGTTACAGC AATTCAGAAGAGTCTCTCCTCTCAAAGCTTTTCCCCAAAGTTTCTCCTCATTCCCTGAGAATCCCAGAAG AGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTTCGCTCCATCCTGCCCAGCAACCTGCTAACTGT CTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGT GGCATACCCCCTCCCAGCTCCCCTGGAGGATCTGCATTTCACTATTTTGAAGCTTCTTGCCTACCTGATG GGACTGCCTTGGAGCCTGAATACTACTTCTCTACCATCAGCTCCAGTTTTTCTATCTCTCCCCTGTTCAA TGGTATTACCTATAAGGAGTTCTGTATTCCACTGGAAATGCTTCGGGAACTCTTAAACTTGGTGAAGAAG ATTGTTGAGGAGCCTGTTCTCAAATCCTTAGATGCAATTGTAGCCGGCGTGATGGAGGCCAATCCCAGTG CTGATCTGTACTATACAACCTTCAGTGATCCCCTGTACCTGACCATGTTCAAGATGCTTCGAGATACTCT GTACTACATGAAAGATCTGCCCACTTCCTTTGTGAAAGAGATCCATGATTTTGTGTTGGAGCAGTTCAAC ATGAGCCAAGGGGAGCTCCAAAAGATTCTGCATGACGCAGATAGGATCCACAGTGAGATGAGCCCACTCA AACTGCGCTGCCAAGCAAACGCTGCCTGTGTGGACCTCATGGTGTGGGCTGTGAAGGATGAGCAGGGTGC AGAAAACCTTTGCATCAAGCTGTCTGAAAAGCTGCAGTCCAAGACATCCAGTAAGGTCATCATTGCCCAT TTGCCCTTGCTGATCTGCTGCCTACAGGGTCTGGGCCGCCTGTGTGAAAGGTTCCCAGTGGTGGTCCACT CAGTGACGCCATCTTTGAGAGACTTTCTGGTAATTCCATCCCCAGTGCTGGTGAAGCTCTATAAGTACCA CAGTCAGTACCACACAGTTGCTGGCAGTGATATAAAAATCAGTGTCACCAATGAGCATTCCGAGTCAACT CTGAATGTCTTGCCGGGCAAGAAGAACCAGCCATCCATGTATGAGCAGCTCCGAGACATCGCCATTGACA ACATCTGCAGGTGCCTGAAGGCCGGCCTCACCGTGGACCCTGTGATCGTGGAGGCCTTCCTGGCCAGCCT CTCCAACCGGCTCTACATTTCCCAGGAGAGTGACAAGGATGCTCACTTGATTCCTGACCATACCATCCGT GCCTTGGGACACATTGCAGTAGCTTTGAGGGACACTCCAAAGGTCATGGAGCCAATTCTGCAGATCCTGC AGCAGAAATTTTGCCAGCCCCCCTCACCCCTTGATGTGCTCATCATCGACCAGCTGGGCTGCCTGGTGAT CACTGGAAATCAATACATTTATCAGGAAGTGTGGAATCTCTTCCAGCAAATCAGTGTAAAGGCCAGTTCA GTTGTGTATTCAGCCACCAAAGACTATAAAGACCATGGCTACAGGCACTGCTCCCTAGCAGTGATCAATG CCCTGGCCAACATTGCAGCCAACATTCAGGAGGAGCATCTTGTGGACGAGCTGTTGATGAACCTGCTGGA ACTGTTTGTGCAGCTGGGGCTGGAGGGGAAAAGAGCCAGTGAGAGGGCCAGTGAAAAGGGGCCTGCCCTG AAGGCTTCCAGCAGTGCAGGAAATTTGGGCGTGCTCATTCCTGTAATAGCTGTGCTCACCCGACGATTGC CACCCATCAAAGAAGCTAAACCTAGGCTACAGAAGCTTTTCCGAGATTTCTGGCTCTACTCTGTGCTAAT GGGATTCGCTGTGGAAGGCTCAGGACTCTGGCCGGAAGAGTGGTATGAGGGAGTCTGTGAAATAGCTACC AAGTCACCTCTGCTTACCTTTCCCAGCAAAGAGCCTCTGCGATCAGTCCTCCAGTACAATTCAGCTATGA AAAACGATACGGTCACCCCTGCTGAACTGAGTGAGCTCCGAAGTACCATCATCAACTTGCTAGATCCTCC TCCTGAGGTGTCTGCCCTCATCAACAAGCTGGACTTTGCCATGTCTACCTATCTCTTGTCTGTGTATCGG CTGGAGTACATGAGGGTACTGCGTTCTACAGATCCAGATCGCTTCCAGGTAATGTTCTGCTACTTTGAGG ATAAAGCTATTCAAAAAGACAAATCTGGGATGATGCAGTGTGTCATTGCTGTTGCTGACAAAGTGTTTGA TGCTTTCCTGAACATGATGGCAGAAAAAGCTAAGACCAAGGAGAATGAAGAGGAGCTGGAGCGGCATGCA CAGTTTCTGCTGGTGAACTTCAACCACATTCATAAGAGGATAAGGAGAGTAGCTGACAAATACCTATCTG GTCTGGTGGATAAGTTTCCACACTTGCTCTGGAGCGGGACTGTGCTGAAGACCATGTTGGACATCCTGCA GACCCTGTCACTATCACTAAGTGCTGATATTCACAAGGACCAACCTTACTATGACATTCCTGATGCCCCC TATCGGATCACGGTTCCTGACACATACGAAGCCCGGGAGAGCATCGTGAAAGACTTTGCTGCGCGCTGTG GAATGATCCTACAGGAAGCCATGAAGTGGGCACCCACGGTCACTAAGTCCCACCTACAGGAATATCTGAA CAAGCATCAGAACTGGGTGTCAGGACTGTCTCAGCACACAGGATTGGCCATGGCCACTGAGAGCATCCTT CATTTTGCCGGCTACAACAAGCAGAACACAACTCTTGGGGCAACTCAGCTAACTGAGCGTCCGGCCTGTG TGAAGAAAGACTACTCCAACTTCATGGCATCCTTGAATTTGCGCAACCGCTATGCCGGCGAGGTACATGG AATGATTCGGTTCTCAGGTGCCACAGGCCAGATGTCTGACCTGAACAAAATGATGGTCCAAGATCTGATC ACAGCTTTGGACCACAGTCATCCTCAGCACTATACACAAGCCATGTTCAAATTGACGGCAATGCTCATTA GCAGCAAAGATTGTGATCCACAGCTCCTTCATCATCTGTGTTGGGGTCCCCTCCGAATGTTCAATGAGCA TGGCATGGAGACTGCACTAGCCTGCTGGGAGTGGCTGCTGGCTGGCAAGAATGGAGTGGAAGTGCCGTTT ATGCGGGAGATGGCAGGAGCCTGGCACATGACAGTAGAGCAGAAGTTCGGCCTCTTTTCTGTGGAAACAA AGGAAGCAGACCCCCTGGCAGCATCAGAAGCAAGTCAGCCAAGACCTTGTCCCCCCGAAGTGACACCTCA CTACATCTGGATCGATTTCCTGGTCCAGCGGTTTGAGATTGCCAAGTACTGTAGCTCTGACCAGGTGGAG ATCTTCTCAAGCCTGCTGCAGCGCTCCATGTCCCTCAATATTGGTGGAGCCAGGGGGAGCATGAACAGGC ATGTGGCAGCCATTGGACCACGTTTCAAACTGCTGACCTTGGGGCTGTCCCTCCTGCATGCTGATGTGGT TCCAAATGCAACCATTCGAAACGTGCTTCGAGAGAAGATATACTCAACAGCCTTTGACTATTTCAGTTGT CCACCAAAGTTTCCTACTCAAGGAGAAAAGAGGCTTCGTGAAGATATAAGCATAATGATTAAGTTTTGGA CTGCCATGTTCTCAGATAAGAAGTACCTGACTGCCAGCCAGCTCGTTCCCCCAGATAATCAGGACACCCG AAGCAACTTGGATATAACTGTGGGCTCTCGACAACAGGCTACCCAGGGCTGGATCAACACTTACCCCCTC TCCAGTGGCATGTCAACCATCTCCAAAAAGTCAGGTATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGC ACAAGTACTACATGAAACGCAGGACACTGCTGCTGTCACTATTGGCCACTGAGATCGAACGCCTCATCAC TTGGTACAACCCACTGTCAGCCCCAGAGCTAGAGCTGGACCAGGCAGGAGAGAACAGCGTTGCCAACTGG AGGTCCAAGTACATCAGCTTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTTGCCTGGAGCATCTCTC CATACCTCGCTGTGCAGCTGCCAGCCAGGTTCAAGAATACAGAAGCCATTGGAAATGAAGTGACCCGTCT CGTTCGCTTGGATCCTGGAGCTGTTAGTGATGTCCCTGAAGCCATCAAGTTCCTTGTCACCTGGCACACC ATCGATGCTGATGCTCCAGAGCTCAGCCATGTTTTATGCTGGGCACCCACGGACCCACCTACAGGCCTCT CCTACTTCTCCAGCATGTACCCACCACACCCTCTCACTGCACAGTATGGGGTAAAAGTCCTGCGATCCTT CCCTCCGGATGCCATTCTGTTCTACATCCCCCAGATTGTGCAGGCCCTCAGATATGACAAGATGGGCTAT GTGCGTGAGTACATTCTCTGGGCTGCAGCCAAATCCCAGCTTCTGGCACACCAGTTTATTTGGAACATGA AAACTAACATCTACCTTGATGAAGAAGGGCACCAGAAAGACCCTGACATCGGTGACCTCCTGGAGCAGTT AGTAGAAGAGATCACAGGGTCTCTATCAGGCCCAGCCAAAGATTTCTATCAACGGGAATTTGATTTCTTT AACAAGATCACAAACGTGTCAGCCATCATCAAGCCCTACCCTAAAGGCGATGAAAGGAAGAAGGCTTGTC TGTCAGCTCTGTCTGAAGTGAAGGTGCAGCCTGGCTGCTACCTGCCCAGCAACCCTGAAGCAATCGTGCT AGACATTGACTACAAGTCAGGGACACCAATGCAGAGTGCCGCAAAAGCGCCGTATCTGGCCAAGTTTAAG GTGAAGAGATGCGGAGTGAGTGAACTTGAAAAAGAAGGTCTGCAGTGCCGCTCAGACGCAGAGGATGAAT GCTTCAGCCAAGAGGCTGATGGCAAGAAGATCTGCTGGCAAGCAGCCATCTTCAAAGTGGGGGATGACTG CCGACAGGACATGTTAGCCCTGCAGATCATCGACCTCTTCAAGAACATCTTCCAGCTGGTTGGCCTGGAC CTCTTCGTTTTTCCTTATCGGGTAGTGGCTACTGCCCCTGGGTGCGGAGTGATTGAGTGCATCCCAGACT GTACCTCCCGGGACCAGCTGGGCCGCCAGACTGACTTTGGCATGTATGACTACTTTACACGCCAGTATGG GGATGAGTCTACCTTGGCCTTCCAGCAGGCACGCTACAACTTTATCAGAAGCATGGCCGCCTATAGCCTC CTGCTGTTCCTGCTGCAGATCAAGGACAGGCACAATGGCAACATCATGCTGGACAAGAAGGGGCACATCA TCCACATCGACTTTGGCTTCATGTTTGAAAGCTCACCGGGTGGCAACCTTGGCTGGGAACCAGACATCAA GCTGACAGACGAGATGGTGATGATTATGGGGGGCAAAATGGAAGCCACACCCTTCAAGTGGTTCATGGAG ATGTGTGTCCGAGGTTATCTGGCTGTGCGCCCCTATATGGATGCAGTCGTCTCCCTGGTTACACTCATGT TGGACACAGGCCTGCCCTGTTTTCGTGGCCAGACAATCAAACTTTTGAAACACAGGTTCAGCCCGAACAT GACAGAGCGGGAGGCTGCGAATTTTATCATGAAGGTCATCCAGAACTGTTTCCTCAGCAACAGGAGCCGG ACCTACGACATGATCCAGTACTACCAGAATGATATCCCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212108 representing NM_001001983
Red=Cloning site Green=Tags(s) MCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLRGLPKVYWVEESTARKGRGNLPVA ESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYS NSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPER GIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKK IVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFN MSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAH LPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSEST LNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIR ALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASS VVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPAL KASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIAT KSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYR LEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHA QFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAP YRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESIL HFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLI TALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPF MREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVE IFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSC PPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPL SSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANW RSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHT IDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGY VREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFF NKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFK VKRCGVSELEKEGLQCRSDAEDECFSQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLD LFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSL LLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFME MCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQNCFLSNRSR TYDMIQYYQNDIPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001001983 |
ORF Size | 6132 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001001983.1, NM_001001983.2, NP_001001983.2 |
RefSeq Size | 6449 bp |
RefSeq ORF | 6135 bp |
Locus ID | 224020 |
Cytogenetics | 16 A3 |
MW | 231.4 kDa |
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